Lymphozyten und Zytokine (V 09–V 14)

Wittmann, Miriam; Köck, Reiner; Kienlin, Petra; Kapp, Alexander; Werfel, Thomas; Beschmann, Heike A.; Andrian, Ulrich H. von; Kaufmann, Roland; Holtmeier, Wolfgang; Pfänder, Michael; Hennemann, Andreas; Zollner, Thomas M.; Caspary, W. F.; Göllner, Gerhard P.; Moller, G; Alt, Ruth; Knop, Jürgen; Enk, Alexander; Horst, Alexander; Schmitz, Jürgen; Assenmacher, Mario; Hunzelmann, Nicolas; Kruse, S; Förster, J.; Kuehr, J.; Deichmann, Klaus A.

doi:10.1007/bf03373099

Cited by 1 publication

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“…We and others have previously shown that C5a suppresses IL-12 production in IFN-c-primed and LPS-stimulated monocytes (27)(28)(29) and speculated whether C3a might have a similar effect. Human-purified monocytes were primed with IFN-c (10 ng ⁄ ml) for 1 h and then stimulated with C3a (1 lg ⁄ ml) along with LPS (50 ng ⁄ ml).…”

Section: C3 C3a and Neutralizing Cr1 ⁄ Cr2 Mab Do Not Inhibit Cytokmentioning

confidence: 86%

A protective role of complement component 3 in T cell‐mediated skin inflammation

Purwar

Bäumer

Niebuhr

et al. 2011

Experimental Dermatology

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Keratinocytes synthesize complement component 3 (C3) constitutively, and increased expression of C3 has been described during skin inflammation. In this study, we investigated the role of C3 in T cell-mediated allergic contact dermatitis, which is a clinical manifestation of contact sensitivity (CS). C3-deficient mice (C3KO) showed substantial higher CS responses to haptens, inducing a Th1 cytokine-mediated skin inflammation (2,4-dinitrofluorobenzene and dinitrochlorobenzene), and to haptens known to induce a Th2-polarized inflammatory response (fluoro-isothiocynate and toluene-2,4-diisocyanate) as compared to their wild-type (WT) controls. There was a higher influx of GR-1(+) , CD4(+) , and CD8(+) cells into the skin of hapten-treated C3KO mice compared with WT mice. Activated splenocytes from C3KO mice immunized with DNCB secreted higher amounts of IFN-γ compared with WT controls but not of Th2 (IL-4, IL-5, and IL-10) cytokines or IL-17. A higher secretion of IL-12 from splenocytes of C3KO mice as compared with WT mice was observed after TLR-4 ligand (LPS) or TLR-2 ligand (peptidoglycan) stimulation. Thus, an increased expression of IL-12 and of IFN-γ may be responsible for the increased hapten-induced inflammation in C3 deficiency. Finally, we demonstrated that C3KO mice developed oral tolerance to haptens to a lower degree than WT mice. Our findings provide a new insight into a novel anti-inflammatory role of C3 in skin inflammation.

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Section: C3 C3a and Neutralizing Cr1 ⁄ Cr2 Mab Do Not Inhibit Cytokmentioning

confidence: 86%