“Lyophilisomes”: A New Type of (Bio)capsule

Nanoparticles of nickel oxide and nickel hydroxide using lyophilisomes of fibrinogen as template

“…The second step of annealing at À10 to À20°C could cause macromolecular motions that could result in the more energetically favorable spherical structures. This has been demonstrated in the work of Kuppevelt and co-workers [27]. However since the population distributions of different conformations of the protein at the beginning of the experiment are not exactly controlled, differently extended sheets could result in different sized spherical capsules, thus resulting in the size distributions.…”

“…Stable microcapsules of fibrinogen were prepared using 1 mg/mL of stock solution at pH 7.5 (phosphate buffer 10 mM) using the [27] where proteins are formed into capsules using a specific sequence of freezing at liquid N 2 temperature, followed by rapid heating to À10°C and subsequently a slow annealing to room temperature.…”

“…In this work, we describe the preparation of fibrinogen-nickel chloride microcapsules as a template for preparation of nanoclusters of nickel oxide. The metal-complexed fibrinogen microcapsules were prepared using the method of Kuppevelt and co-workers [27], varying a number of parameters to determine optimal conditions. The preparation method chosen resulted in stable microcapsules of fibrinogen and a particle size of about 28 nm.…”

Elastin as a biomaterial for tissue engineering

“…However, we recently showed the preparation of vesicles (250 nm -10 µm in diameter) from solubilised elastin (Fig. 9A, B) by a novel approach combining freezing and lyophilisation procedures where amphilicity is not necessary [221]. Vesicle formation was critically dependent on elastin concentration, solvent, freezing mode and pressure conditions during lyophilisation.…”

Studies on pH-Controlled Transition of Myoglobin Capsules from Hollow to Multilayered Structures

“…Cavitation methods, complex coacervation and interfacial polymerization are some of the well-established techniques used to prepare capsules of macromolecules (Dӓhne et al 2001;Decher et al 1994;Dobashi et al 1995;Lvov et al 1993). Daamen et al (2007) have shown that biocapsules can be prepared from a range of (large) biomolecules at low temperatures. This method is physical rather than chemical in nature, and amphiphilicity of the biomolecule is not essential.…”