Lyophilization of Brucella melitensis1

Heckly, Robert J.; Faunce, Kenneth; Elberg, Sanford S.

doi:10.1128/aem.8.1.52-54.1960

Cited by 4 publications

(2 citation statements)

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“…54:17-18, 1947). Heckley et al (6) reported that survival of lyophilized cells of Brucella melitensis stored at 0 C was 1,000 times greater than for those stored at 37 C.…”

Section: Resultsmentioning

confidence: 99%

Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells1

Lingg¹,

Mcmahon²,

Herzmann³

1967

Applied Microbiology

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The per cent viability of Bacillus popilliae after lyophilization of liquid nitrogen frozen cells was determined. Lyophilization of 9to 12-hr cells which had been suspended in 5% sodium glutamate plus 0.5% gum tragacanth, frozen in liquid nitrogen vapor, and dried 4 to 5 hr with the ampoules exposed to room temperature resulted in survival of 64.6% of the original cells. After storage of these lyophilized preparations for 6 months at room temperature, 10.5% of the original cells were still viable. Spores of Bacillus popilliae Dutky, the causative agent of type A milky disease of the Japanese beetle, Popillia japonica Newman, have been used as a successful biological control agent (2). However, since sporulation of B. popilliae in vitro has not been able to produce spores in quantity, the possibility of infecting larvae with stabilized vegetative cells in soil is of interest. Milky disease bacteria withstand lyophilization without change in their morphological and cultural characteristics, and their ability to initiate disease in susceptible hosts is not destroyed (5). Lyophilized cultures will remain viable for at least 20 months (5), but information on the rate of survival has not been reported. This study was undertaken to determine the viability of B. popilliae that had been frozen in liquid nitrogen and lyophilized.

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“…54:17-18, 1947). Heckley et al (6) reported that survival of lyophilized cells of Brucella melitensis stored at 0 C was 1,000 times greater than for those stored at 37 C.…”

Section: Resultsmentioning

confidence: 99%

Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells1

Lingg¹,

Mcmahon²,

Herzmann³

1967

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Most stock-culture work has been conducted on a qualitative basis, and, on this basis, stock of many species of microorganisms are known to remain viable through 20 or more years. Among the literature reports giving quantitative data on stability of bacterial preparations during storage are those of Riley and Solowey (1958) for survival of Serratia marcescens at 5 C; Mead et al (1960) for stability of Pasteurella pestis at 5 and -23 C; and Heckley, Anderson, and Rockenmacher (1958) and Heckley, Faunce, and Elberg (1960), who reported increased survival of freeze-dried P. pestis and Brucella melitensis when carbohydrates were added to cultures prior to drying.…”

mentioning

confidence: 99%