Lyophilization of cholesterol-free PEGylated liposomes and its impact on drug loading by passive equilibration

Chaudhury, Anumita; Das, Surajit; Lee, Ronald F. S.; Tan, Kuan-Boone; Ng, Wai Kiong; Tan, Reginald B. H.; Chiu, Gigi Ngar Chee

doi:10.1016/j.ijpharm.2012.04.036

Cited by 49 publications

(28 citation statements)

References 30 publications

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“…Therefore, cryoprotectants were incorporated in the aqueous phase of liposome or dissolved in freshly prepared liposomes. Trehalose as cryoprotectant has proved more effective in inhibiting the growth of liposome particles during freezedrying process 14 . Trehalose dihydrate (20% w/v) was added as cryoprotectant to liposomes (5:1 molar ratio sugar:lipid).…”

Section: Stability Of Immunoliposomesmentioning

confidence: 99%

“…Morphological shape of rhodamine-loaded MPB-PE liposome was depicted in Figure 3(B). We employed the Stewart assay to determine the amount of phospholipids [polyethylene glycol-phosphatidylethanolamine (PEG-PE), phosphatidylglycerol (PG) and MPB-PE] in vesicles, as was previously described 13,14,21 . Therefore, the results obtained show a recovery of 54% w/v of phospholipids [Phosphatidylcholine (PC), PE and PE-MPB].…”

Section: Characterization Of Liposomesmentioning

confidence: 99%

“…CLSM was used to directly visualize IgG coupled to liposomes. IgG-MPB-liposomes loaded with rhodamine B in the aqueous compartment were visualized using the transmitted light 14,22,23 . Confocal analysis was performed to analyze the liposome morphology, to confirm their mean diameter determined by PCS and to verify the linkage between IgG-FITC and the liposomal bilayer.…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

“…Lyophilization is technique used to improve the physical stability of liposomes 14 . Lyophilization constitutes an excellent method to ensure long-term stability of sensitive pharmaceuticals, such as immunoliposomes 15,26 .…”

Section: Stability Of Immunoliposomesmentioning

confidence: 99%

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Surface functionalizing of a lipid nanosystem to promote brain targeting: step-by-step design and physico-chemical characterization

Cózar-Bernal

García-Esteban

Soto

et al. 2015

Pharmaceutical Development and Technology

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The use of lipid nanosystems as drug delivery to the central nervous system may be advantageous over the current strategies. The aim of this study was to develop and characterize functionalized liposomes for treatment of brain diseases. The covalent method of coupling IgG to liposomes via the derivatized lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide](MPB-PE) was investigated. Optimized coupling conditions are shown to result in the efficient conjugation of IgG to liposomes containing low concentrations of MPB-PE (3/1 SH:IgG). The qualitative analysis has shown that after the extrusion process, more homogeneous populations of vesicles have been obtained with a nanometric size suitable to be effective to further anchor the protein. Negative values of zeta potential demonstrate that they are stable systems. Lyophilization was used to maintain the stability of the formulation. These very interesting results encourage further investigations to formulate peptide- and protein-loaded immunoliposomes, making targeting of liposomes as an attractive approach for brain drug delivery.

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Section: Stability Of Immunoliposomesmentioning

confidence: 99%

Section: Characterization Of Liposomesmentioning

confidence: 99%

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

Section: Stability Of Immunoliposomesmentioning

confidence: 99%

See 2 more Smart Citations

Surface functionalizing of a lipid nanosystem to promote brain targeting: step-by-step design and physico-chemical characterization

Cózar-Bernal

García-Esteban

Soto

et al. 2015

Pharmaceutical Development and Technology

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“…Cholesterol (as shown in Fig. 1), which was first named 200 years ago [6], has often been chosen to prepare liposomes together with phospholipids because it can provide a number of formulation benefits, including good retention of encapsulated drug, reduced plasma protein binding and resilience to macrophage attack [4,31].…”

Section: Introductionmentioning

confidence: 99%

Structural properties of paeonol encapsulated liposomes at physiological temperature: Synchrotron small-angle and wide-angle X-ray diffraction studies

Sun

Zhao

et al. 2016

BSI

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Abstract. Structural properties of paeonol-encapsulated liposomes containing cholesterol or stigmasterol at 37°C have been investigated by synchrotron small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) techniques. We compared the structural properties of pure dipalmitoylphosphatidylcholine (DPPC) liposomes, sterol-DPPC liposomes, and those of paeonol-sterol-DPPC liposomes at different molar ratios. Three conclusions can be drawn: First, phase separation occurs in both sterol-DPPC and paeonol-sterol-DPPC liposomes. Second, the incorporation of paeonol molecules into sterol-DPPC liposomes weakens the membrane order. Third, cholesterol has a stronger tendency to interact with DPPC as compared to its counterpart in plant, stigmasterol.

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Introduction

Lakkis¹

2016

Encapsulation and Controlled Release Technologies in Food Systems

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Lyophilization of cholesterol-free PEGylated liposomes and its impact on drug loading by passive equilibration

Cited by 49 publications

References 30 publications

Surface functionalizing of a lipid nanosystem to promote brain targeting: step-by-step design and physico-chemical characterization

Surface functionalizing of a lipid nanosystem to promote brain targeting: step-by-step design and physico-chemical characterization

Structural properties of paeonol encapsulated liposomes at physiological temperature: Synchrotron small-angle and wide-angle X-ray diffraction studies

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