Lyophilization pretreatment facilitates extraction of soluble proteins and active enzymes from the oil-accumulating microalga Chlorella vulgaris

Unterlander, Nicole; Champagne, Pascale; Plaxton, William C.

doi:10.1016/j.algal.2017.06.010

Cited by 20 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) However, Unterlander et al [33] showed that lyophilization prior to lysis using a French press or other methods resulted in substantial increases in the soluble protein concentration and level of active enzymes from C. vulgaris. A small hole in the cell wall is sufficient to release the contents of the cells [34].…”

Section: Morphological Changes In the Cellsmentioning

confidence: 99%

Microalgal cell disruption: Effect on the bioactivity and rheology of wheat bread

et al. 2020

View full text Add to dashboard Cite

The aim of this study is to investigate the potential of the addition of a microalgal biomass to improve nutritional quality of bread. Microalgae contain a substantial amount of nutrients that are naturally encapsulated within cells, namely proteins, polysaccharides, polyunsaturated fatty acids and pigments (chlorophylls and carotenoids), which are capable of resisting harsh conditions during food processing. However, the cell wall integrity may significantly limit nutrient availability, and microalgal cell disruption is potentially required as a pretreatment.A suspension of a fresh Chlorella vulgaris biomass (0.88 g/L) was disrupted in a high-pressure homogenizer at 340 MPa using 1 and 4 passages. The impact of the cell disruption method was evaluated in terms of the reduction in the number of intact cells and average colony diameter of the remaining cells using flow cytometry and microscopy.Since cell disruption promotes the release of intracellular products, it can impart structural modifications to doughs and breads. Therefore, doughs and breads were prepared with the fresh C. vulgaris biomass (1.0 g of Cv/ 100 g of flour+Cv), the disrupted biomass, or a commercial powder. Doughs were characterized in terms of texture and oscillatory rheology. The texture and colour of breads were also evaluated. Cell wall disruption affected the colour and texture of the breads, resulting in breads with a higher firmness. Furthermore, bioactivity was evaluated, and the reducing power of the bread extracts obtained using the ferric ion reducing antioxidant power assay showed that cell disruption positively modulated the antioxidant capacity.

show abstract

Section: Morphological Changes In the Cellsmentioning

confidence: 99%

Microalgal cell disruption: Effect on the bioactivity and rheology of wheat bread

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It can be seen that the biomolecules were also present in the aqueous phase of rehydrated lyophilized cells. This is a trend that has already been observed because the formation of pores on cell walls during lyophilization facilitates the release of biomolecules when cells are resuspended in water …”

Section: Resultsmentioning

confidence: 60%

Membrane‐assisted biorefinery of microalgae to obtain enriched fractions of bioderived molecules

Djamai

Mazzei

Bazzarelli

et al. 2019

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Microalgae represent an important source of valuable bioproducts (such as food, nutraceuticals, antioxidants, and pigments) and biofuel. They grow spontaneously in surface water and represent a contaminant that has to be separated. There is thus an urgent need to develop technologies to harvest and refine them. Depending on the microalgae species, different biocompounds can be found and appropriate separation and purification methods must be refined and selected. In this work, microalgae harvested in the Hammam Boughrara dam, situated in Tlemcen (north-west of Algeria), were used as source to recover and fractionate bio-derived molecules using membrane-assisted processes. This study identified a suitable strategy to fractionate biomolecules using ultrafiltration (UF) membranes. To maintain a stable permeate flux it was necessary to decrease the complexity of the solution prior to UF. This was achieved by separating triacylglycerols (TAGs) and pigments, after breaking cells, by solid-liquid-liquid extraction with a green solvent. The UF of the remaining aqueous phase permitted a recovery factor of 70% of the proteins and more than 85% of the carbohydrates in the retentate. The TAGs that were not recovered in the green solvent, and remained in the aqueous phase, were separated in the permeate with a purity of about 70% and a recovery factor of about 60%.

show abstract

“…Lyophilization dehydrates frozen samples by exposing them to low pressure and a temperature of −50 °C, causing ice crystals to sublimate. This method is commonly used for heat-sensitive materials [ 40 ], such as PC, a pigment protein with a stability temperature of less than 45 °C [ 31 , 41 ]. A significant amount of PC’s blue color remained after drying using this method, as shown in Figure 1 B.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Melanin Production by Streptomyces antibioticus NRRL B-1701 Using Arthrospira (Spirulina) platensis Residues Hydrolysates as Low-Cost L-tyrosine Supplement

Kraseasintra

Sensupa

Mahanil

et al. 2023

BioTech

View full text Add to dashboard Cite

Melanin is a functional pigment that is used in various products. It can be produced by Streptomyces antibioticus NRRL B-1701 when supplemented with L-tyrosine. Arthrospira (Spirulina) platensis is a cyanobacterium with high protein content, including the protein phycocyanin (PC). During PC’s extraction, biomass residues are generated, and these residues still contain various amino acids, especially L-tyrosine, which can be used as a low-cost supplement for melanin production. Thus, this study employed a hydrolysate of A. platensis biomass residue for L-tyrosine substitution. The effects of two drying methods, namely, lyophilization and dying via a hot air oven, on the proximate composition and content of L-tyrosine in the biomass residue were evaluated. The highest L-tyrosine (0.268 g L-tyrosine/100 g dried biomass) concentration was obtained from a hot-air-oven-dried biomass residue hydrolysate (HAO-DBRH). The HAO-DBRH was then used as a low-cost L-tyrosine supplement for maximizing melanin production, which was optimized by the response surface methodology (RSM) through central composite design (CCD). Using the RSM–CCD, the maximum level of melanin production achieved was 0.24 g/L, which is approximately four times higher than it was before optimization. This result suggests that A. platensis residue hydrolysate could be an economically feasible and low-cost alternative source of L-tyrosine for the production of melanin.

show abstract

Lyophilization pretreatment facilitates extraction of soluble proteins and active enzymes from the oil-accumulating microalga Chlorella vulgaris

Cited by 20 publications

References 33 publications

Microalgal cell disruption: Effect on the bioactivity and rheology of wheat bread

Microalgal cell disruption: Effect on the bioactivity and rheology of wheat bread

Membrane‐assisted biorefinery of microalgae to obtain enriched fractions of bioderived molecules

Optimization of Melanin Production by Streptomyces antibioticus NRRL B-1701 Using Arthrospira (Spirulina) platensis Residues Hydrolysates as Low-Cost L-tyrosine Supplement

Contact Info

Product

Resources

About