Lyophilization - Process and Optimization for Pharmaceuticals

Bisht, Deepak; Iqbal, Zeenat

doi:10.22270/ijdra.v3i1.156

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…BacChym is the heat sensitive protein and loses its milk-clotting activity when heated above 50 °C [ 30 ]. Therefore, an effective way to obtain a dry form of the enzyme is sublimation [ 31 ]. To retain their properties, proteins need the correct tertiary structure, which can be disrupted during the freezing process.…”

Section: Discussionmentioning

confidence: 99%

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Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare’s Milk

Akishev

Aktayeva

Kiribayeva

et al. 2022

Biology

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In the cheese-making industry, commonly chymosin is used as the main milk-clotting enzyme. Bactrian camel (Camelus bactrianus) chymosin (BacChym) has a milk-clotting activity higher than that of calf chymosin for cow’s, goat’s, ewes’, mare’s and camel’s milk. A procedure for obtaining milk-clotting reagent based on recombinant camel chymosin is proposed here. Submerged fermentation by a recombinant yeast (Pichia pastoris GS115/pGAPZαA/ProchymCB) was implemented in a 50 L bioreactor, and the recombinant camel chymosin was prepared successfully. The activity of BacChym in yeast culture was 174.5 U/mL. The chymosin was concentrated 5.6-fold by cross-flow ultrafiltration and was purified by ion exchange chromatography. The activity of the purified BacChym was 4700 U/mL. By sublimation-drying with casein peptone, the BacChym powder was obtained with an activity of 36,000 U/g. By means of this chymosin, cheese was prepared from cow’s, goat’s, ewes’, camel’s and mare’s milk with a yield of 18%, 17.3%, 15.9%, 10.4% and 3%, respectively. Thus, the proposed procedure for obtaining a milk-clotting reagent based on BacChym via submerged fermentation by a recombinant yeast has some prospects for biotechnological applications. BacChym could be a prospective milk-clotting enzyme for different types of milk and their mixtures.

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Section: Discussionmentioning

confidence: 99%

“…Nonetheless, for commercial cheese production, the milk-clotting enzyme must be purified and concentrated to reduce the volume of the enzyme solution that has to be added into milk [ 32 ]. Moreover, lyophilization enables biotechnologists to increase the shelf-life of the enzyme and simplify the calculation of its dose [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare’s Milk

Akishev

Aktayeva

Kiribayeva

et al. 2022

Biology

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“…Proses pengeringan vakum dapat menjaga kandungan gizi, bentuk, warna dan kandungan biologis serta struktur produk. Proses pengeringan terdiri dari 3 proses utama yaitu proses pembekuan, proses pengeringan primer dan proses pengeringan sekunder 28,29 .…”

Section: Metode Penelitianunclassified

Liofilisasi Hemolisat Packed Red Cell Limbah Darah Palang Merah Indonesia dengan Penambahan Lioprotektan sebagai Alternatif Standar Hemoglobin

Supriyanta,

Rahmawati,

Martsiningsih

et al. 2024

JKT

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Lyophilization of PRC waste added with 20% sucrose as lyoprotectant. This study aimed to create an alternative Hb standard, replacing the manufacturer's Hb standard. This study used an experimental with pre and posttest without control group design. PMI blood waste PRC was processed into hemolysate, added with lyoprotectant, lyophilized, checked Hb levels using the Cyanmethemoglobin method as a grouppre test, stored at 4OC for six months, checked Hb levels with the Cyanmethemoglobin method as a grouppost test. Homogeneity Test and Stability Test were carried out according to ISO 13528:2015. The average Hb level of PRC lyophilisate before storage (Xr)=15.06 g/dl, obtained Ss≤0.3σpt, so that it was declared homogeneous according to the homogeneous acceptability value according to ISO 13528: 2015. After being stored for six months at 4OC, the average level of Hb lyophilisate PRC (Yr) =14,68 g/dl, obtained |Xr-Yr| ≤ 0.3σpt so that it was declared stable according to the stable acceptability value according to ISO 13528: 2015. Hb levels of PRC lyophilisate were stated to be homogeneous and stable for six months. Hb standard from PRC lyophilisate can be used as an alternative to commercial Hb standard.

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“…Furthermore, as evident from the results of antioxidant activity analysis and total polyphenol content, lyophilization significantly influences the stability of metabolites present in the investigated plant material. Freeze-drying is the method for plant material drying allowing dried material of a much higher quality than in the case of other drying methods [28,29]. Convection air drying is the most commonly used method but it usually takes a long time.…”

Section: Hplc-dad Analysesmentioning

confidence: 99%

Comparative Study of the Phytochemical Profile and Biological Activity of Ajuga reptans L. Leaf and Root Extracts

Dziki,

Malinowska,

Szopa

et al. 2024

Applied Sciences

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In this work, the phytochemical composition and the biological activity of the ethanolic extracts obtained from Ajuga reptans L. (Lamiaceae) leaves and roots (growing in Lesser Poland Voivodeship, Poland) were compared. The phytochemical composition of the extracts were determined by the high-performance liquid chromatography with diode-array detection (HPLC–DAD) method. The dominant compounds in both extracts were verbacoside, isoverbacoside, 3,4-dihydroxyphenylacetic acid and rosmarinic acid. The antioxidant capacity of the extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2’-azobis(3-ethylbenzothiazoline-6-sulfonate (ABTS) tests. The Folin–Ciocalteu assay was used to determine the total polyphenolic content. Additionally, enzyme (tyrosinase, collagenase) inhibition tests and metal chelating ability were studied to assess the anti-aging properties of the extracts. Moreover, the A. reptans extracts’ capacity to absorb the whole range of ultraviolet radiation and high-energy visible (HEV) light was evaluated. The skin irritation test (SIT) EpiDerm was applied to evaluate the safety of the bugle extracts. The noteworthy point is that there is a lack of literature on the assessment of A. reptans root extract activity. Our study is the only one that compares the quantitative composition and biological activity of extracts from the root and leaves of A. reptans. The obtained results indicated that both of the extracts exhibit high antioxidant, chelating and photoprotective activity, but the extract from A. reptans roots showed a higher enzyme inhibition effect for mature skin. The A. reptans root extract, similarly to the leaf extract, could be applied as potentially multi-functional, safe and sensitive cosmetic raw materials, especially in anti-aging and anti-pollution cosmetics.

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Lyophilization - Process and Optimization for Pharmaceuticals

Cited by 11 publications

References 21 publications

Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare’s Milk

Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare’s Milk

Liofilisasi Hemolisat Packed Red Cell Limbah Darah Palang Merah Indonesia dengan Penambahan Lioprotektan sebagai Alternatif Standar Hemoglobin

Comparative Study of the Phytochemical Profile and Biological Activity of Ajuga reptans L. Leaf and Root Extracts

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