Lypopolysaccharide Downregulates the Expression of Selected Phospholipase C Genes in Cultured Endothelial Cells

Vasco, Vincenza Rita Lo; Leopizzi, Martina; Chiappetta, Caterina; Puggioni, Chiara; Rocca, Carlo Della; Polonia, Patrizia; Businaro, Rita

doi:10.1007/s10753-013-9613-3

Cited by 21 publications

(35 citation statements)

References 36 publications

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“…PLC enzymes are described as highly tissue-specific in normal tissues (18,19,21). However, the PLC expression panels in tissues vary under abnormal conditions compared to normal counterparts (20,21,23,24,(26)(27)(28). Moreover, the subcellular distribution of PLC enzymes seems to play Avicenna J Med Biochem.…”

Section: Discussionmentioning

confidence: 99%

“…Knowledge of the expression panels of PI-PLC enzymes is a necessary and preliminary tool to address studies about their role in quiescent cells. Moreover, the expression panels of PI-PLC enzymes have been demonstrated to differ in quiescent cells with respect to the pathological or activated counterpart (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Vasco¹,

Leopizzi²,

d’Abusco³

et al. 2016

Avicenna J Med Biochem

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Background: A large number of phospholipase C (PLC) enzymes, both mRNA transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. MG-63 and SaOS-2 human osteosarcoma cell lines are actually considered osteoblast-like cells, and are therefore widely used as experimental models for osteoblasts. Objectives: Our aim was to verify whether MG-63 or SaOS-2 cells might also represent appropriate experimental osteoblast models for signal transduction studies, with special regard to the phosphoinositide (PI) pathway. We analyzed the expression and the subcellular distribution of enzymes related to calcium signal transduction (the PI-specific PLC family), which are known to possess high cell/tissue specificity. Materials and Methods:The expression of PLC genes was analyzed by performing RT-PCR experiments. The presence of PLC enzymes and their subcellular distribution within the cells was analyzed with immunofluorescence experiments. Results: Osteoblasts, MG-63 cells, and SaOS-2 cells have expression panels similar to those of PLC enzymes. However, slight differences were found in the expression of enzymes belonging to the PLCη subfamily. Conclusions: MG-63 and SaOS-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Vasco¹,

Leopizzi²,

d’Abusco³

et al. 2016

Avicenna J Med Biochem

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“…PLCH1 is a member of the phospholipase enzyme family that generates the secondary messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) by cleaving phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Phospholipases were shown to be involved in in ammation mechanisms [43], especially the expression of PLCH1 which was shown to be downregulated by lipopolysaccharides (LPS) [44] which are found in the outer membrane of Gram-negative bacteria [45]. The region associated with relative abundance of Treponema spp.…”

Section: Discussionmentioning

confidence: 99%

The Bovine Foot Skin Microbiota is Associated with Host Genotype and the Development of Infectious Digital Dermatitis Lesions

Bay¹,

Gillespie

Ganda

et al. 2021

Preprint

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Bovine Digital Dermatitis (BDD) is a prevalent infectious disease, causing painful foot skin lesions and lameness in cattle. The polymicrobial nature of this disease has led to the hypothesis that the foot skin microbiota may be associated with occurrence and progression of lesions. We describe herein the bovine foot skin microbiota using 16S rRNA gene amplicon and shotgun metagenomic sequencing on samples from 259 dairy cows from three UK dairy farms. We show differences in the foot skin microbiome profiles of clinically healthy animals that were associated with subsequent development of BDD. We also present the first co-occurrence analysis of the bovine foot skin microbiome showing ecological relationships among bacterial species. Taxonomical and functional differences together with alterations in ecological interactions between bacteria in the normal foot skin microbiome may predispose an animal to develop BDD lesions. Using genome-wide association and regional heritability mapping approaches, we provide first evidence for interactions between host genotype and the foot skin microbiota profiles. We show the existence of genetic variation in the relative abundance of Treponema spp. and Peptoclostridium spp. and identify regions in the bovine genome that explain a significant proportion of this variation.

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“…The panel of PLC expression in each cell varies under abnormal conditions, compared to the normal counterparts (43, 59,60). The panel of PLC expression was also modified by specific stimuli (61)(62)(63), including inflammation (64)(65)(66), or by some specific inhibitors (67,68). The subcellular distribution of the PLC enzymes influences their activity, suggesting specific roles for each isoform besides the cleavage of PIP2 (69)(70)(71)(72)(73).…”

Section: ) (37)mentioning

confidence: 99%

“…The subcellular distribution of the PLC enzymes influences their activity, suggesting specific roles for each isoform besides the cleavage of PIP2 (69)(70)(71)(72)(73). The PLC enzymes are involved in some human diseases, including the nervous system tumors (64,65) and abnormalities (64,(74)(75)(76)(77)(78)(79).…”

Section: ) (37)mentioning

confidence: 99%

Phosphoinositide Signal Transduction Pathway and Osteosarcoma Metastases

Vasco

Rita²

2021

Jentashapir J Cell Mol Biol

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: Metastasis spreading confers a worse prognosis to the clinical outcome among patients suffering from osteosarcoma, the most common primary bone tumor in childhood and adolescence. The detection of molecules involved in metastasis spreading might contribute to understanding tumor dissemination mechanisms, thereby opening the way to novel therapeutic strategies. The Ezrin-radixin-moesin (ERM) family proteins are activated after interacting with molecules belonging to the phosphoinositide signal transduction pathway. The phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule in the PI pathway, stabilizes the ERM proteins or a more efficient receptor binding. The PIP2 levels in the pathway are a critical element for regulating several cell events. The PIP2 levels are regulated using enzymes, including the PI-specific Phospholipase C family. A decrease in the PIP2 levels induces the dissociation of the ERM protein from the membrane. In this regard, the PI-PLC enzymes regulate this event. In this paper, the role of the PI signal transduction molecules in osteosarcoma metastases is discussed.

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Lypopolysaccharide Downregulates the Expression of Selected Phospholipase C Genes in Cultured Endothelial Cells

Cited by 21 publications

References 36 publications

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

The Bovine Foot Skin Microbiota is Associated with Host Genotype and the Development of Infectious Digital Dermatitis Lesions

Phosphoinositide Signal Transduction Pathway and Osteosarcoma Metastases

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