Lys296 and Arg299 residues in the C-terminus of MD-ACO1 are essential for a 1-aminocyclopropane-1-carboxylate oxidase enzyme activity

Yoo, Ahrim; Seo, Young Sam; Jung, Jin; Sung, Soon Kee; Kim, Woo Taek; Lee, Weontae; Yang, Dae Ryook

doi:10.1016/j.jsb.2006.08.012

Cited by 27 publications

(34 citation statements)

References 47 publications

(80 reference statements)

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“…1b). Because PnACO1 contains motifs that are evolutionarily conserved and characteristic of ACC oxidases and these motifs are located within the Lys and Arg residues essential for protein activity (Yoo et al 2006) (Fig. 1a), it can be suspected that this gene encodes for a functional enzymatic protein.…”

Section: Discussionmentioning

confidence: 99%

“…1a). Based on a comparison between the deduced PnACO1 amino acid sequence and ACC oxidase sequences from other plant species, it was found that the PnACO1 protein, too, contains characteristic motifs: a cofactor binding one (Hsp177-X-Asp179-X(54)-Hsp234) and a co-substrate binding one (Arg244-X-Ser246) (Yoo et al 2006). However, at the C-terminus of the PnACO1 protein, an amino acid sequence was identified that was evolutionarily conserved and characteristic of ACC oxidases, containing the Lys and Arg residues essential for enzymatic activity (positions 294-301).…”

Section: Isolation Of Cdna For the Acc Oxidasementioning

confidence: 99%

“…Small letters are used for regions not subject to translation. Light gray denotes the motif forming the Fe(II) binding pocket comprising three characteristic amino acid residues (in boldface type) (Yoo et al 2006). The ascorbic acid-binding motif comprising two characteristic amino acid residues (underlined and in boldface type) is marked dark gray.…”

Section: Isolation Of Cdna For the Acc Oxidasementioning

confidence: 99%

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The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

et al. 2012

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In this study, the first ACC oxidase (PnACO1) cDNA from model short-day plant Pharbitis nil was isolated. The expression pattern of PnACO1 was studied under different conditions (photoperiod and auxin), an adequate balance of which determines P. nil flowering. It was shown that the gene was transcribed in all the examined organs of the 5-day-old seedling and was strongly activated by auxin. Our results also revealed that PnACO1 transcript accumulation in the cotyledons showed diurnal oscillations under both LD and SD conditions. On the basis of presented and previously obtained data, we suggest that flowering inhibition evoked by IAA in P. nil results from its stimulatory effect on both ACC synthase and oxidase gene expression and, consequently, enhances ethylene production.

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Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Cdna For the Acc Oxidasementioning

confidence: 99%

Section: Isolation Of Cdna For the Acc Oxidasementioning

confidence: 99%

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The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

et al. 2012

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“…S2d). The predicted LlACO sequence and ACC oxidases from other plant species were compared and it was found that the LlACO protein also included conserved motifs: a cofactor and a cosubstrate binding [Hsp177-X-Asp179-X(54)-Hsp234 and Arg244-X-Ser246, respectively] [20] (Fig. S2d).…”

Section: Isolation Of Llacs and Llaco Cdnasmentioning

confidence: 99%

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

et al. 2017

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The abscission of certain organs from the plant is part of the fulfilment of its developmental programs. The separation process occurs in a specialized abscission zone usually formed at the base of detached organ. The changing level of phytohormones, particularly ethylene, is the element responsible for coordinating anatomical and physiological transformation that accompanies organ abscission. The application of ethylene (ET) on Lupinus luteus stimulates flower abortion. However, the treatment with 1-aminocyclopropane-1-carboxylic acid (ACC) -direct ET precursor -does not cause such a strong physiological response. In turn, when applied on the pedicels both ET biosynthesis (2-aminoethoxyvinylglycine; AVG) and action (norbornadiene; NBD) inhibitors reversed the stimulatory effect of ET on generative organ separation. In order to determine ET role in the flower abscission process in L. luteus, we identified the sequences coding for synthase (LlACS) and oxidase (LlACO) of ACC and measured their expression levels. Abscission zone activation is accompanied by a considerable increase both in LlACS and LlACO cDNAs and also ACC content, which is specifically localized in the dividing cells at the base of the flower being detached. Obtained results suggest that ET is a strong stimulator of flower abortion in L. luteus.

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“…The amino acid sequence of allele d revealed the presence of a Fe 2+ -binding motif (His-177-X-Asp-179-X(54)-His-234), putative co-substrate hydrogen-binding residues (Arg-244-X-Ser-246), and both Lys-296 and Arg-299 residues in the C-terminal helix, all of which play a key role in the activity of the enzyme (Yoo et al 2006). …”

Section: Cloning and Sequencing Of Allele D Of The Aco1 Genementioning

confidence: 99%

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Maric¹

2016

Hort. Sci. (Prague)

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Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This paper presents the results of molecular cloning and characterization of a newly identified allele of the ripening-specific ACO1 gene from Malus floribunda 821. A polymorphism of the ACO1 gene was detected using the polymerase chain reaction and BamH1 and RsaI enzymatic digestion. Whilst cloning and sequencing data confirmed the identity of allele d, it also revealed high sequence conservation (97.3 to 98.9%). In addition, a comparison of sequence data revealed some differences within the coding regions of the ACO1 alleles identified so far.

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Lys296 and Arg299 residues in the C-terminus of MD-ACO1 are essential for a 1-aminocyclopropane-1-carboxylate oxidase enzyme activity

Cited by 27 publications

References 47 publications

The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

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