Lysed Enterococcus faecalis FK-23 (LFK) Suppressing Allergic Responses in Mouse Models

Shimada, Takashi; Cheng, Long; Motonaga, Chie; Shi, Haibo; Yamasaki, Akira; Enomoto, Tadao; Shirakawa, Taro

doi:10.2332/allergolint.54.367

Cited by 5 publications

(8 citation statements)

References 40 publications

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“…The strain of mice employed in this work (BALB/c) is known for its tendency to establish Th2 responses [39] and its difficulty to establish persistent Th1 responses, mainly in immunization protocols employing high concentrations of Al(OH) 3 as adjuvant. The latter fact might also account for the lack of secretion of IFN-γ ex vivo.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Studies on the Prevention of the Ovalbumin-Induced Allergic Response by <i>Enterococcus faecalis</i> CECT7121 in Mice

Castro

Azpiroz

Molina

et al. 2011

Int Arch Allergy Immunol

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Background: Allergic diseases are featured by an increased production of IgE due to an imbalance in the immune response towards a Th2 profile. In this work, the ability of Enterococcus faecalis CECT7121 to regulate this Th2-exaggerated response in a murine model of ovalbumin (OVA)-induced allergy was studied. Methods: BALB/c mice intragastrically inoculated with E. faecalis CECT7121 before and during a subcutaneous immunization protocol with OVA were studied in comparison with an immunized control group. The allergen-specific immune response (IgE, IgG, IgG1 and IgG2a) was assessed. The proliferative activity of memory splenocytes and the levels of IL-4, IL-5, IL-13, IL-10, IL-12 and IFN-γ were also determined. Results: Upon treatment with E. faecalis CECT7121 the following effects were observed: (1) a decrease in specific IgE levels, (2) an increase in anti-OVA IgG2a levels, (3) the levels of anti-OVA IgG and IgG1 remained unaltered, (4) a reduction in the proliferation rate of memory cells, (5) a decrease in the levels of the Th2 cytokines IL-4, IL-5 and IL-13, and (6) the secretion of IL-10, IL-12 and IFN-γ remained unchanged. Moreover, the incubation of human basophils with non-viable E. faecalis CECT7121 together with an allergen preparation induced the release of β-hexosaminidase at levels that were lower than control reactions and similar i.g. the spontaneous release. Conclusions: In this model, the i.g. administration of E. faecalis CECT7121 hampers the establishment of the OVA-induced allergic immune response, suggesting that this strain could be useful for the treatment of IgE-mediated allergic diseases.

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Section: Discussionmentioning

confidence: 99%

Preliminary Studies on the Prevention of the Ovalbumin-Induced Allergic Response by <i>Enterococcus faecalis</i> CECT7121 in Mice

Castro

Azpiroz

Molina

et al. 2011

Int Arch Allergy Immunol

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“…However, as OVA is predominantly a food allergen, it might not be especially suitable for modelling other types of allergy, such as allergic airways disease. In that case, models implementing clinically relevant aeroallergens, such as house dust mite proteins ( Steelant et al, 2016 ) and tree pollen ( Schabussova et al, 2012 ; Shimada et al, 2005 ), might have a higher translational relevance.…”

Section: The Use Of Mouse Models In Probiotic Researchmentioning

confidence: 99%

“…The authors linked these differences to lower microbiota diversity in A/J mice, and suggested that the intrinsic microbiota of the host can interfere with the expected probiotic effects. Likewise, local peritoneal accumulation of eosinophils induced by the administration of a Japanese cedar pollen solution was reduced following treatment with the lysed probiotic Enterococcus faecalis FK-23 in BALB/c, C3H/HeN and C3H/HeJ mice, but not in C57BL/6 mice, possibly due to the differences in TLR functionality between the strains ( Shimada et al, 2005 ). However, certain mouse strain-specific effects can be compensated by adjusting the treatment parameters, such as the probiotic dose.…”

Section: Critical Host-related Factorsmentioning

confidence: 99%

Probiotics against airway allergy: host factors to consider

Spacova

Ceuppens

Seys

et al. 2018

Disease Models &Amp; Mechanisms

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The worldwide prevalence of allergic diseases has drastically increased in the past decades. Recent studies underline the importance of microbial exposure for the development of a balanced immune system. Consequently, probiotic bacteria are emerging as a safe and natural strategy for allergy prevention and treatment. However, clinical probiotic intervention studies have so far yielded conflicting results. There is increasing awareness about the importance of host-associated factors that determine whether an individual will respond to a specific probiotic treatment, and it is therefore crucial to promote a knowledge-based instead of an empirical selection of promising probiotic strains and their administration regimen.In this Review, we summarize the insights from animal model studies of allergic disease, which reveal how host-related factors – such as genetic makeup, sex, age and microbiological status – can impact the outcomes of preventive or curative probiotic treatment. We explore why and how these factors can influence the results of probiotic studies and negatively impact the reproducibility in animal experiments. These same factors might profoundly influence the outcomes of human clinical trials, and can potentially explain the conflicting results from probiotic intervention studies. Therefore, we also link these host-related factors to human probiotic study outcomes in the context of airway allergies.

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“…Since a multidrug-resistant E. faecalis strain is refractory to most therapeutic options, it may become a target of bacteriophage therapy (13). Conversely, E. faecalis is an important bacterium in fermentation industries, especially for cheese production (5,8), and the cells are used for dietary supplement production (11). Lysed E. faecalis cells were shown to improve the intestinal flora disturbed by antibiotics (12), to improve clinical symptoms in allergic rhinitis (10), and to suppress active cutaneous anaphylaxis and local accumulation of eosinophils (11).…”

mentioning

confidence: 99%

“…Conversely, E. faecalis is an important bacterium in fermentation industries, especially for cheese production (5,8), and the cells are used for dietary supplement production (11). Lysed E. faecalis cells were shown to improve the intestinal flora disturbed by antibiotics (12), to improve clinical symptoms in allergic rhinitis (10), and to suppress active cutaneous anaphylaxis and local accumulation of eosinophils (11). While bacteriophages should be the natural enemy against pathogenic bacteria, they are the primary cause of fermentation failure in the food, chemical, pharmaceutical, feed, and pesticide industries (3,4).…”

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confidence: 99%

Complete Genome Sequence of Bacteriophage BC-611 Specifically Infecting Enterococcus faecalis Strain NP-10011

Horiuchi

Sakka

Hayashi

et al. 2012

J Virol

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Enterococcus faecalis is an opportunistic pathogen that causes serious infections in humans and animals and is also an important bacterium for dairy and probiotic supplement production. Therefore, bacteriophages infecting E. faecalis may be useful for phage therapy against multidrug-resistant strains or may threaten industrial fermentation. We isolated a virulent Siphoviridae bacteriophage, BC-611, specifically infecting E. faecalis strain NP-10011 but not infecting other E. faecalis strains or other enterococci. Although the genome sequence of BC-611 resembled that of enterococcal bacteriophage SAP6, BC-611 was marked by its narrow host specificity.

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Lysed Enterococcus faecalis FK-23 (LFK) Suppressing Allergic Responses in Mouse Models

Cited by 5 publications

References 40 publications

Preliminary Studies on the Prevention of the Ovalbumin-Induced Allergic Response by <i>Enterococcus faecalis</i> CECT7121 in Mice

Preliminary Studies on the Prevention of the Ovalbumin-Induced Allergic Response by <i>Enterococcus faecalis</i> CECT7121 in Mice

Probiotics against airway allergy: host factors to consider

Complete Genome Sequence of Bacteriophage BC-611 Specifically Infecting Enterococcus faecalis Strain NP-10011

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