Lysine-derivatized polyurethane as a clot lysing surface: conversion of adsorbed plasminogen to plasmin and clot lysis in vitro

McClung, W. Glenn; Clapper, David L.; Hu, S.-P.; Brash, John L.

doi:10.1016/s0142-9612(00)00378-1

Cited by 52 publications

(50 citation statements)

References 21 publications

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“…In this regard, a polyurethane material was covalently coated with polyacrylamide bearing pendant e-lysine and benzophenone moieties by photochemical methods. [35,36] The e-lysine density achieved by this method ranged from 0.2 to 3.2 nmol Á cm À2 depending on the concentration of the coating reagent. Plasminogen adsorption from plasma increased with increasing lysine content and reached a value of 1.2 mg Á cm À2 for the surface with the highest lysine content.…”

Section: Regulation Of Specific Protein/surface Interactions By Surfamentioning

confidence: 99%

Surface Modification to Control Protein/Surface Interactions

Lin

et al. 2011

Macromolecular Bioscience

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Protein/surface interactions are well known to play an important role in various biological phenomena and to determine the ultimate biofunctionality of a given material once it is in contact with a biological environment. Control over the interactions between proteins and material surfaces are not only of great theoretical interest but also of crucial importance for many biomedical applications. In this Feature Article, we summarize various successful approaches used in our laboratory and other groups for controlling protein adsorption through chemical modification of the surface and/or the introduction of specific topographic features. Some perspectives on future research in these areas are also presented.

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Section: Regulation Of Specific Protein/surface Interactions By Surfamentioning

confidence: 99%

Surface Modification to Control Protein/Surface Interactions

Lin

et al. 2011

Macromolecular Bioscience

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“…Moreover, the incorporation of free ε -amino groups on the surface, by using PEG -lysine conjugates, rendered the surface capable of dissolving fi brin clots because of adsorption of the fi brinolytic protein, plasminogen, from blood plasma [70] . Similar studies in the past had shown that polyurethane surfaces coated with a lysine -derivatized acrylamide polymer dissolved fi brin clots by a ready conversion of the adsorbed plasminogen to plasmin in the presence of tissue -plasminogen activator ( TPA ) [71] . The design of these lysine -based anticlotting coatings is based on the fact that surfaces incorporating a high density of lysine residues, in which the ε -amino groups are free, are capable of selective adsorption of plasminogen from blood plasma (up to a level of 1.2 μ g cm − 2 , corresponding to a compact monolayer of plasminogen), and virtually no other proteins [72] .…”

Section: Clot -Lyzing Thin Filmsmentioning

confidence: 56%

Polymer Thin Films for Biomedical Applications

Vendra¹,

Wu²,

Krishnan³

2010

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Biocompatible Coatings Protein‐Repellant Coatings PEG ylated Thin Films Non‐ PEG ylated Hydrophilic Thin Films Thin Films of Hyperbranched Polymers Multilayer Thin Films Antithrombogenic Coatings Surface Chemistry and Blood Compatibility Membrane‐Mimetic Thin Films Heparin‐Mimetic Thin Films Clot‐Lyzing Thin Films Polyelectrolyte Multilayer Thin Films Polyurethane Coatings Vapor‐Deposited Thin Films Antimicrobial Coatings Cationic Polymers Nanocomposite Polymer Thin Films Incorporating Inorganic Biocides Antibiotic‐Conjugated Polymer Thin Films Biomimetic Antibacterial Coatings Thin Films Resistant to the Adhesion of Viable Bacteria Coatings for Tissue Engineering Substrates PEG ylated Thin Films Zwitterionic Thin Films Thin Films of Hyperbranched Polymers Polyurethane Coatings Polysaccharide‐Based Thin Films Polyelectrolyte Multilayer Thin Films Temperature‐Responsive Polymer Coatings Electroactive Thin Films Other Functional Polymer Coatings Multilayer Thin Films for Cell Encapsulation Patterned Thin Films Polymer Thin Films for Drug Delivery Polymer Thin Films for Gene Delivery Conclusions

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“…Other studies on these -lysine surfaces focused on t-PA activation and clot lysing properties [15]. Data based on a chromogenic substrate assay for plasmin showed that plasminogen adsorbed to the -lysine surfaces was readily converted to plasmin in the presence of t-PA.…”

Section: Fibrinolytic Surface With Lysine-rich Coatingsmentioning

confidence: 99%

“…Such a surface would mimic the naturally occurring fibrinolytic process that occurs on the surface of fibrin [8]. This concept has been pursued in our laboratories over the past several years [9][10][11][12][13][14][15][16][17][18][19][20][21]. The present contribution is intended, in part, as an elaboration of this concept and an outline of developments to date.…”

Section: Introductionmentioning

confidence: 99%