2016
DOI: 10.2147/ijn.s92218
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Lysine-functionalized nanodiamonds as gene carriers: development of stable colloidal dispersion for in vitro cellular uptake studies and siRNA delivery application

Abstract: Purpose Nanodiamonds (NDs) are emerging as an attractive tool for gene therapeutics. To reach their full potential for biological application, NDs should maintain their colloidal stability in biological milieu. This study describes the behavior of lysine-functionalized ND (lys-ND) in various dispersion media, with an aim to limit aggregation and improve the colloidal stability of ND-gene complexes called diamoplexes. Furthermore, cellular and macromolecular interactions of lys-NDs are also analyze… Show more

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Cited by 18 publications
(6 citation statements)
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“…However, for most biomedical applications including drug delivery, the precise control of their surfaces via functionalization is necessary [ 83 , 84 , 85 , 86 , 87 ]. It has been pointed out that NDs are not used neat but are conjugated with biocompatible polymers and drugs, similarly to previously reported cases with CNTs and GOs focusing on cancer treatment [ 84 , 88 , 89 , 90 , 91 , 92 ].…”
Section: Types Of Nanocarrierssupporting
confidence: 63%
“…However, for most biomedical applications including drug delivery, the precise control of their surfaces via functionalization is necessary [ 83 , 84 , 85 , 86 , 87 ]. It has been pointed out that NDs are not used neat but are conjugated with biocompatible polymers and drugs, similarly to previously reported cases with CNTs and GOs focusing on cancer treatment [ 84 , 88 , 89 , 90 , 91 , 92 ].…”
Section: Types Of Nanocarrierssupporting
confidence: 63%
“…Perhaps, the most studied application of ND for gene delivery is based on the non-covalent integration of polycationic molecules onto the ND surface followed by association with negatively charged nucleic acids [69,87,152,[178][179][180][181][182][183] (inset, figure 6). For example, pEGFPLuc plasmids encoding Luciferase and green fluorescent protein (GFP) have been successfully delivered in cytoplasm by means of aa ND-PEI vector [178].…”
Section: Application Of Nd In Gene Deliverymentioning
confidence: 99%
“…DLS measurements of proanthocyanidins were performed at 0.1, 1, 5, 10, and 50 μM concentrations in DMEM + 10% FBS (constant 0.5% final DMSO across all samples) using a Brookhaven 90 Plus particle size analyzer. Measurements were conducted after incubating samples for 30 minutes at room temperature (covered to protect from dust particles) using a viscosity of 0.94 cP ( Fröhlich et al, 2013 ), refractive index of 1.345 ( Alwani et al ), wavelength of 658 nm and scattering angle of 90°.…”
Section: Methodsmentioning
confidence: 99%