The aim of current study was to evaluate the effect of different levels of lysine and threonine on performance, carcass characteristics and intestinal microflora of broiler. Nine hundred and sixty Ross 308 one-day-old female broiler chicks were selected, and they were randomly divided in a factorial design with 12 treatments, four replicates and 20 chicks in each replicate. The tested diets included four levels of lysine (100, 110, 120 and 130%, suggested by NRC) and three levels of threonine (100, 110 and 120%, suggested by NRC). Two phase (starter 0-21d; finisher 22-42 d) feeding schedule was followed. Results showed that of 21 days of age, 120% of lysine and threonine the live and carcass weights and breast, thigh and liver yields in chicks increased (p < .05). At the age of 42 days those treatments which received 120% of threonine gained the highest rate of live and carcass weights and yield of carcass characteristics. The intestinal microflora, in 42 days of age, with the increase in concentration of lysine significantly decreased in the intestinal microflora. The level of threonine in diet, and the interaction between threonine and lysine at 42 days of age effect on intestinal microflora. The best feed conversion ratio was related to 120% lysine. According to the results of this study, the carcass characteristics and growth performance can be improved with increasing in lysine and threonine levels to 120% above from NRC recommendations for commercial broilers.
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