Lysine Residues in the MK-Rich Region Are Not Required for Binding of the PbsP Protein From Group B Streptococci to Plasminogen

Coppolino, Francesco; Romeo, Letizia; Pietrocola, Giampiero; Lentini, Germana; Gaetano, Giuseppe Valerio De; Teti, Giuseppe; Galbo, Roberta; Beninati, Concetta

doi:10.3389/fcimb.2021.679792

Cited by 7 publications

(5 citation statements)

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“…One of the most studied TCS in GBS is the CovRS regulatory system where the sensor histidine kinase CovS senses external signals and activates the response regulator CovR [ 220 ]. This system directly or indirectly regulates the expression of different genes [ 221 ], including those encoding virulence factors, such as β-hemolysin/cytolysin (β-h/c) [ 222 ], HvgA [ 223 ], PbsP [ 209 , 224 , 225 , 226 ], and other ones. Furthermore, pbsP is subjected to the transcriptional regulation mediated by the recently discovered SaeRS TCS that greatly enhances its expression by rendering GBS more virulent in vivo [ 216 ].…”

Section: Two-component Systemsmentioning

confidence: 99%

“…Furthermore, pbsP is subjected to the transcriptional regulation mediated by the recently discovered SaeRS TCS that greatly enhances its expression by rendering GBS more virulent in vivo [ 216 ]. Studies on SaeRS correlation with pbsP are currently underway among different clonotypes and in other in vivo infection models, given the importance of this adhesin in GBS pathogenesis [ 209 , 224 , 225 , 226 ].…”

Section: Two-component Systemsmentioning

confidence: 99%

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In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections

et al. 2023

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Two-component signaling systems (TCSs) are finely regulated mechanisms by which bacteria adapt to environmental conditions by modifying the expression of target genes. In bacterial pathogenesis, TCSs play important roles in modulating adhesion to mucosal surfaces, resistance to antibiotics, and metabolic adaptation. In the context of urinary tract infections (UTI), one of the most common types infections causing significant health problems worldwide, uropathogens use TCSs for adaptation, survival, and establishment of pathogenicity. For example, uropathogens can exploit TCSs to survive inside bladder epithelial cells, sense osmolar variations in urine, promote their ascension along the urinary tract or even produce lytic enzymes resulting in exfoliation of the urothelium. Despite the usefulness of studying the function of TCSs in in vitro experimental models, it is of primary necessity to study bacterial gene regulation also in the context of host niches, each displaying its own biological, chemical, and physical features. In light of this, the aim of this review is to provide a concise description of several bacterial TCSs, whose activity has been described in mouse models of UTI.

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Section: Two-component Systemsmentioning

confidence: 99%

Section: Two-component Systemsmentioning

confidence: 99%

In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections

et al. 2023

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“…Amino acids are the important molecules in human body, and its main role is as components of proteins ( Das et al., 2020 ; Farag et al., 2020 ; Hegazi et al., 2020 ; Coppolino et al., 2021 ). However, they are also involved in many important biological functions, such as i) provide energy and ii) serve as neurotransmitters.…”

Section: Discussionmentioning

confidence: 99%

Associations Between Disordered Microbial Metabolites and Changes of Neurotransmitters in Depressed Mice

Xie

Wang

Zhong

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundsMany pieces of evidence demonstrated that there were close relationships between gut microbiota and depression. However, the specific molecular mechanisms were still unknown. Here, using targeted metabolomics, this study was conducted to explore the relationships between microbial metabolites in feces and neurotransmitters in prefrontal cortex of depressed mice.MethodsChronic unpredictable mild stress (CUMS) model of depression was built in this study. Targeted liquid chromatography–mass spectrometry analysis was used to detect the microbial metabolites in feces and neurotransmitters in prefrontal cortex of mice. Both univariate and multivariate statistical analyses were applied to identify the differential microbial metabolites and neurotransmitters and explore relationships between them.ResultsNinety-eight differential microbial metabolites (mainly belonged to amino acids, fatty acids, and bile acids) and 11 differential neurotransmitters (belonged to tryptophan pathway, GABAergic pathway, and catecholaminergic pathway) were identified. Five affected amino acid–related metabolic pathways were found in depressed mice. The 19 differential microbial metabolites and 10 differential neurotransmitters were found to be significantly correlated with depressive-like behaviors. The two differential neurotransmitters (tyrosine and glutamate) and differential microbial metabolites belonged to amino acids had greater contributions to the overall correlations between microbial metabolites and neurotransmitters. In addition, the significantly decreased L-tyrosine as microbial metabolites and tyrosine as neurotransmitter had the significantly positive correlation (r = 0.681, p = 0.0009).ConclusionsThese results indicated that CUMS-induced disturbances of microbial metabolites (especially amino acids) might affect the levels of neurotransmitters in prefrontal cortex and then caused the onset of depression. Our findings could broaden the understanding of how gut microbiota was involved in the onset of depression.

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“…Previous studies have demonstrated both direct and indirect interactions of GBS with integrins. For example, these bacteria can invade respiratory cells by using the Plasminogen binding surface Protein (PbsP) cell wall adhesin to bind vitronectin, which acts as a bridge to activate the α v integrins (Buscetta et al, 2016 ; De Gaetano et al, 2018 ; Lentini et al, 2018 ; Coppolino et al, 2021 ). Direct GBS-integrin interactions have also been documented (Cuzzola et al, 2000 ; Bolduc and Madoff, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Engagement of α3β1 and α2β1 integrins by hypervirulent Streptococcus agalactiae in invasion of polarized enterocytes

De Gaetano,

Lentini,

Coppolino

et al. 2024

Front. Microbiol.

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The gut represents an important site of colonization of the commensal bacterium Streptococcus agalactiae (group B Streptococcus or GBS), which can also behave as a deadly pathogen in neonates and adults. Invasion of the intestinal epithelial barrier is likely a crucial step in the pathogenesis of neonatal infections caused by GBS belonging to clonal complex 17 (CC17). We have previously shown that the prototypical CC17 BM110 strain invades polarized enterocyte-like cells through their lateral surfaces using an endocytic pathway. By analyzing the cellular distribution of putative GBS receptors in human enterocyte-like Caco-2 cells, we find here that the alpha 3 (α3) and alpha 2 (α2) integrin subunits are selectively expressed on lateral enterocyte surfaces at equatorial and parabasal levels along the vertical axis of polarized cells, in an area corresponding to GBS entry sites. The α3β1 and α2β1 integrins were not readily accessible in fully differentiated Caco-2 monolayers but could be exposed to specific antibodies after weakening of intercellular junctions in calcium-free media. Under these conditions, anti-α3β1 and anti-α2β1 antibodies significantly reduced GBS adhesion to and invasion of enterocytes. After endocytosis, α3β1 and α2β1 integrins localized to areas of actin remodeling around GBS containing vacuoles. Taken together, these data indicate that GBS can invade enterocytes by binding to α3β1 and α2β1 integrins on the lateral membrane of polarized enterocytes, resulting in cytoskeletal remodeling and bacterial internalization. Blocking integrins might represent a viable strategy to prevent GBS invasion of gut epithelial tissues.

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Lysine Residues in the MK-Rich Region Are Not Required for Binding of the PbsP Protein From Group B Streptococci to Plasminogen

Cited by 7 publications

References 62 publications

In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections

In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections

Associations Between Disordered Microbial Metabolites and Changes of Neurotransmitters in Depressed Mice

Engagement of α3β1 and α2β1 integrins by hypervirulent Streptococcus agalactiae in invasion of polarized enterocytes

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