Lysine supplementation of commercial fishmeal-free diet in hybrid striped bassMorone chrysops x M. saxatilisaffects expression of growth-related genes

Abstract

Our recent results in hybrid striped bass (HSB) concluded that ideal protein theory accurately predicts first-limiting amino acids in commercial diet formulations if accurate amino acid availability data are used and that appropriate levels of supplemental lysine are needed to improve fish performance from fishmeal-free diets. Our goal in this study was to elucidate how dietary lysine supplementation of a commercial fishmealfree diet influences the expression of two genes, myostatin and myogenin, controlling myogenesis in differentially growing groups of HSB. Real-time RT-PCR results in HSB suggest that the levels of lysine added to the diet (17.8, 35.1, 51.0 g kg À1 of diet) has an impact on myogenin relative to the basal unsupplemented diet, but no effect on myostatin. Moreover, our data further suggest that the amount of dietary lysine supplementation influenced the ratio of myostatin/myogenin expression in HSB and that this pattern mimicked that of most of the growth, composition of growth and nutrient retention data from our previous study and may therefore be a useful marker for selecting fish for improved growth performance.

Leucine Improved Growth Performance, Muscle Growth, and Muscle Protein Deposition Through AKT/TOR and AKT/FOXO3a Signaling Pathways in Hybrid Catfish Pelteobagrus vachelli × Leiocassis longirostris

“…Michelato et al reported that dietary histidine upregulated MyoD and MyoG mRNA expression and affected muscle hyperplasia of Nile tilapia juveniles Oreochromis niloticus [80]. Childress et al also demonstrated that lysine supplementation of commercial fishmeal-free diet regulated MSTN and MyoG mRNA expression and controlled myogenesis in hybrid striped bass Morone chrysops × M. saxatilis [82]. These results suggested essential amino acids plays a critical role in regulating muscle growth.…”

“…Previous studies demonstrated that Leu induced proliferation promotion through miR-27a-mediated regulation of the MSTN in C2C12 cells [76,79]. Recently, growing evidence from fish suggests that muscle growth is regulated by essential amino acids [80][81][82]. Alami-Durante et al reported dietary methionine affected the expression of genes regulating specific transition points of myogenesis and the expression of muscle structural genes and growth factors involved in satellite cell activation and muscle growth [81].…”

Dietary Supplementation With Hydroxyproline Enhances Growth Performance, Collagen Synthesis and Muscle Quality of Carassius auratus Triploid

“…Recently, some growing evidences suggest that essential amino acids play a regulatory role in fish muscle development. For instance, methionine, histidine and lysine have been proved to affect muscle hyperplasia and up-regulate myog and myod gene expression in fish ( Childress et al, 2016 ; Michelato et al, 2017 ; Alami-Durante et al, 2018 ). Hyp, as a conditionally essential amino acid, is presumed to have a similar regulating impact on fish muscle growth.…”

Modelado matemático del efecto del hidrolizado de vísceras en el desempeño productivo de trucha arcoíris (Oncorhynchus mykiss)

“…En peces juveniles, la contribución relativa de la hiperplasia y la hipertrofia al crecimiento muscular está regulada por factores nutricionales entre los que las proteínas desempeñan un papel importante, dada la importancia del depósito de proteínas en el crecimiento muscular. Diferentes estudios han subrayado la importancia no solo de la cantidad de proteínas, sino también de la calidad de las proteínas en términos de equilibrio de aminoácidos para un crecimiento muscular adecuado (Childress et al, 2016;Overturf et al, 2016y Michelato et al, 2017), haciendo relevante, la efectividad de incorporar hidrolizados como fuente proteica (Usydus y Szlinder, 2012).…”