Lysine synthesis control in Corynebacterium glutamicum RC 115 in mixed substrate (glucose-acetate) medium

Paegle, Longina; Ruklisha, Maija

doi:10.1016/s0168-1656(03)00143-3

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…Changing the substrate can force C. glutamicum to produce metabolites with different concentrations . To realize the type and concentration, production time is important for substrate adjusting and high efficiency production, especially in fermentation industry .…”

Section: Resultsmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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Corynebacterium glutamicum (C. glutamicum) is a well‐known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high‐speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum, which is fed with different culture mediums. Corynebacterium glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. In the CE system, a microliter‐scale sample reservoir was designed and applied to perform tiny volume sample injection. With the assistance of microwave, the derivatization time for amino acids with FITC was greatly shortened to 6 min. Under the optimized condition, the eight candidate amino acids of metabolites could be rapidly separated within 2 min. The whole analysis process for real samples, from sampling to determination, could be shortened to less than 10 min. The results showed that C. glutamicum could produce additional l‐lysine and l‐valine as the metabolites when fed with glucose and l‐methionine, respectively. The method proved that culture mediums used to feed C. glutamicum had great effect on the bacterium's metabolites.

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Section: Resultsmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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“…The maximum yield of 63.5 mole % observed in our analysis was mainly due to the limitation of the PEP pool, which was channeled for the active transport of glucose and only a fraction of the PEP pool could be directed to OAA. This implies that lactate or acetate in combination with glucose in the media can enhance the lysine yield as all anaplerotic reactions are active [ 24 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

Analysis of optimal phenotypic space using elementary modes as applied to Corynebacterium glutamicum

Gayen

Venkatesh

2006

BMC Bioinformatics

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Background: Quantification of the metabolic network of an organism offers insights into possible ways of developing mutant strain for better productivity of an extracellular metabolite. The first step in this quantification is the enumeration of stoichiometries of all reactions occurring in a metabolic network. The structural details of the network in combination with experimentally observed accumulation rates of external metabolites can yield flux distribution at steady state. One such methodology for quantification is the use of elementary modes, which are minimal set of enzymes connecting external metabolites. Here, we have used a linear objective function subject to elementary modes as constraint to determine the fluxes in the metabolic network of Corynebacterium glutamicum. The feasible phenotypic space was evaluated at various combinations of oxygen and ammonia uptake rates.

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“…Acetate has been described as sole and combined substrate for lysine and glutamate production (Kinoshita and Tanaka, 1972;Paegle and Ruklisha, 2003;Shiio and Miyajima, 1969). The CoA transfer from succinylCoA to acetate catalyzed by Cat in vitro may bypass the succinyl-CoA synthetase reaction in the TCA cycle in vivo with concomitant acetate activation.…”

Section: Discussionmentioning

confidence: 99%

Pathway identification combining metabolic flux and functional genomics analyses: Acetate and propionate activation by Corynebacterium glutamicum

Veit

Rittmann

Georgi

et al. 2009

Journal of Biotechnology

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Lysine synthesis control in Corynebacterium glutamicum RC 115 in mixed substrate (glucose-acetate) medium

Cited by 4 publications

References 17 publications

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

Analysis of optimal phenotypic space using elementary modes as applied to Corynebacterium glutamicum

Pathway identification combining metabolic flux and functional genomics analyses: Acetate and propionate activation by Corynebacterium glutamicum

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