Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans

Abstract

A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01T grew at 10–50 °C (optimum, 35–37 °C), pH 6–10 (optimum, pH 7) and in 0–6 % NaCl (optimum, 1–3 %). Phylogenetic analyses based on 16S rRNA and combined short- and long-read genome sequencing revealed that IRB4-01T is closely related to Lysinibacillus cavernae SYSU K30005T and Lysinibacillus boronitolerans 10aT. The cell-wall peptidoglycan type was A4α (Lys–Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. Genomic DNA G+C content was 37.4 mol%, while the digital DNA–DNA hybridization revealed 33.6 and 32.3 % relatedness to L. cavernae SYSU K30005T and L. boronitolerans 10aT, respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01T represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillus irui sp. nov. is proposed. The type strain is IRB4-01T (NCIMB 15452T=LMG 32887T). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.

Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments

“…In addition to the development of stimulating conditions for the highest possible production of biosurfactants, the identification of powerful wild-type strains is an important part of the research, which has led to several screening approaches for potent production strains and their physiological characterization, including OMICS approaches [74][75][76]. In particular, the B. subtilis strain ZH1 showed in this study demonstrated the great potential of wild-type strains for the production of biosurfactants as representatives of bioactive metabolites.…”

Comparative genomics reveals insights into the potential of Lysinibacillus irui as a plant growth promoter

“…In this study, a whole-genome comparison perspective was implemented to unveil genomic traits related to plant growth promotion in three strains (B2, B3, and B7) of L. irui isolated from P. canariensis in Portugal and the ex-type strain of L. irui , initially isolated from fermented African locust beans, in Nigeria (Akintayo et al 2023 ). Our phylogenetic analysis indicated a close relationship between B2, B3 and B7 strains and the L. irui ex-type strain, which was further confirmed by ANI and DDH values.…”

“…Members of the genus Lysinibacillus produce endospores (Akintayo et al 2023 , Ahsan and Shimizu 2021 ; Burkett-Cadena et al 2019 ). In fact, we found several genes from all sporulation stages (from chromosome replication to endospore maturation) in the four L. irui genomes.…”