LysM Proteins TaCEBiP and TaLYK5 are Involved in Immune Responses Mediated by Chitin Coreceptor TaCERK1 in Wheat

Liu, Saifei; Xiao, Muye; Fang, Anfei; Tian, Binnian; Yu, Yang; Bi, Chaowei; Ma, Dongfang; Yang, Yuheng

doi:10.1021/acs.jafc.3c02686

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…The Barley stripe mosaic virus (BSMV)‐based virus‐induced gene silencing (VIGS) assay was performed, as described previously (Liu et al ., 2023 ). Briefly, the specific fragment of TaMKP1 were selected and connected it with BSMV:γ to form a recombinant vector.…”

Section: Methodsmentioning

confidence: 99%

CRISPR‐targeted mutagenesis of mitogen‐activated protein kinase phosphatase 1 improves both immunity and yield in wheat

Liu,

Zhang,

et al. 2024

Plant Biotechnology Journal

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SummaryPlants have evolved a sophisticated immunity system for specific detection of pathogens and rapid induction of measured defences. Over‐ or constitutive activation of defences would negatively affect plant growth and development. Hence, the plant immune system is under tight positive and negative regulation. MAP kinase phosphatase1 (MKP1) has been identified as a negative regulator of plant immunity in model plant Arabidopsis. However, the molecular mechanisms by which MKP1 regulates immune signalling in wheat (Triticum aestivum) are poorly understood. In this study, we investigated the role of TaMKP1 in wheat defence against two devastating fungal pathogens and determined its subcellular localization. We demonstrated that knock‐down of TaMKP1 by CRISPR/Cas9 in wheat resulted in enhanced resistance to rust caused by Puccinia striiformis f. sp. tritici (Pst) and powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt), indicating that TaMKP1 negatively regulates disease resistance in wheat. Unexpectedly, while Tamkp1 mutant plants showed increased resistance to the two tested fungal pathogens they also had higher yield compared with wild‐type control plants without infection. Our results suggested that TaMKP1 interacts directly with dephosphorylated and activated TaMPK3/4/6, and TaMPK4 interacts directly with TaPAL. Taken together, we demonstrated TaMKP1 exert negative modulating roles in the activation of TaMPK3/4/6, which are required for MAPK‐mediated defence signalling. This facilitates our understanding of the important roles of MAP kinase phosphatases and MAPK cascades in plant immunity and production, and provides germplasm resources for breeding for high resistance and high yield.

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Section: Methodsmentioning

confidence: 99%

CRISPR‐targeted mutagenesis of mitogen‐activated protein kinase phosphatase 1 improves both immunity and yield in wheat

Liu,

Zhang,

et al. 2024

Plant Biotechnology Journal

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Chitin soil amendment triggers systemic plant disease resistance through enhanced pattern-triggered immunity

Makechemu,

Goto,

Zbinden

et al. 2024

Preprint

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Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin-induced systemic effects in plants remain unknown. In this study, we investigated the effects of soil amendment with crab chitin flakes (hereafter chitin) on pattern-triggered immunity (PTI) and systemic disease resistance in various plant species. We found that soil amendment with chitin potentiates PTI and disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in lettuce, tomato, and Arabidopsis as well as against the fungal pathogen Blumeria graminis causing powdery mildew in wheat. Using micrografting in Arabidopsis, we demonstrated that this systemic effect is dependent on active chitin perception in the roots. We also showed that induced systemic resistance (ISR) and pattern-recognition receptors (PRRs)/co-receptors, but not systemic acquired resistance (SAR), are involved in the systemic effects triggered by chitin soil amendment. This systemic effect correlated with the transcriptional up-regulation of key PTI components in distal leaves upon chitin soil amendment. Notably, chitin-triggered systemic immunity was independent of microbes present in soil or chitin flakes. Together, these findings contribute to a better understanding of chitin-triggered systemic immunity, from active chitin perception in roots to the potentiation of PTI in the leaves, ultimately priming plants to mount enhanced defense responses against pathogen attacks. Our study provides valuable insights into the molecular mechanisms of chitin soil amendment and resulting induced immunity, and highlights its potential use for sustainable crop protection strategies.

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Overexpression of plant chitin receptors in wheat confers broad‐spectrum resistance to fungal diseases

Wang,

He,

Guo

et al. 2024

The Plant Journal

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SUMMARYWheat (Triticum aestivum L.) is a globally staple crop vulnerable to various fungal diseases, significantly impacting its yield. Plant cell surface receptors play a crucial role in recognizing pathogen‐associated molecular patterns (PAMPs) and activating PAMP‐triggered immunity, boosting resistance against a wide range of plant diseases. Although the role of plant chitin receptor CERK1 in immune recognition and defense has been established in Arabidopsis and rice, its function and potential agricultural applications in enhancing resistance to crop diseases remain largely unexplored. Here, we identify and characterize TaCERK1 in Triticeae crop wheat, uncovering its involvement in chitin recognition, immune regulation, and resistance to fungal diseases. By a comparative analysis of CERK1 homologs in Arabidopsis and monocot crops, we demonstrate that AtCERK1 in Arabidopsis elicits the most robust immune response. Moreover, we show that overexpressing TaCERK1 and AtCERK1 in wheat confers resistance to multiple fungal diseases, including Fusarium head blight, stripe rust, and powdery mildew. Notably, transgenic wheat lines with moderately expressed AtCERK1 display superior disease resistance and heightened immune responses without adversely affecting growth and yield, compared to TaCERK1 overexpression transgenics. Our findings highlight the significance of plant chitin receptors across diverse plant species and suggest potential strategies for bolstering crop resistance against broad‐spectrum diseases in agricultural production through the utilization of plant immune receptors.

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LysM Proteins TaCEBiP and TaLYK5 are Involved in Immune Responses Mediated by Chitin Coreceptor TaCERK1 in Wheat

Cited by 3 publications

References 53 publications

CRISPR‐targeted mutagenesis of mitogen‐activated protein kinase phosphatase 1 improves both immunity and yield in wheat

CRISPR‐targeted mutagenesis of mitogen‐activated protein kinase phosphatase 1 improves both immunity and yield in wheat

Chitin soil amendment triggers systemic plant disease resistance through enhanced pattern-triggered immunity

Overexpression of plant chitin receptors in wheat confers broad‐spectrum resistance to fungal diseases

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