2022
DOI: 10.3390/biom12050616
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Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction

Abstract: Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of lysosomal storage diseases but also in a host of others, including common neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. New technological advances have significantly increased our abi… Show more

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Cited by 9 publications
(4 citation statements)
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“…Alternatively, intra-lysosomal loading of iron nanoparticles can confer higher enrichment rates and purity, with the caveat of introducing chemicals with potential harmful actions on the native lysosomal environment. 5 With the need for a rapid and specific method to isolate intact lysosomes, which remain suitable for further omics studies, the lysosome immunoprecipitation (LysoIP) technique was initially introduced in well-established cell lines such as epithelial HEK293T. 6 This allowed for a qualitative and quantitative description of the lysosomal compartment.…”
Section: Before You Beginmentioning
confidence: 99%
“…Alternatively, intra-lysosomal loading of iron nanoparticles can confer higher enrichment rates and purity, with the caveat of introducing chemicals with potential harmful actions on the native lysosomal environment. 5 With the need for a rapid and specific method to isolate intact lysosomes, which remain suitable for further omics studies, the lysosome immunoprecipitation (LysoIP) technique was initially introduced in well-established cell lines such as epithelial HEK293T. 6 This allowed for a qualitative and quantitative description of the lysosomal compartment.…”
Section: Before You Beginmentioning
confidence: 99%
“…Lysosomes make up only 1-3% of a cell’s volume (6), which makes it challenging to interrogate changes in their molecular content under disease conditions. Recently, the LysoTagIP approach has enabled rapid immunoprecipitation of intact lysosomes, allowing subsequent analyses of their content and leading to a better understanding of human disease (6, 7). This approach is based on exogenous expression of the lysosome-resident transmembrane TMEM192 protein containing three tandem HA epitopes at its C-terminus (LysoTag) in target cells and mouse models, allowing for rapid lysosome immunoprecipitation.…”
Section: Introductionmentioning
confidence: 99%
“…The lysosome is a critical cell organelle containing a multitude of enzymes and other proteins. [16] It regulates metabolic processes and plays an important role in cell degradation, autophagy, apoptosis and immune stress. [17,18] Some digestive enzymes, which are essential for degrading damaged organelles, nucleic acids, polysaccharides, fats and proteins, [19] maintain high catalytic activity under the weak acidic microenvironment (pH 4.5-5.5) within lysosomes.…”
Section: Introductionmentioning
confidence: 99%
“…The lysosome is a critical cell organelle containing a multitude of enzymes and other proteins [16] . It regulates metabolic processes and plays an important role in cell degradation, autophagy, apoptosis and immune stress [17,18] .…”
Section: Introductionmentioning
confidence: 99%