Lysobacter antarcticus sp. nov., an SUF-system-containing bacterium from Antarctic coastal sediment

Liu, Zuoyang; Jiang, Peiqiang; Niu, Guojiang; Wang, Wenjing; Li, Jing

doi:10.1099/ijsem.0.005250

“…Lysobacter species share some common features. For instance, they are Gram-negative, rod-shaped, and aerobic bacteria [3,4]. The major fatty acids are iso-C 15 : 0 , iso-C 16 : 0 , and summed feature 9 (iso-C 17 : 1 ω9c and/or C 16 : 0 10-methyl), and the major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol [3].…”

Section: Introductionmentioning

confidence: 99%

Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov., isolated from a pond

Jung,

Chhetri,

Kim

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

0

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Two Gram-negative bacteria, designated as strains LF1T and HM2-2T, were isolated from an artificial pond in a honey farm at Hoengseong-gun, Gangwon-do, Republic of Korea. The 16S rRNA sequence analysis results revealed that strain LF1T belonged to the genus Lysobacter and had the highest sequence similarity to Lysobacter niastensis GH41-7T (99.0 %), Lysobacter panacisoli CJ29T (98.9 %), and Lysobacter prati SYSU H10001T (98.2 %). Its growth occurred at 20–37 °C, at pH 5.0–12.0, and in the presence of 0–2% NaCl. The major fatty acids were iso-C15 : 0, iso-C16 : 0, and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C content was 67.5 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain LF1T and species of the genus Lysobacter were 79.1–84.4% and 22.0–27.5 %, respectively. The 16S rRNA sequence analysis results revealed that strain HM2-2T belonged to the genus Limnohabitans and was most closely related to Limnohabitans planktonicus II-D5T (98.9 %), Limnohabitans radicicola JUR4T (98.4%), and Limnohabitans parvus II-B4T (98.4 %). Its growth occurred at 10–35 °C, at pH 5.0–11.0, and in the presence of 0–2% NaCl. The major fatty acids were C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c). The major polar lipid was phosphatidylethanolamine. The DNA G+C content was 59.9 mol%. The ANI and dDDH values between strain HM2-2T and its closely related strains were 75.1–83.0% and 20.4–26.4 %, respectively. Phenotypic, genomic, and phylogenetic data revealed that strains LF1T and HM2-2T represent novel species in the genera Lysobacter and Limnohabitans, for which the names Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov. are proposed, respectively. The type strain of Lys. stagni is LF1T (=KACC 23251T=TBRC 17648T), and that of Lim. lacus is HM2-2T (=KACC 23250T=TBRC 17649T).

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“…Phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol are the major polar lipids [2][3][4][5][6][7][8][9]. Members of the genus Lysobacter are isolated from a wide variety of habitats, including rhizosphere of pepper, plateau soil, saline-alkaline soil, lake sediment, oil-contaminated soil, iron-mined soil, deep-sea sponge, anaerobic granules, greenhouse soils, antarctic freshwater lake, marine sediment, activated sludge, fallow farmland soil and kaolin slurry [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Members of the genus Lysobacter produce a range of extracellular enzymes and other metabolites with bacteriostatic or bactericidal activity against bacteria, fungi, oomycetes and nematodes, which have been used to develop antifungal biocontrol against plant pathogens and antibiotics against human pathogens [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Lysobacter selenitireducens sp. nov., isolated from river sediment

Mao

¹

,

Li

²

,

Guo

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, yellow-pigmented, motile, flagellated and rod-shaped bacterium, designated as 13AT, was isolated from a river sediment sample of Fuyang River in Hengshui City, Hebei Province, PR China. Strain 13AT grew at 10–37 °C (optimum, 30 °C), at pH 5.0–11.0 (optimum, pH 7.0) and at 0–7 % (w/v) NaCl concentration (optimum, 0 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 13AT belongs to the genus Lysobacter , and was most closely related to Lysobacter spongiicola DSM 21749T (97.8 %), Lysobacter concretionis DSM 16239T (97.5 %), Lysobacter daejeonensis GIM 1.690T (97.3 %) and Lysobacter arseniciresistens CGMCC 1.10752T (96.9 %). Meanwhile, the type species Lysobacter enzymogenes ATCC 29487T was selected as a reference strain (95.2 %). The genomic size of strain 13AT was 3.0 Mb and the DNA G+C content was 69.0 %. The average nucleotide identity values between strain 13AT and each of the reference type strains L. spongiicola DSM 21749T, L. concretionis DSM 16239T, L. daejeonensis GIM 1.690T, L. arseniciresistens CGMCC 1.10752T and L. enzymogenes ATCC 29487T were 75.9, 76.1, 77.7, 78.0 and 73.2 %, respectively. The digital DNA–DNA hybridization values between strain 13AT and each of the reference type strains were 21.7, 22.2, 21.9, 22.7 and 23.2 %, respectively. The average amino acid identity values between strain 13AT and each of the reference type strains were 72.5, 72.9, 72.3, 75.0 and 69.2 %, respectively. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The sole respiratory quinone was identified as ubiquinone-8. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified lipid, four unidentified phospholipids and two unidentified glycolipids. Based on the phenotypic, physiological, phylogenetic and chemotaxonomic data, strain 13AT represents a novel species of the genus Lysobacter , for which the name Lysobacter selenitireducens sp. nov. is proposed. The type strain is 13AT (=JCM 34786T=GDMCC 1.2722T).

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Lysobacter changpingensis sp. nov., a novel species of the genus Lysobacter isolated from a rhizosphere soil of strawberry in China

Niu

¹

,

Ren

²

,

Zhang

³

et al. 2023

Folia Microbiol

0

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In the present work, we characterized in detail strain CM-3-T8T, which was isolated from the rhizosphere soil of strawberries in Beijing, China, in order to elucidate its taxonomic position. Cells of strain CM-3-T8T were Gram-negative, non-spore-forming, aerobic, short rod. Growth occurred at 25–37 °C, pH 5.0–10.0, and in the presence of 0–8% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CM-3-T8T formed a stable clade with Lysobacter soli DCY21T and Lysobacter panacisoli CJ29T, with the 16S rRNA gene sequence similarities of 98.91% and 98.50%. The average nucleotide identity and digital DNA-DNA hybridization values between strain SG-8 T and the two reference type strains listed above were 76.3%, 79.6%, and 34.3%, 27%, respectively. The DNA G + C content was 68.4% (mol/mol). The major cellular fatty acids were comprised of C15:0 iso (36.15%), C17:0 iso (8.40%), and C11:0 iso 3OH (8.28%). The major quinone system was ubiquinone Q-8. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylethanolamine (PME), diphosphatidylglycerol (DPG), and aminophospholipid (APL). On the basis of phenotypic, genotypic, and phylogenetic evidence, strain CM-3-T8T (= ACCC 61714 T = JCM 34576 T) represents a new species within the genus Lysobacter, for which the name Lysobacter changpingensis sp. nov. is proposed.

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Lysobacter antarcticus sp. nov., an SUF-system-containing bacterium from Antarctic coastal sediment

Cited by 8 publications

References 79 publications

Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov., isolated from a pond

Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov., isolated from a pond

Lysobacter selenitireducens sp. nov., isolated from river sediment

Lysobacter changpingensis sp. nov., a novel species of the genus Lysobacter isolated from a rhizosphere soil of strawberry in China

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