Lysogenic Conversion of Rhizobium trifolii

Barnet, Yvonne M.; Vincent, J. M.

doi:10.1099/00221287-61-3-319

Cited by 18 publications

(3 citation statements)

References 13 publications

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“…The autoplaquephenomenon is characterized by the spontaneous appearance of plaques on the bacterial lawn without exposure to any external phage. Though, autolysis has been thoroughly investigated in Pseudomonas aeruginosa (Berk and Kazanas, 1963;Shaburova et al, 2001) it has also been described in several bacteria genera, such as Bacillus cereus (McCloy, 1958), B. subtilis (Loessner et al, 1997), Brucella abortus (Renoux and Suire, 1963), Fibrobacter succinogenes (Singh et al, 2003), Myxococcus xanthus (Wireman and Dworkin, 1977;Breyen and Dworkin, 1984), Neisseria gonorrhoeae (Campbell et al, 1985), Nodularia spumigena (Jenkins and Hayes, 2006) and Rhizobium trifolii (Barnet and Vincent, 1969).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Autoplaque Phenomenon in Bacterial Communities from Spatially Heterogeneous Environment of an Abandoned Mine

Karmakar¹,

Sharma²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Introductionmentioning

confidence: 99%

Prevalence of Autoplaque Phenomenon in Bacterial Communities from Spatially Heterogeneous Environment of an Abandoned Mine

Karmakar¹,

Sharma²

2017

Int.J.Curr.Microbiol.App.Sci

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“…Bacteria-phage interactions have other important consequences for microbial ecology. The presence in lysogenic bacteria of the genomes of temperate phages, or of virulent phages in pseudolysogeny, can have a significant phenotypic effect on the host bacteria (Barnet & Vincent 1970). Importantly, phages are a potential mechanism for the dissemination of genes within groups of related bacteria, especially if they are able to survive for long periods in the environment in the absence of hosts, or have a wide host range.…”

Section: Introductionmentioning

confidence: 99%

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Mendum

Clark

Hirsch

2001

Antonie Van Leeuwenhoek

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Two Rhizobium leguminosarum biovar viceae bacteriophages with contrasting properties were isolated from a field site in which the survival of genetically modified R. leguminosarum inoculants had been monitored for several years. Inoculant strain RSM2004 was used as the indicator for phage isolation and propagation. One phage, RL1RES, was temperate and could not replicate in any of the 42 indigenous R. leguminosarum field isolates tested although nested PCR indicated that phage sequences were present in six of the isolates. The second phage, RL2RES, was virulent, capable of generalised transduction, contained DNA with modified cytosine residues, and was capable of infecting all field isolates tested although the GM inoculant strain CT0370 was resistant. Sequence with homology to RL2RES was detected by nested PCR in six of the 42 field-isolates. These were not the same isolates that showed homology to RL1RES. The implication of these findings for the survival of rhizobial inoculants, and the ecology of phages and their host bacteria, are discussed.

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“…Barnet & Vincent (1970) suggested that the receptor site for phages 7,7cr, and 8 was associated with the somatic antigen of Rhizobium. Atkins & Hayes (1972) found alterations in lipopolysaccharide (LPS) and lipoprotein (LP) of Rhizobium trifolii mutants that adsorbed phage poorly.…”

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Lipopolysaccharide as Receptor for Rhizobium Phage 1P

Zając

Russa

Lorkiewicz

1975

Journal of General Microbiology

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INTRODUCTIONThere are only a few papers concerning the sites of phage adsorption in Rhizobium. Barnet & Vincent (1970) suggested that the receptor site for phages 7,7cr, and 8 was associated with the somatic antigen of Rhizobium. Atkins & Hayes (1972) found alterations in lipopolysaccharide (LPS) and lipoprotein (LP) of Rhizobium trifolii mutants that adsorbed phage poorly.To elucidate the attachment site of phage IP to Rhizobium, we studied cell walls and their LPS preparations as phage receptors. METHODSBacterial andphage strains. Rhizobium trifolii strains 24SM, HR, 1 5 2 4~~ and XSM were used in these experiments. Mutants HR and 15, resistant to phage IP, were derived from 2 4~~. The former was obtained by U.V. treatment, whereas the latter was a spontaneous mutant isolated by selection with phage IP. Phage IP propagated on R. trijiolii 2 4 s~ was employed in assays of receptor activity of cell walls and LPS. It does not require divalent ions for its attachment to bacteria (Staniewski, 1968).Media. Bacteria were grown in medium 4, containing (g/l glass-distilled water) : K2HP0,, 3-6; MgS04.7Hz0, 0.5; NaCl, 0.5; ferric ammonium citrate, 0.05; Casamino acids, 3.0; glucose, 10.0; pH 7-6. Bacetylation of cell walls and LPS by NaOH and NH40H. Cell walls and LPS (5 mg/ml) Preparation of cell walls, LPS and polysaccharides (PS).were treated with 0.1 M-NaOH or 2.5 M-NH,OH at 37 "C for I h. The mixture was chilled, neutralized with 0.1 M-HCl and centrifuged. The pellet was washed three times and ly ophilized. LPS oxidation with NaI04. LPS was oxidized according to a modification of the procedure of Foster, Davies & Crumpton (1958). One ml of LPS (10 mg/ml) was mixed with 0.5 ml of 2 % (w/v) NaIO, and incubated at 20 "C for 12 min. Excess NaIO, was decomposed by ethylene glycol. After 20 min, 0.5 ml of I % (w/v) NaBH, was added. Excess NaBH, was then decomposed by acetic acid (0.2 ml) and dialysed owmight against distilled water.Inactivation of phage IP. Cell walls of LPS, at a concentration of I to 50 ,ug/ml in tris-HC1 buffer pH 7.4, were mixed with an equal volume of phage IP containing 6 x IO* plaque forming units (p.f.u.). The mixture was incubated at 37 "C without shaking. At intervals, samples were removed and tested for phage survival using R. trifolii 24SM as the indicator culture. 24Y I C 90Downloaded from www.microbiologyresearch.org by

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Lysogenic Conversion of Rhizobium trifolii

Cited by 18 publications

References 13 publications

Prevalence of Autoplaque Phenomenon in Bacterial Communities from Spatially Heterogeneous Environment of an Abandoned Mine

Prevalence of Autoplaque Phenomenon in Bacterial Communities from Spatially Heterogeneous Environment of an Abandoned Mine

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Lipopolysaccharide as Receptor for Rhizobium Phage 1P

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