Lysophosphatidic acid: G-protein signalling and cellular responses

Moolenaar, Wouter H.; Kranenburg, Onno; Postma, Friso R.; Zondag, Gerben

doi:10.1016/s0955-0674(97)80059-2

Cited by 481 publications

(464 citation statements)

References 45 publications

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“…The activation of ERK and p38 MAP kinase in response to S1P was also inhibited by PTX, suggesting that both ERK and p38 MAP kinase may be located downstream of the G i \G o -proteins in S1P signalling. Recent studies suggested that G "# \G "$ -proteins and Rho, one of the small G-proteins, are also upstream regulators of cell migration [5,15,37]. Thus, G i \G o -proteins may regulate, in collaboration with G "# \G "$ -proteins and Rho, the migration of endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Among a variety of G-protein-coupled receptors, it has been suggested that a group of receptors couples with the G "# \G "$ family of Gproteins. These include thrombin, lysophosphatidic acid and probably S1P receptors [15,41]. Thus, certain types of S1P receptors expressed in endothelial cells might be one of the G "$ -protein-coupled receptors implicated in angiogenesis, although their coupling has not yet been demonstrated in this cell type.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, this agent has been shown to be a non-selective antagonist of G-protein-coupled receptors [14,15]. As shown in Figure 4 S1P-induced activation of ERK and p38 MAP kinase was competitively inhibited by suramin.…”

Section: Suramin Inhibits the S1p-induced Activation Of Erk And P38 Mmentioning

confidence: 93%

“…The cellular responses elicited by S1P have been ascribed to intracellular actions. On the other hand, S1P-induced responses are also accompanied by the stimulation of several early signalling events, which are usually regulated by cell-surface receptors [8][9][10][11][12][13][14][15][16]. These signalling events include activation of phospholipase C [8][9][10][11], an increase in cytoplasmic free Ca# + concentration [8][9][10][11][12][13]16], regulation of adenylyl cyclase [8,11,12], activation of K + channels [12] and activation of Rho [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3

Kimura

Watanabe

Sato

et al. 2000

Biochemical Journal

187

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Sphingosine 1-phosphate (S1P) stimulates thymidine incorporation (DNA synthesis), cell growth and cell migration in human aortic endothelial cells (HAECs). The extent of the S1P-induced responses are comparable to those stimulated by vascular endothelial growth factor, one of the most potent stimulators of angiogenesis. These responses to S1P were mimicked by dihydrosphingosine 1-phosphate, an S1P receptor agonist, and inhibited by pertussis toxin (PTX), an inactivator of G i \G o -proteins. S1P also induced activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAP kinase). The activation of these enzymes was inhibited again by PTX and also by suramin, a non-selective receptor antagonist. S1P-induced DNA synthesis and ERK activation were inhibited

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Suramin Inhibits the S1p-induced Activation Of Erk And P38 Mmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3

Kimura

Watanabe

Sato

et al. 2000

Biochemical Journal

187

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“…Lysophosphatidic acid (LPA), a potent signaling molecule which acts through G-protein coupled receptors, can be generated by the action of a phospholipase A 2 . It has been known that LPA is implicated in a variety of cellular functions [4], [5], including stimulation of cell proliferation and differentiation, Ca 2+ mobilization, tumor cell invasion in vivo, chemotaxis, and other functions. The regulation of PLD activity and subsequent generation of different lipid signaling molecules is therefore a very important signaling event, which may be implicated in the interactions among different signaling pathways and in achieving the signaling specificity.…”

Section: Introductionmentioning

confidence: 99%

Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition

Bin¹,

Chen²,

Chai³

et al. 2000

Cell Res

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Lysophosphatidic acid receptor genevzg-1/lpA1/edg-2 is expressed by mature oligodendrocytes during myelination in the postnatal murine brain

1998

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The growth-factor-like phospholipid lysophosphatidic acid (LPA) mediates a wide variety of biological functions. We recently reported the cloning of the first G-protein-coupled receptor for LPA, called ventricular zone gene-1 (vzg-1/lpA1/edg-2) because its embryonic central nervous system (CNS) expression is restricted to the neocortical ventricular zone (Hecht et al. [1996] J. Cell Biol. 135:1071-1083). Vzg-1 neural expression diminishes at the end of the cortical neurogenetic period, just before birth. Here, we have investigated the subsequent reappearance of vzg-1 expression in the postnatal murine brain, by using in situ hybridization and northern blot analyses. Vzg-1 expression was undetectable by in situ hybridization at birth, but reappeared in the hindbrain during the 1st postnatal week. Subsequently, expression expanded from caudal to rostral, with peak expression observed around postnatal day 18. At all postnatal ages, vzg-1 expression was concentrated in and around developing white matter tracts, and its expansion, peak, and subsequent downregulation closely paralleled the progress of myelination. Double-label in situ hybridization studies demonstrated that vzg-1-expressing cells co-expressed mRNA encoding proteolipid protein (PLP), a mature oligodendrocyte marker, but not glial fibrillary acidic protein (GFAP), an astrocyte marker. Consistent with this, vzg-1 mRNA expression was reduced by 40% in the brains of jimpy mice, which exhibit aberrant oligodendrocyte differentiation and cell death. Together with our characterization of vzg-1 during cortical neurogenesis, these data suggest distinct pre- and postnatal roles for LPA in the development of neurons and oligodendrocytes and implicate lysophospholipid signaling as a potential regulator of myelination.

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Lysophosphatidic acid: G-protein signalling and cellular responses

Cited by 481 publications

References 45 publications

Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3

Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3

Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition

Lysophosphatidic acid receptor genevzg-1/lpA1/edg-2 is expressed by mature oligodendrocytes during myelination in the postnatal murine brain

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