Lysosomal and Other Indigenous Non-plasmin Proteases in Bovine Milk

Larsen, Lotte Bach; Nielsen, Søren; Paludetti, Lizandra F.; Kelly, Alan L.

doi:10.1007/978-3-030-55482-8_3

Cited by 3 publications

(3 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apart from these, cathepsins B and S were also identified and quantified directly in the proteome. These have been detected earlier in milk, either based on immunological detection [16,35] or via ascribed peptides in the peptidome [16,35]. A somewhat surprising observation is that, in the present study, the amount of cathepsin D as determined based on its abundance in the proteome, was higher than that of plasminogen.…”

Section: Abundancies Of Different Protease Systems In Human Milkcontrasting

confidence: 41%

“…A total of 39 proteases, including 15 categorized as peptidases, were identified in at least 2/3 of the HM samples (Table 2). Among these, plasminogen, cathepsin D and kallikrein are among the more well-known proteases present in milk [18,35]. Apart from these, cathepsins B and S were also identified and quantified directly in the proteome.…”

Section: Abundancies Of Different Protease Systems In Human Milkmentioning

confidence: 99%

“…Major zymogen/enzyme systems known from HM are plasmin [24,26], cathepsin D, kallikrein and presumably other protease systems, including some of the other cathepsins, but their major milk form has not been studied in detail. In the case of plasminogen, in milk it is known to comprise both inactive plasminogen and active plasmin, the propeptide and the activation peptide thus need to be removed for activation [34], while procathepsin D is activated through the removal of an N-terminal activation peptide by a combination of autoactivation at an acidic pH and other proteases [35,36].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk

Thesbjerg,

Nielsen,

Sundekilde

et al. 2023

Nutrients

View full text Add to dashboard Cite

The presence of proteases and their resulting level of activity on human milk (HM) proteins may aid in the generation of indigenous peptides as part of a pre-digestion process, of which some have potential bioactivity for the infant. The present study investigated the relative abundance of indigenous peptides and their cleavage products in relation to the abundance of observed proteases and protease inhibitors. The proteomes and peptidomes in twelve HM samples, representing six donors at lactation months 1 and 3, were profiled. In the proteome, 39 proteases and 29 protease inhibitors were identified in 2/3 of the samples. Cathepsin D was found to be present in higher abundance in the proteome compared with plasmin, while peptides originating from plasmin cleavage were more abundant than peptides from cathepsin D cleavage. As both proteases are present as a system of pro- and active- forms, their activation indexes were calculated. Plasmin was more active in lactation month 3 than month 1, which correlated with the total relative abundance of the cleavage product ascribed to plasmin. By searching the identified indigenous peptides in the milk bioactive peptide database, 283 peptides were ascribed to 10 groups of bioactivities. Antimicrobial peptides were significantly more abundant in month 1 than month 3; this group comprised 103 peptides, originating from the β-CN C-terminal region.

show abstract

Section: Abundancies Of Different Protease Systems In Human Milkcontrasting

confidence: 41%

Section: Abundancies Of Different Protease Systems In Human Milkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk

Thesbjerg,

Nielsen,

Sundekilde

et al. 2023

Nutrients

View full text Add to dashboard Cite

show abstract

Activities of indigenous proteases in cow, buffalo and goat milk of Indian subcontinent and their correlation with somatic cell count

Gautam

Sharma

Atbhaiya

et al. 2023

International Dairy Journal

View full text Add to dashboard Cite

From lab‐based to in‐line: Analytical tools for the characterization of whey protein denaturation and aggregation–A review

Finnegan,

Goulding,

O'Callaghan

et al. 2024

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Whey protein denaturation and aggregation have long been areas of research interest to the dairy industry, having significant implications for process performance and final product functionality and quality. As such, a significant number of analytical techniques have been developed or adapted to assess and characterize levels of whey protein denaturation and aggregation, to either maximize processing efficiency or create products with enhanced functionality (both technological and biological). This review aims to collate and critique these approaches based on their analytical principles and outline their application for the assessment of denaturation and aggregation. This review also provides insights into recent developments in process analytical technologies relating to whey protein denaturation and aggregation, whereby some of the analytical methods have been adapted to enable measurements in‐line. Developments in this area will enable more live, in‐process data to be generated, which will subsequently allow more adaptive processing, enabling improved product quality and processing efficiency. Along with the applicability of these techniques for the assessment of whey protein denaturation and aggregation, limitations are also presented to help assess the suitability of each analytical technique for specific areas of interest.

show abstract

Lysosomal and Other Indigenous Non-plasmin Proteases in Bovine Milk

Cited by 3 publications

References 98 publications

Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk

Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk

Activities of indigenous proteases in cow, buffalo and goat milk of Indian subcontinent and their correlation with somatic cell count

From lab‐based to in‐line: Analytical tools for the characterization of whey protein denaturation and aggregation–A review

Contact Info

Product

Resources

About