Lysosomal responses to heat-shock of seasonal temperature extremes in Cd-exposed mussels

Múgica, M.; Izagirre, Urtzi; Marigómez, Ionan

doi:10.1016/j.aquatox.2015.04.020

Cited by 17 publications

(16 citation statements)

References 59 publications

(107 reference statements)

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“…However, when these conditions are not met, the increase in temperature may negatively affect shellfish. Changes in mussels behaviour, immune response, standard metabolic rate, reduction of energy available for the somatic metabolism, and negative impacts on growth rates have been associated with increasing temperature (Anestis et al, 2007;Coppola et al, 2017;Múgica et al, 2015). Increase of temperature may also promote the uptake, accumulation and metabolization of some pollutants, like metals (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Increase of temperature may also promote the uptake, accumulation and metabolization of some pollutants, like metals (e.g. Ni and Cd) and radionuclides, as well as persistent organic pollutants (POPs) and methylmercury (Alava et al, 2017;Banni et al, 2014;Coppola et al, 2017;Dallas et al, 2016;Múgica et al, 2015;Sokolova and Lannig, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Combined effects of warming and acidification on accumulation and elimination dynamics of paralytic shellfish toxins in mussels Mytilus galloprovincialis

Braga

Marques

Gago-Martı́nez

et al. 2018

Environmental Research

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Harmful algal blooms (HAB) have been increasing in frequency and intensity most likely due to changes on global conditions, which constitute a significant threat to wild shellfish and its commercial farming. This study evaluated the impact of increasing seawater temperature and acidification on the accumulation/elimination dynamics of HAB-toxins in shellfish. Mytilus galloprovincialis were acclimated to four environmental conditions simulating different climate change scenarios: i) current conditions, ii) warming, iii) acidification and iv) interaction of warming with acidification. Once acclimated, mussels were exposed to the paralytic shellfish toxins (PSTs) producing dinoflagellate Gymnodinium catenatum for 5 days and to non-toxic diet during the subsequent 10 days. High toxicity levels (1493 µg STX eq. kg) exceeding the safety limits were determined under current conditions at the end of the uptake period. Significantly lower PSP toxicity levels were registered for warming- and acidification-acclimated mussels (661 and 761 µg STX eq. kg). The combined effect of both warming and acidification resulted in PSP toxicity values slightly higher (856 μg STX eq. kg). A rapid decrease of toxicity was observed in mussels at the current conditions after shifting to a non-toxic diet, which was not noticed under the predicted climate change scenarios. Variability of each PST analogue, measured throughout the experiment, highlighted different mechanisms are associated with changes of each environmental factor, although both resulting in lower toxicity. Warming-acclimated mussels showed lower accumulation/elimination rates, while acidification-acclimated mussels showed higher capability to accumulate toxins, but also a higher elimination rate preventing high toxicity levels. As different mechanisms are triggered by warming and acidification, their combined effect not leads to a synergism of their individual effects. The present work is the first assessing the combined effect of climate change drivers on accumulation/elimination of PSTs, in mussels, indicating that warming and acidification may lead to lower toxicity values but longer toxic episodes. PSTs are responsible for the food poisoning syndrome, paralytic shellfish poisoning (PSP) in humans. This study can be considered as the first step to build models for predicting shellfish toxicity under climate change scenarios.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined effects of warming and acidification on accumulation and elimination dynamics of paralytic shellfish toxins in mussels Mytilus galloprovincialis

Braga

Marques

Gago-Martı́nez

et al. 2018

Environmental Research

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“…It was subsequently diluted in FSW to obtain a working solution with a final concentration of 0.2 mmol l 21 . This concentration, although higher than those found in the environment, was effective in inducing oxidative stress in the hemocytes of Botryllus schlosseri (Franchi and Ballarin, 2013), and was within the concentration ranges used in toxicological experiments with other aquatic organisms (Jeppe et al, 2014;Koutsogiannaki et al, 2015;Mùgica et al, 2015).…”

Section: Exposure To Cadmiummentioning

confidence: 67%

Protection from Oxidative Stress in Immunocytes of the Colonial Ascidian Botryllus schlosseri: Transcript Characterization and Expression Studies

Franchi

Ballin

Ballarin

2017

The Biological Bulletin

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Botryllus schlosseri is a cosmopolitan colonial ascidian that undergoes cyclical generation changes, or take-overs, during which adult zooids are resorbed and replaced by their buds. At take-over, adult tissues undergo diffuse apoptosis and effete cells are massively ingested by circulating phagocytes, with a consequent increase in oxygen consumption and in production of reactive oxygen species (ROS). The latter are responsible for the death of phagocytes involved in the clearance of apoptotic cells and corpses by phagocytosis-induced apoptosis. However, the majority of phagocytes and hemocytes do not die, even if they experience oxidative stress. This fact suggests the presence of detoxification mechanisms assuring their protection. To test this assumption, we searched for transcripts of genes involved in detoxification in the transcriptome of B. schlosseri. We identified and characterized transcripts for Cu/Zn superoxide dismutase (SOD), γ-glutamyl-cysteine ligase modulatory subunit (GCLM), glutathione synthase (GS), and two glutathione peroxidases (i.e., GPx3 and GPx5), all involved in protection from ROS. We also carried out a phylogenetic analysis of the putative amino acid sequences, confirming their similarity to their vertebrate counterparts, and studied the location of their mRNAs by in situ hybridization on hemocyte monolayers. We also analyzed gene transcription during the colonial blastogenetic cycle, which is the interval of time between one take-over and the next, by qRT-PCR. In addition, we investigated the effects of cadmium (Cd), an inducer of oxidative stress, on gene transcription. Our results indicated that i) antioxidant gene expression is modulated in the course of the blastogenetic cycle and upon exposure to Cd, and ii) hemocytes synthesize both enzymatic and nonenzymatic antioxidants, in line with the idea that they represent a major detoxification system for ascidians.

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“…Temperature has a key impact on the physiology of poikilothermic organisms, influencing food acquisition, metabolic rates, growth, reproduction, and survival, among other biological processes (Dong, Miller, Sanders, & Somero, 2008;Resgalla, Brasil, & Salomão, 2007). For example, heat-shock events can compromise the growth and reproduction of mussels when increased energetic demands are not fulfilled despite the concomitant increase in filtration rates (Ferreira-Rodríguez & Pardo, 2017;Múgica, Izagirre, & Marigómez, 2015). In this sense, the occurrence of heat waves is a potential threat for macroinvertebrates, especially those from freshwater environments (Sousa, Ilarri, Souza, Antunes, & Guilhermino, 2011).…”

mentioning

confidence: 99%

“…Previous studies have shown a size and temperature dependence of CR in molluscs, including the invasive non-native bivalves Corbicula fluminea (Lauritsen, 1986;Müller, 1774), Dreissena polymorpha Pallas, 1771 (Aldridge, Payne, & Miller, 1995), and Limnoperna fortunei Dunker, 1857 (Sylvester, Dorado, Boltovskoy, Juárez, & Cataldo, 2005). At the cellular level, lysosomal responses are used worldwide as biomarkers for evaluating environmental pollution (Cajaraville, Abascal, Etxeberria, & Marigómez, 1995) and other environmental stressors, such as the effect of temperature alone or in combination with other stressors (Múgica et al, 2015;Zhang, Li, Vandepeer, & Zhao, 2006). Exposure to extreme temperatures often results in oxidative stress, causing metabolic waste that digestive cell lysosomes help to expel (Abele, Burlando, Viarengo, & Portner, 1998;Moore, Allen, & McVeight, 2006).…”

mentioning

confidence: 99%

Multibiomarker response shows how native and non‐native freshwater bivalves differentially cope with heat‐wave events

Ferreira‐Rodríguez

Fernández

Cancela

et al. 2018

Aquatic Conservation

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Temperature has a key impact on the physiology, food acquisition, metabolic rate, growth, and reproduction of organisms. Thus, exploring the effects of climate change on species physiology is important for the conservation and management of native and non‐native bivalve species. In this work, an integrative laboratory approach was used to compare the physiological, cellular, and molecular responses of the native Unio delphinus and the invasive non‐native Corbicula fluminea under a natural heat‐wave event. Whereas the filtration rate in C. fluminea was clearly affected throughout the whole heat‐wave event (from the heat peak onwards), it was only significantly affected during the heat‐wave climax in U. delphinus, and recovered afterwards. At the cellular level, lysosomal membrane stability was affected during the heat‐wave climax, but recovered afterwards, in both native and non‐native bivalve species. At the molecular level, hsp70 and hsp90 gene expression were increased and decreased (respectively) in C. fluminea after the heat wave. In contrast, only hsp90 gene expression decreased in U. delphinus during the climax and after the heat wave. The present work demonstrates that accurate monitoring of native and non‐native species using an integrated biomarker approach provides new insights into how both species might cope with the expected increase in frequency and intensity of heat events in a global warming context. Direct and indirect impacts of heat waves have been associated with massive mortalities of both native and non‐native species. Despite present results showing that C. fluminea are more sensitive to heat waves than U. delphinus, massive mortality events might act in favour of C. fluminea as it has a higher reproductive capacity. Although native freshwater mussel species conservation is already threatened by overexploitation, flow regulation and diversions, habitat destruction, and pollution, it is probably also adversely affected by global warming and the presence of invasive non‐native species, such as C. fluminea.

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Lysosomal responses to heat-shock of seasonal temperature extremes in Cd-exposed mussels

Cited by 17 publications

References 59 publications

Combined effects of warming and acidification on accumulation and elimination dynamics of paralytic shellfish toxins in mussels Mytilus galloprovincialis

Combined effects of warming and acidification on accumulation and elimination dynamics of paralytic shellfish toxins in mussels Mytilus galloprovincialis

Protection from Oxidative Stress in Immunocytes of the Colonial Ascidian Botryllus schlosseri: Transcript Characterization and Expression Studies

Multibiomarker response shows how native and non‐native freshwater bivalves differentially cope with heat‐wave events

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