Lysosomes and the "toxicity" of Rickettsials. II. Non-cytocidal interactions of egg-grown <i>C</i>. <i>psittaci</i> 6BC and in vitro macrophages

Kordová, Nonna; Poffenroth, Linda; Wilt, J. C.

doi:10.1139/m72-134

Cited by 9 publications

(16 citation statements)

References 19 publications

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“…During the course of in vitro cultivation of noninfected controls (in 10% inactivated calf serum) which lasted for periods of 1-6 days, there was no evidence of injury of the monocytes as determined by supravital staining with trypan blue, neutral red, and for acid phosphatase; this was in agreement with our earlier studies (14,16). Normal peritoneal macrophages from mice obtained without an inducer of exudate were shown to have characteristics which are typical of the peritoneal macrophages of many species (1 9) and are seen in Fig.…”

Section: Noninfecred Controlssupporting

confidence: 92%

“…When high, "toxic" doses of the agent were applied, the host cells were killed by release of lysosomal enzymes before the infecting agent had an opportunity to multiply (14,15). These events were in marked contrast to the effect of non-cytopathic egg-grown C. psittaci 6BC strains, where lysosomes retained their integrity and host cells were not damaged (16,17).…”

Section: Introductionmentioning

confidence: 99%

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Lysosomes and the "toxicity" of Rickettsiales. IV. Ultrastructural studies of macrophages infected with a cytopathic L cell-grown C. psittaci 6BC strain

Kordová¹,

Hoogstraten²,

Wilt³

1973

Can. J. Microbiol.

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KORDOVA, N., J. HOOGSTRATEN, and J. C. WILT. 1973. Lysosomes and the "toxicity" of Rickettsiales.IV. Ultrastructural studies of macrophages infected with a cytopathic L cell-grown C. psittaci 6BC strain. Can. J. Microbiol. 19: 315-320. Mouse macrophages in vitro inoculated with a low dose of a cytopathic L cell-grown C. psittaci 6BC strain were harvested at times when a delayed cytopathic effect (CPE) was observed by light microscopy and when a cytochemical test for acid phosphatase revealed lysosomal enzyme release. An array of different lysosomal types was revealed by ultrastructural studies such as digestive vesicles with single o r double outer membranes and whorls of membranes, dense residual bodies, aggregated and single scattered ferritin-like granules, and fragments of membranes. Enlargement of cysternae of endoplasmic reticulum, disintegration of cytoplasmic organelles, and thinning of ground cytoplasm were also observed. Cytoplasmic degeneration has been observed both within membrane-limited structures and in wider areas not limited by recognizable membranes.A high concentration of the agent resulted in an early toxic effect; this was accompanied by a rapid vacuolization of the cytoplasm of the macrophages which showed a poorly developed ultrastructure, and absence of mitochondria and cytoplasmic organelles. Cytochemical tests indicated that these alterations correlated with release of lysosomal acid phosphatase and disintegration of neutral red stained granules. Ultrastructural studies support the view that disintegration of lysosomes may be considered a n important contributory factor in the pathology of chlamydia1 infection.

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Section: Noninfecred Controlssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Lysosomes and the "toxicity" of Rickettsiales. IV. Ultrastructural studies of macrophages infected with a cytopathic L cell-grown C. psittaci 6BC strain

Kordová¹,

Hoogstraten²,

Wilt³

1973

Can. J. Microbiol.

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“…It seemed of interest to examine the behavior of the goose isolates at the cellular level, in indicator host systems which we have used to differentiate cytopathic from non-cytopathic variant strains of C. ysittaci 6BC (18)(19)(20)(21). For this purpose, the partially purified inoculum obtained after three subpassages of the goose egg isolates (from dead goose embryos) in the yolk sac of chicken embryos was used, and was assayed in cultured peritoneal mouse macrophages as well as in L cells.…”

Section: Resultsmentioning

confidence: 99%

“…elsewhere (18,20). Briefly, the agent was collected from cultivation, whereas large forms of the agent chicken yolk sacs (third subpassage), partially purified by were predominant in the earlier days of cultivadifferential centrifugation, resuspended in growth medium containing 200 pg streptomycin sulfate per milliliter, and (compare Fig.…”

Section: Response Of Itz Vitro Macrophagesmentioning

confidence: 99%

Preliminary characterization of a chlamydial agent isolated from embryonated snow goose eggs in northern Canada

Wilt¹,

Kordová²,

Wilt³

1972

Can. J. Microbiol.

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Chlamydiae-like, streptomycin-resistant parasites have been isolated from a high percentage of embryonated snow goose eggs collected at various stages of incubation; the agents stained specifically by immunofluorescence with antiserum against psittacosis and multiplied intensively in the yolk sacs of chicken embryos and induced in mice the production of antibodies that reacted in complement-fixing (CF) tests with a psittacosis group antigen. The isolates showed a high infectivity, but a low pathogenicity for chick embryos and mice. High doses of the isolate produced no cytopathic effect (CPE) in cultured mouse peritoneal macrophages and L cells. Release of lysosomal acid phosphatase did not occur in the inoculated macrophages throughout the time of observation.

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“…Bose lysosomal stability of C . psittaci 6BC-infected mouse macrophages (Kordova et al, 1972a) and L cells (Kordova et al, 1972b) …”

Section: Introductionmentioning

confidence: 99%

Extensive heterogeneity of the protein composition of Chlamydia trachomatis following serial passage in two different cell lines

Goswami

Vretou²,

Bose³

1990

Journal of General Microbiology

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To determine if the host-modulated adherence characteristics of the intracellular bacterial pathogen Chlamydia fruhomatis were due to the acquisition of altered surface-exposed proteins, highly purified chlamydiae grown in two different host cells were analysed. Two serovars, L1 and E, were grown for multiple passages in both HeLa and McCoy host cells. Numerous protein differences in the chlamydial elementary bodies (EB) of each serovar grown in the two different hosts were detected by two-dimensional (2-D) gel electrophoresis and fluorography of radioactively labelled proteins. At least four to six serial passages in the alternative host were necessary before the changes were apparent. Iodination of suspensions of purified chlamydiae and 2-D electrophoresis revealed several surface proteins that were determined by the host cells in which the bacteria had replicated. These iodinated chlamydial proteins were removed by treatment of the iodinated EB with trypsin, indicating their location at the bacterial surface. Two of the major constituents of the outer-membrane complex, the cysteine-and methionine-rich 60 kDa and 40 kDa proteins, remained unchanged in both molecular mass and charge during the host adaptation. Several chlamydial proteins capable of binding iodinated host membrane preparations also exhibited hostdependent alterations. Immunoblotting experiments with a rabbit and a human polyclonal sera indicated that distinct host-specified chlamydial proteins were reactive with the two sera.

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Lysosomes and the "toxicity" of Rickettsials. II. Non-cytocidal interactions of egg-grown C. psittaci 6BC and in vitro macrophages

Cited by 9 publications

References 19 publications

Lysosomes and the "toxicity" of Rickettsiales. IV. Ultrastructural studies of macrophages infected with a cytopathic L cell-grown C. psittaci 6BC strain

Lysosomes and the "toxicity" of Rickettsiales. IV. Ultrastructural studies of macrophages infected with a cytopathic L cell-grown C. psittaci 6BC strain

Preliminary characterization of a chlamydial agent isolated from embryonated snow goose eggs in northern Canada

Extensive heterogeneity of the protein composition of Chlamydia trachomatis following serial passage in two different cell lines

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