Lysozyme and chitinase in blood and lymphomyeloid tissues of marine fish

Occurrence of lysozyme in the eggs of coho salmon Oncorhynchus kisutch

“…Lysozyme has also been -20°C. Prior to use, one group of eggs was thawed, detected in tissues of different marine fishes (Sankaran homogenized in a tissue grinder at room temperature, & Gurnani 1972, Fange et al 1976 White and the homogenate filtered through cotton cheese-1976), and recently, it was reported to occur in the cloth. Another group of thawed eggs was transferred kidney and digestive tissues of rainbow trout onto absorbent pads of filter paper in a petri dish, to Oncorhynchus mykiss (Lindsay 1986, Grinde et al drain the coelornic fluid, and the yolk (EY) was 1988) and in the kidney tissues of Atlantic salmon removed using a capillary tube.…”

“…However, the presence of genate (EH) and the EY were assayed for lysozyme the enzyme in the eggs of fish has never been reported. activity by the lysoplate method (Fange et al 1976, This is an important question because the presence McHenery et al 1979). of lysozyrne in salrnonid eggs could help to explain Lysozyme purification.…”

“…The agarose contained 0.06M phosphate buffer pH 6.0, 0.02M NaC1, and Micrococcus lysodeikticus (0.6 mg freeze-dried cells ml-l, Sigma). After incubation for 20 h at room temperature in a humid chamber, the diameters of zones of M. lysodeikticus lysis were measured and compared to those produced by hen egg-w-hte iysozyme (HEWL, Sigma) standards (50 to 2000 pg ml-l) (Fange et al 1976, McHenery et al 1979.…”

Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

“…Chitinase activity is present in the lymphomyeloid tissue of the Chimaera monstrosa, in the esophagus and lymphomyeloidepigonal organs of Etmopterus spinax and Raja pulchra [13], and in the stomach, liver, and spleen of Mustelus manazo [24]. These findings indicated that chitinase was found in most rays and sharks, where it participates in physiological roles.…”

Antibacterial Proteins and Peptides in Nurse Shark (<em>Ginglymostoma Cirratum</em>) Peripheral Blood Leukocytes

“…Other lysozymes have been discovered, and they differ in primary structure, physicochemical and immunological criteria (Jolles 1969;Jolles et al 1984;Jolles 1996). These other types of lysozyme have been found in bacteria, bacteriophages, plants, the invertebrate sea star, Asterias rubens, and some teleosts (Tsugita et al 1968;Jolles et al 1975;Fange 1976). There has been lysozyme-like activity detected in an elasmobranch, using nurse shark serum, however, this activity was not studied in detail (Hedayati 1992).…”