Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces

Subramanian, Palaniappan; Leśniewski, Adam; Kamińska, Izabela; Vlandas, Alexis; Vasilescu, Alina; Niedziółka‐Jönsson, Joanna; Pichonat, E.; Happy, H.; Boukherroub, Rabah; Szunerits, Sabine

doi:10.1016/j.bios.2013.06.026

Cited by 127 publications

(93 citation statements)

References 36 publications

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“…Next to these approaches, in recent years, a few aptamer-based analytical methods have been developed towards lysozyme recognition and detection [47,[55][56][57][58][59][60][61][62][63]. Aptamers are small single-stranded oligonucleotides (DNA or RNA) that are selected in vitro to bind with high affinity and specificity any selected target of choice, from small-sized, such as metal ions [64], to large ones, such as cells [65].…”

Section: Quantification Methodsmentioning

confidence: 99%

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

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Section: Quantification Methodsmentioning

confidence: 99%

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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“…A reduction process in principle occurs on the cathode [58]. However, as seen in the example above, the 'reduction' of GO has also been observed to occur on the anode surface during anodic EPD processes, leading to production of reduced GO films [9,40,49,79,136,137]. The extent of reduction does not depend on deposition time [49], but is mainly controlled by voltage.…”

Section: Epd-based Reduction Mechanism Of Gomentioning

confidence: 98%

Electrophoretic deposition of graphene-related materials: A review of the fundamentals

Diba

Fam

Boccaccını

et al. 2016

Progress in Materials Science

228

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The Electrophoretic Deposition (EPD) of graphene-related materials (GRM) is an attractive strategy for a wide range of applications. This review paper provides an overview of the fundamentals and specific technical aspects of this approach, highlighting its advantages and limitations. Since obtaining a stable dispersion of charged particles is a pre-requisite for successful EPD, the strategies for suspending GRM in different media are discussed, along with the resulting influence on the deposited film. Most importantly, the kinetics involved in the EPD of GRMs and the factors that cause deviation from linearity in Hamaker's Law are reviewed. Side reactions often influence both efficiency and the nature of the deposited material; examples include the reduction of graphene oxide (GO) and related materials, as well as the decomposition of the suspension medium at high potentials. The microstructural characteristics of GRM deposits, and their degree of reduction, strongly influence their performance in their intended function. These factors will also determine, to a large extent, the commercial potential of this technique for applications involving GRM, and are therefore discussed here.

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“…However, too many chemical treatments and long processing time employed for chemical grafting method make it less environmentally friendly, taken together with the difficulties in processing large panels and the practical application. Due to process simplicity, uniformity of the deposited films, and good control of the deposited thickness, EPD is known to be one of the most promising manipulation techniques to produce large-scale reinforcement of nanoparticles in composite applications, which was proposed to avoid these problems and has been successfully applied to deposit GO on CF surface with strong covalent binding [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Effects of electrophoretically deposited graphene oxide coatings on interfacial properties of carbon fiber composite

et al. 2015

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Graphene oxide (GO) was utilized to improve the interfacial performance of carbon fiber (CF) and epoxy resin. In this work, a simple and an effective way, without complex chemical reactions and long processing time, is proposed to prepare GO/CF hybrid fiber via electrophoretic deposition (EPD). In this method, ultrasonically assisted EPD is employed to deposit GO on CF when oxidized carbon fibers are continuously immersed into GO aqueous dispersion. Surface functional group, surface roughness, and surface morphologies of carbon fibers were examined by Fourier transform infrared spectrometer, atomic force microscopy, and scanning electron microscopy, respectively. Surface free energy of the fiber was characterized by dynamic contact angle analysis. Results show that uniform GO coating with rough morphology can be formed on carbon fibers. Moreover, surface wettability of carbon fibers to epoxy resin is significantly improved by enhanced surface free energy due to the deposition of GO. Meanwhile, the introduction of GO in the carbon fiber/epoxy resin composites results in a 56.1 % enhancement in the interfacial shear strength which confirms the remarkable improvement in the interfacial adhesion strength of the composites. The interfacial fracture mechanism was also analyzed.

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Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces

Cited by 127 publications

References 36 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

Electrophoretic deposition of graphene-related materials: A review of the fundamentals

Effects of electrophoretically deposited graphene oxide coatings on interfacial properties of carbon fiber composite

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