Lysozyme, lactoferrin, and secretory immunoglobulin A content in breast milk: influence of duration of lactation, nutrition status, prolactin status, and parity of mother

Hennart, Philippe; Brasseur, Daniel; Delogne-Desnoeck, J.; Dramaix, M; Robyn, Claude

doi:10.1093/ajcn/53.1.32

Cited by 149 publications

(108 citation statements)

References 29 publications

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“…The molecular weight of the protein band corresponding to DM lactoferrin was about 80.0 kDa. The quantitative determination of DM lactoferrin ( Figure 2A) gave a result of 0.080 ±0.0035 g/L, similar to that found in mare (0.1 g/L),cow (0.02-0.2 g/L), goat (0.06-0.40 g/L), and sheep milk (0.135 g/L) [20,21], but lower when compared with the lactoferrin content in human milk (up to 1.0-6.0 g/L) [22].…”

Section: Characterization Of Donkey Milk Antimicrobial Factors: Lactosupporting

confidence: 75%

A Proteomic Study on Donkey Milk

Vincenzetti¹,

Amici²

2012

Biochem Anal Biochem

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In children with Cow Milk Protein Allergy (CMPA), when it is not possible to breast feed or to use cow milk, the clinical use of donkey milk is considered since several studies have demonstrated the high similarity of donkey milk compared to human milk.An analysis was performed on donkey milk protein profile by two-dimensional electrophoresis (2-DE) followed by N-terminal sequencing in order to give a panoramic view of the proteins that are present in donkey milk. Furthermore, the interest was focused on the casein fractions and on their phosphorylation degree that may influence the calcium binding ability of caseins. At this purpose experiments on donkey milk casein dephosphorylation have been performed and the dephosphorylated casein fractions have been identified after 2-DE analysis followed by N-terminal sequencing. Among caseins were found mainly αs1-and β-caseins that showed a considerable heterogeneity due to variable degree of phosphorylation and to the presence of genetic variants. Finally, a quantitative determination of some antimicrobial proteins, such as lactoferrin and lactoperoxidase, that could be able to stimulate the development of the neonatal intestine, was performed in donkey milk, with the results being 0.080±0.0035 g/L and 0.11±0.027 mg/L, respectively. From the obtained data is evinced that human and donkey milk contain considerable amounts of lysozyme and lactoferrin but lactoperoxidase is present only in small amounts, confirming the high similarity between donkey and human milk. The present study on donkey milk proteins may be useful to assess the nutritional characteristics of this milk that is used to feed children affected by CMPA, but also may open the possibility of utilizing donkey milk in the general population to benefit subjects with CMPA, such as adults and the elderly.

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Section: Characterization Of Donkey Milk Antimicrobial Factors: Lactosupporting

confidence: 75%

A Proteomic Study on Donkey Milk

Vincenzetti¹,

Amici²

2012

Biochem Anal Biochem

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“…Recovery studies showed close agreement between the theoretical and measured protein concentrations in dilution-overloadind assays (linear recovery on large ranges of concentrations with mean percentage very close to 100%). Results of the measurements of nine proteins performed together in a great number of human mature milk samples are, for the most part, in good agreement with those previously reported in past years (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), using different techniques and, the most often, restricted to the determination of the one or another of these proteins. Emergence of new analytical procedures in the late years, such as conventional or microparticleenhanced immunonephelometry, today allows major proteins of human milk to be analyzed with sensitivity, easiness, and singleness of methodologies.…”

Section: Discussionsupporting

confidence: 89%

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Montagne¹,

Trégoat²,

Cuillière³

et al. 2000

Clinical Biochemistry

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Objectives: Microparticle-enhanced nephelometric immunoassays for six human milk proteins (␤-casein, -casein, ␣-lactalbumin, serum albumin, lactoferrin, and lysozyme) and conventional immunonephelometry assays for immunoglobulin A, C3, and C4 complement proteins were developed and characterized. Design and methods: Microparticle-enhanced nephelometric immunoassays are competitive assays based on the nephelometric quantification of the inhibition of microparticle-protein conjugates immunoagglutination by the proteins to be assayed. Results: High precision (CVs ranged from 1% to 14% in within-and between-assays) and recovery (linear recovery in dilution-overloading assay) ensure a reliable determination of the main human milk proteins by single-step homogeneous nephelometric immunoassays, accurate over wide ranges of concentration. These immunoassays were easily applied to a large number of mature human milk samples (between 373 and 503 according to the proteins tested). Conclusions:The immunoassays developed could be applied to the fast determination of human milk protein profile usable for nursery milk bank and fortification.

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“…There is some discrepancy between our results and other reports perhaps due to differences in the time of breast milk collection and studies not taking into account the degree of prematurity, parity and mode of delivery, variables that may significantly affect the bioactive protein levels. 3,11,15,25 In conclusion, transitional milk after term as well as preterm delivery is superior to mature milk in terms of the content of the bioactive proteins. Breast milk from mothers who deliver at less than 32 weeks of gestation is a good source of factors that have antibacterial and anti-inflammatory activities but is constantly deficient in leptin, which is involved in the regulation of the neuroendocrine system.…”

Section: Discussionmentioning

confidence: 97%

“…4,24 However, the data in the medical literature is inconsistent and some studies have shown no effect of nutrition and/or adiposity on the milk content of lactoferrin, lysozyme, sIgA and leptin. 25,26 We used a smaller sample of mothers who delivered at term but the data was collected consistently (four times) during the first month of lactation and found to be normally distributed. There is some discrepancy between our results and other reports perhaps due to differences in the time of breast milk collection and studies not taking into account the degree of prematurity, parity and mode of delivery, variables that may significantly affect the bioactive protein levels.…”

Section: Discussionmentioning

confidence: 99%

Biologically active breast milk proteins in association with very preterm delivery and stage of lactation

Mehta

Petrová

2010

J Perinatol

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Objective: The aim of this study was to identify the independent effect of very preterm gestation on breast milk content of biologically active proteins (secretory immunoglobulin A (sIgA), lysozyme, lactoferrin, osteoprotegerin (OPG), leptin, adiponectin and b-endorphin (b-EP)) during the first month of lactation.Study Design: We collected samples of transitional (6 to 8 and 13 to 15 days) and mature (20 to 22 and 27 to 29 days) milk from mothers after term (38 to 41 weeks) or very preterm (24 to 31 weeks) delivery. The levels of sIgA, lysozyme, lactoferrin, OPG, leptin, adiponectin and b-EP in the breast milk were quantified using enzyme-linked immunosorbent assay or enzyme immunoassay kits. Statistical analysis included descriptive statistics and regression analysis.Result: Sixty breast milk samples were collected from 15 mothers after very preterm (preterm breast milk, PBM) and 20 samples from 5 mothers after term (term breast milk, TBM) deliveries. Decrease in lysozyme, lactoferrin, OPG, leptin, adiponectin and b-EP but no change in sIgA was recorded during the first month of lactation in both TBM and PBM. The IgA, lysozyme and adiponectin were higher in PBM than in TBM, whereas concentrations of lactoferrin, OPG and leptin were higher in TBM than in PBM (P<0.05 to 0.0001). A similar pattern was seen in the lysozyme, leptin and adiponectin concentration in mature milk. Increased b-EP levels in breast milk were associated with the vaginal mode of delivery but not gestational age. Conclusion:Although a similar pattern of change was observed in the breast milk bioactive proteins during the first month of lactation after term and very preterm gestation, PBM is a better source of factors with antibacterial/anti-inflammatory activities but is constantly deficient in leptin, which is involved in neuroendocrine regulation.

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Lysozyme, lactoferrin, and secretory immunoglobulin A content in breast milk: influence of duration of lactation, nutrition status, prolactin status, and parity of mother

Cited by 149 publications

References 29 publications

A Proteomic Study on Donkey Milk

A Proteomic Study on Donkey Milk

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Biologically active breast milk proteins in association with very preterm delivery and stage of lactation

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