Lysozyme release from isolate pea protein and starch based films and their antimicrobial properties

Starch-protein active films for food preservation

“…In addition, the high molecular weight of the antimicrobial agents could disable them to diffuse into the food model, coherently with the results obtained by Fabra et al (2014) for LZ incorporated into both starch and protein films. Likewise, the fact that LF and its hydrolysate are large in size in comparison with the small antimicrobial compounds, such as nisin, pediocin, and lacticin, could lead to a slower diffusion from the polymer matrices to the film surface, limiting their antimicrobial effectiveness (Min and Khrocta, 2005).…”

“…The films with LAE (both CLAE and PLAE) had an absolute bactericidal effect at every control time. On the other hand, none of the formulations with LZ exhibited antilisterial activity, in spite of its notable enzyme activity (21.803 ± 2.378 U/mg), determined through the absorbance reduction caused by the lysis of Micrococcus lysodeikticus (Fabra et al, 2014). This could be attributed to the immobilization of LZ in the starch-gelatin matrix, which could limit its diffusion from the film surface, taking into account its high molecular weight (14.4 kDa).…”

“…Fabra et al (2014) pointed out the interaction of lysozyme when incorporated into starch films, which may affect the antimicrobial effectiveness of the films. Likewise, observed the influence of LAE concentration on its release kinetics from chitosan films; the higher the LAE concentration in the polymeric matrix, the slower the diffusion, due to an antiplasticizing effect.…”

“…This was expressed as units of lysozyme per mg (U/mg). One unit corresponds to a change in absorbance of 0.001 in 1min (Fabra et al, 2014). Each analysis was carried out in triplicate.…”

Fragrant starch-based films with limonene

“…fruit, where the film coating acts as a protective barrier for microorganisms and against drying. 3,4 A significant advantage of starch-based films is their biodegradability. They are tasteless and odorless.…”