Lysyl oxidase‐mediated VEGF‐induced differentiation and angiogenesis in human dental pulp cells

Bae, Won Kyung; Yi, Jin-Kyu; Park, J; Kang, Soo‐Kyung; Jang, Jun‐Hyeog; Kim, E C

doi:10.1111/iej.12796

Cited by 14 publications

(16 citation statements)

References 48 publications

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“…These effects were consistent with the BMP-2/CS/β-GP hydrogel delivery system [22]. On the one hand, the CS/β-GP delivery system saves cost and maximizes the effects of VEGF treatment [14,51,52]. On the other hand, the delivery system decreased the negative consequence caused by rapid loss of physical stability and bioactivity [22,32,[44][45][46].…”

Section: Stem Cells Internationalsupporting

confidence: 59%

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Zhou

et al. 2019

Stem Cells International

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The pulpotomy with pulp capping is aimed at retaining vital pulp with reparative dentin formation. Vascular endothelial growth factor (VEGF) plays a crucial role in dentin regeneration; however, its constant administrations in the human body is still problematic. Chitosan was widely studied as an effective carrier to deliver bioactive molecules in regenerative medicine. In this study, we conducted a chitosan/β-glycerophosphate (CS/β-GP) hydrogel as a VEGF-sustained release system and explored its effects on dental pulp stem cells (DPSCs). CS/β-GP hydrogel was manufactured using a sol-gel method. SEM assay showed the spongy and porous microstructure of the lyophilized hydrogels. DPSCs cultured in the CS/β-GP hydrogel kept adhesion and vitality. CCK-8 assay tested the promoted proliferation activity of DPSCs on the hydrogel. Besides, the added VEGF protein could continually release from VEGF/CS/β-GP hydrogel. The VEGF/CS/β-GP hydrogel could promote the odontogenic differentiation of DPSCs better than VEGF treatment without hydrogel. Our results suggested that CS/β-GP hydrogel could continually release VEGF and contribute to odontogenic differentiation of DPSCs, thus may become a potential carrier of bioactive molecules in pulp capping therapy.

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Section: Stem Cells Internationalsupporting

confidence: 59%

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Zhou

et al. 2019

Stem Cells International

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“…In contrast, LOX gene silencing and BAPN inhibition of LOX activity diminished the angiogenic effects of VEGF. Under these conditions, VEGF-induced phosphorylation of AKT, ERK, JNK and p38, and activation of NF-κB were inhibited by LOX silencing and BAPN [ 28 ]. Beta- aminopropionitrile (BAPN) inhibition of LOX activity, resulting in decreased phosphorylation of AKT and MAPK, was also noted during umbilical vein endothelial cell angiogenesis [ 29 ].…”

Section: Lox and Vascular Endothelial Growth Factormentioning

confidence: 99%

Lysyl Oxidase (LOX): Functional Contributions to Signaling Pathways

Laczkó

Csiszár

2020

Biomolecules

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Cu-dependent lysyl oxidase (LOX) plays a catalytic activity-related, primary role in the assembly of the extracellular matrix (ECM), a dynamic structural and regulatory framework which is essential for cell fate, differentiation and communication during development, tissue maintenance and repair. LOX, additionally, plays both activity-dependent and independent extracellular, intracellular and nuclear roles that fulfill significant functions in normal tissues, and contribute to vascular, cardiac, pulmonary, dermal, placenta, diaphragm, kidney and pelvic floor disorders. LOX activities have also been recognized in glioblastoma, diabetic neovascularization, osteogenic differentiation, bone matrix formation, ligament remodeling, polycystic ovary syndrome, fetal membrane rupture and tumor progression and metastasis. In an inflammatory context, LOX plays a role in diminishing pluripotent mesenchymal cell pools which are relevant to the pathology of diabetes, osteoporosis and rheumatoid arthritis. Most of these conditions involve mechanisms with complex cell and tissue type-specific interactions of LOX with signaling pathways, not only as a regulatory target, but also as an active player, including LOX-mediated alterations of cell surface receptor functions and mutual regulatory activities within signaling loops. In this review, we aim to provide insight into the diverse ways in which LOX participates in signaling events, and explore the mechanistic details and functional significance of the regulatory and cross-regulatory interactions of LOX with the EGFR, PDGF, VEGF, TGF-β, mechano-transduction, inflammatory and steroid signaling pathways.

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“…Leptin can induce angiogenesis, odontogenic differentiation, and mineralization in human DPCs [41]. VEGF promotes the in vitro angiogenesis of human DPCs through modulation of the expression of lysyl oxidase [46]. A specific distribution of NGFR was observed in the tooth buds and dental follicle [62].…”

Section: Discussionmentioning

confidence: 98%

Dental follicle stem cells rescue the regenerative capacity of inflamed rat dental pulp through a paracrine pathway

Hong

Chen

et al. 2020

Stem Cell Res Ther

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Background: Pulpitis is a common dental disease characterized by sustained inflammation and impaired pulp selfrepair. Mesenchymal stem cell-based minimally invasive vital pulp therapy (MSC-miVPT) is a potential treatment method, but its application is limited by the difficulty in acquiring MSCs. We recently revealed the immunomodulatory effects of rat dental follicle stem cells (rDFSCs) on acute lung injury. The present study focused on the paracrine effects of rDFSCs on the inflammation and regeneration of rat injured dental pulp to detect whether DFSCs are a potential candidate for MSC-miVPT. Methods: Conditioned medium from rDFSCs (rDFSC-CM) was applied to lipopolysaccharide (LPS)-induced inflammatory rat dental pulp cells (rDPCs). The inflammation and regeneration of rDPCs were detected by RT-qPCR, Western blotting, immunofluorescence staining, Cell Counting Kit-8 (CCK-8) assay, flow cytometry, wound-healing assay, and Masson's staining. The effects of rDFSC-CM on inflamed rat dental pulp were further evaluated by hematoxylin-eosin and immunohistochemical staining. Results: rDFSC-CM downregulated the ERK1/2 and NF-κB signaling pathways, which resulted in suppression of the expression of IL-1β, IL-6, and TNF-α and promotion of the expression of IL-4 and TGF-β, and these findings lead to the attenuation of rDPC inflammation. rDFSC-CM enhanced the in vitro proliferation, migration, and odontogenic differentiation of inflammatory rDPCs and their in vivo ectopic dentinogenesis. Furthermore, rDFSC-CM inhibited inflammatory cell infiltration in rat pulpitis and triggered Runx2 expression in some of the odontoblast-like cells surrounding the injured site, and these effects were conducive to the repair of inflamed dental pulp. Conclusions: rDFSC-CM exhibits therapeutic potential by rescuing the regeneration of the inflamed rat dental pulp through an immunomodulatory mechanism, indicating the application prospects of DFSCs in biological regenerative endodontics.

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Lysyl oxidase‐mediated VEGF‐induced differentiation and angiogenesis in human dental pulp cells

Abstract: Recombinant human vascular endothelial growth factor promoted cell growth, odontogenic potential and in vitro angiogenesis via modulation of LOX expression. These results support the concept that rhVEGF may offer therapeutic benefits in regenerative endodontics.

Cited by 14 publications

References 48 publications

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Lysyl Oxidase (LOX): Functional Contributions to Signaling Pathways

Dental follicle stem cells rescue the regenerative capacity of inflamed rat dental pulp through a paracrine pathway

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