Lytic regulated cell death in aquaculture fish

Xia, Xiaojing; He, Binglin; Zhang, Xiulin; Cheng, Zhe; Liu, Mingcheng; Wei, Xiaobing; Jiang, Jinqing; Hu, Jianhe

doi:10.1111/raq.12533

Cited by 5 publications

(2 citation statements)

References 166 publications

(341 reference statements)

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“…In this study, genes were upregulated related to necroptosis pathway, namely, TNFSF10, caspase 1, cytochrome B, CAMK2B, and SLC25A4 ( Table 2 ). In fish, necroptosis is also responsible for stimulating physiological functions such as the immune defence, inflammatory reactions and plays an important role in the process of maintaining cellular homeostasis ( Xia et al, 2021 ). Hence, upregulation of pathways might be related to immune response of the L. rohita to salinity stress.…”

Section: Discussionmentioning

confidence: 99%

Integrative miRNA-mRNA network analysis to identify crucial pathways of salinity adaptation in brain transcriptome of Labeo rohita

Shukla,

Vemula,

Raval

et al. 2023

Front. Genet.

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Introduction: Brain being the master regulator of the physiology of animal, the current study focuses on the gene expression pattern of the brain tissue with special emphasis on regulation of growth, developmental process of an organism and cellular adaptation of Labeo rohita against unfavourable environmental conditions.Methods: RNA-seq study was performed on collected brain samples at 8ppt salt concentration and analyzed for differential gene expression, functional annotation and miRNA-mRNA regulatory network.Results: We found that 2450 genes were having significant differential up and down regulation. The study identified 20 hub genes based on maximal clique centrality algorithm. These hub genes were mainly involved in various signaling pathways, energy metabolism and ion transportation. Further, 326 up and 1214 down regulated genes were found to be targeted by 7 differentially expressed miRNAs i.e., oni-miR-10712, oni-miR-10736, ssa-miR-221-3p, ssa-miR-130d-1-5p, ssa-miR-144-5p and oni-miR-10628. Gene ontology analysis of these differentially expressed genes led to the finding that these genes were involved in signal transduction i.e., calcium, FOXO, PI3K-AKT, TGF-β, Wnt and p53 signalling pathways. Differentially expressed genes were also involved in regulation of immune response, environmental adaptation i.e., neuroactive ligand-receptor interaction, ECM-receptor interaction, cell adhesion molecules and circadian entrainment, osmoregulation and energy metabolism, which are critical for salinity adaptation.Discussion: The findings of whole transcriptomic study on brain deciphered the miRNA-mRNA interaction patterns and pathways associated with salinity adaptation of L. rohita.

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Section: Discussionmentioning

confidence: 99%

Integrative miRNA-mRNA network analysis to identify crucial pathways of salinity adaptation in brain transcriptome of Labeo rohita

Shukla,

Vemula,

Raval

et al. 2023

Front. Genet.

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“…Among other heavy metals, Pb especially encourages iron-initiated membrane lipid oxidation in fish [21], which is considered an essential mediator of ferroptosis, a lytic form of regulated cell death (LRCD) [71]. Although nonmammalian vertebrates, including fish, exhibit such an oxidation pathway, the extent to which ferroptosis per se is involved is still poorly understood [72].…”

Section: Morphological Modificationsmentioning

confidence: 99%

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Macirella

Curcio

Ahmed

et al. 2023

Fishes

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Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.

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Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Martín-Manzo,

Morelos-Castro,

Munguia-Vega

et al. 2024

Mol Biol Rep

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The river prawn, Macrobrachium americanum (M. americanum), is one of the largest prawns of the genus in Latin America and is an amphidromous species distributed along the Paci c coast of America. This prawn has commercial value due to its size and taste, making it a good option for aquaculture production. Its culture has been attempted in ponds and concrete tanks, but no successful technique can still support commercial production. Understanding the mechanisms that regulate reproduction at the molecular level is very important. This knowledge can provide tools for manipulating transcripts, which could increase the number or size of animals in the culture. However, our understanding of the mechanism that regulates the reproduction of M. americanum at the molecular level is limited. In this study, we performed transcriptome assembly of the testes, vas deferens, and terminal ampulla of M. americanum. The cDNA library was constructed and sequenced for each tissue to identify novel transcripts. A combined transcriptome with the three tissues was assembled using Trinity software.Unigenes were annotated using BLASTx and BLAST2GO. The transcriptome assembly generated 1,059,447 unigenes, of which 7,222 genes had signi cant hits (e-value < 1x10 − 5 ) when compared against the Swiss-Prot database. Around 75 genes were related to sex determination, testis development, spermatogenesis, spermiogenesis, fertilization, maturation of testicular cells, neuropeptides, hormones, hormone receptors, and/or embryogenesis. These results provide new molecular information about M. americanum reproduction, representing a reference point for further genetic studies of this species.

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Lytic regulated cell death in aquaculture fish

Cited by 5 publications

References 166 publications

Integrative miRNA-mRNA network analysis to identify crucial pathways of salinity adaptation in brain transcriptome of Labeo rohita

Integrative miRNA-mRNA network analysis to identify crucial pathways of salinity adaptation in brain transcriptome of Labeo rohita

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

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