LZAP promotes the proliferation and invasiveness of cervical carcinoma cells by targeting AKT and EMT

Dai, Yifang; Lin, Ning; He, Deqin; Xu, Mu; Zhong, Li-Ying; He, Shuqiong; Guo, Danhua; Li, Ying; Huang, Hailong; Zheng, Xuemei; Xu, Liangpu

doi:10.7150/jca.39359

Cited by 7 publications

(11 citation statements)

References 26 publications

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“…Accumulating evidence indicates that CDK5RAP3 has been implicated in governing cellular signal transduction including the p53 ( 2 , 15 ), NF-κB ( 5 , 8 , 9 , 48 ), Wnt ( 20 , 24 , 25 ), wild-type p53-induced phosphatase 1 (Wip 1) ( 10 , 33 ), AKT ( 27 , 30 ) and signal transducer and activator of transcription 3 (STAT3) signaling cascades ( 17 ). As previously described, CDK5RAP3 positively regulates p53 activity either in the presence or absence of ARF or independently of p53 mutation state ( 2 , 15 ).…”

Section: Biological Functions Of Cdk5rap3mentioning

confidence: 99%

“…The study from Zheng et al., also showed that CDK5RAP3 suppresses the inhibitory phosphorylation of GSK3β via inhibiting AKT phosphorylation in gastric cancer ( 25 ). Moreover, their group demonstrated that CDK5RAP3 modulates AKT signaling in both gastric neuroendocrine and cervical carcinoma ( 27 , 30 ). CDK5RAP3 also negatively regulates AKT/hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor A (VEGFA) pathway in gastric neuroendocrine carcinoma (GNEC) ( Figure 5D ).…”

Section: Biological Functions Of Cdk5rap3mentioning

confidence: 99%

“…CDK5RAP3 is also observed to enhance the clonogenesis and migration in breast cancer cells ( 17 ). Fourth, CDK5RAP3 is markedly overexpressed in cervical carcinoma based on the data from several online databases and enhances the proliferation and tumorigenicity in cervical carcinoma cells ( 30 ). In addition, several CDK5RAP3 isoforms are observed to show an oncogenic phenotype ( 84 , 85 ).…”

Section: Cdk5rap3 and Diseasementioning

confidence: 99%

“…Hereafter, it displays a tumor suppressor activity in hepatocellular carcinoma ( 23 ), gastric cancer ( 16 , 24 – 27 ), and renal cancer ( 28 ) as well. In contrast, additional studies indicate that CDK5RAP3 appears to promote tumorigenesis because it is overexpressed in lung adenocarcinoma ( 29 ), hepatocellular carcinoma ( 11 , 14 ), breast cancer ( 17 ) and cervical carcinoma ( 30 ), and has an oncogenic roles in these cancers. Hence, the roles of CDK5RAP3 in human cancers remain elusive and controversial.…”

Section: Introductionmentioning

confidence: 99%

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Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 3: Potential Functions and Implications for Development and Disease

Sheng

Rao

et al. 2021

Front. Oncol.

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Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. To date, CDK5RAP3 has been reported to interact with a range of proteins involved in cellular events ranging from cell cycle, apoptosis, and invasion to UFMylation modification and endoplasmic reticulum stress. Owing to its crucial roles in cellular processes, CDK5RAP3 is demonstrated to be not only an active participant in embryonic and mammalian tissue development, but also a key regulator in the onset and progress of human cancers such as head and neck squamous cell carcinoma, gastric cancer, hepatocellular cancer, lung cancer, kidney cancer and breast cancer. Notwithstanding, the detailed function of CDK5RAP3 and its mechanism remain poorly defined. Here, we briefly described a history of the discovery of CDK5RAP3, and systematically overviewed its gene structural and distribution features. We also focused on the known functions of this protein and its implications for embryogenesis and tissue development, as well as diseases especially carcinoma. This review may facilitate to understand the molecular and functional basis of CDK5RAP3 and its association with development and disease, and provide a reasonable idea for novel therapeutic opportunities targeting CDK5RAP3.

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Section: Biological Functions Of Cdk5rap3mentioning

confidence: 99%

Section: Biological Functions Of Cdk5rap3mentioning

confidence: 99%

Section: Cdk5rap3 and Diseasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 3: Potential Functions and Implications for Development and Disease

Sheng

Rao

et al. 2021

Front. Oncol.

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“…Cervical squamous cell carcinoma (CSCC) is a common cancer; it is the fourth most frequently diagnosed cancer and fourth leading cause of cancer-related death in women ( 1 ). Although many novel treatment methods and technologies have been used in CSCC, the clinical outcomes of patients have not been satisfactory ( 2 ). Some studies have reported that the 5-year overall survival of CSCC patients is still less than 17% ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel circRNA, hsa_circ_0065898, that regulates tumor growth in cervical squamous cell carcinoma

Ni¹,

Liu²,

Deng³

2021

Transl Cancer Res TCR

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Background: Circular RNAs (circRNAs) were reported to play an important role in regulating tumor pathogenesis. The molecular mechanism of circRNAs in cervical squamous cell carcinoma (CSCC) remains poorly understood. We aimed to identify the circRNAs differentially expressed, and to investigate the role of a novel circRNA, hsa_circ_0065898, in regulating proliferation, migration, and invasion in CSCC.Methods: The online Kaplan-Meier Plotter was used to analyze the relationship between miRNA expression and overall survival. Bioinformatics tools, such as R, Cytoscape, and Perl, were used to analyze the Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, proteinprotein interaction (PPI) network, and regulatory network. The expression level of hsa_circ_0065898 in CSCC cell lines was evaluated using quantitative polymerase chain reaction in vitro. The cell counting kit-8 (CCK-8) and transwell assays were used to assess cell proliferation, migration, and invasion.Results: circRNA expression data (GSE102686) was downloaded from the Gene Expression Omnibus database, and this included data from 5 CSCC patients and 5 normal tissues. 13 differentially expressed circRNAs were identified, which included 9 upregulated circRNAs and 4 downregulated circRNAs. GO enrichment analysis showed that the target genes of miRNAs associated with hsa_circ_0065898 were enriched in ubiquitin-protein transferase activity, ubiquitin-like protein transferase activity, core promoter sequence-specific DNA binding, mRNA 3'-UTR AU-rich region binding, core promoter binding, and so on. KEGG showed that the Hippo and p53 signaling pathways played significant role in the pathway network.Hsa_circ_0065898 was significantly overexpressed in the CSCC cell lines. Hsa_circ_0065898 facilitated cell proliferation, migration, and invasion in CSCC.Conclusions: This study identified differentially expressed circRNAs and constructed the regulatory network of hsa_circ_0065898 targeting microRNAs and mRNAs. We demonstrated that hsa_circ_0065898 promoted CSCC cell proliferation, migration, and invasion. Hence, hsa_circ_0065898 might be useful as a biomarker for CSCC diagnosis and targeted therapy.

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The emerging roles of UFMylation in the modulation of immune responses

Liang,

Ning,

Wang

et al. 2024

Clinical & Translational Med

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Post‐translational modification is a rite of passage for cellular functional proteins and ultimately regulate almost all aspects of life. Ubiquitin‐fold modifier 1 (UFM1) system represents a newly identified ubiquitin‐like modification system with indispensable biological functions, and the underlying biological mechanisms remain largely undiscovered. The field has recently experienced a rapid growth of research revealing that UFMylation directly or indirectly regulates multiple immune processes. Here, we summarised important advances that how UFMylation system responds to intrinsic and extrinsic stresses under certain physiological or pathological conditions and safeguards immune homeostasis, providing novel perspectives into the regulatory framework and functions of UFMylation system, and its therapeutic applications in human diseases.

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LZAP promotes the proliferation and invasiveness of cervical carcinoma cells by targeting AKT and EMT

Cited by 7 publications

References 26 publications

Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 3: Potential Functions and Implications for Development and Disease

Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 3: Potential Functions and Implications for Development and Disease

Identification of a novel circRNA, hsa_circ_0065898, that regulates tumor growth in cervical squamous cell carcinoma

The emerging roles of UFMylation in the modulation of immune responses

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