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Elks, J.; Ganellin, C. Robin

doi:10.1007/978-1-4757-2085-3_13

Cited by 2 publications

(2 citation statements)

References 1,093 publications

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“…Transcriptional regulation of members of the PDE3 and PDE4 families has been described. PDE3B expression is increased upon dierentiation of 3T3-L1 ®broblasts to 3T3-L1 adipocytes (Elks et al, 1983). Transcriptional regulation of PDE4 activity has been studied in Sertoli cells and most recently in monocytes (Swinnen et al, 1989;Torphy et al, 1992;Verghese et al, 1995;Manning et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Cyclic AMP‐mediated regulation of vascular smooth muscle cell cyclic AMP phosphodiesterase activity

Rose

Liu

Palmer

et al. 1997

British J Pharmacology

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1 Rat cultured aortic vascular smooth muscle cells (VSMC) express both cyclic GMP-inhibited cyclic AMP phosphodiesterase (PDE3) and Ro 20-1724-inhibited cyclic AMP phosphodiesterase (PDE4) activities. By utilizing either cilostamide, a PDE3-selective inhibitor, or Ro 20-1724, a PDE4-selective inhibitor, PDE3 and PDE4 activities were shown to account for 15% and 55% of total VSMC cyclic AMP phosphodiesterase (PDE) activity. 2 Treatment of VSMC with either forskolin or 8-bromo-cyclic AMP caused signi®cant concentrationand time-dependent increases in total cellular cyclic AMP PDE activity. Using cilostamide or Ro 20-1724, we demonstrated that both PDE3 and PDE4 activities were increased following forskolin or 8-bromo-cyclic AMP treatment, with a relatively larger e ect observed on PDE3 activity. The increase in cyclic AMP PDE activity induced by forskolin or 8-bromo-cyclic AMP was inhibited by actinomycin D or cycloheximide, demonstrating that new mRNA synthesis and protein synthesis were required. An analogue of forskolin which does not activate adenylyl cyclase (1,9-dideoxyforskolin) or an analogue of cyclic GMP (8-bromo-cyclic GMP) did not a ect total cyclic AMP PDE activity. 3 Incubation of VSMC with 8-bromo-cyclic AMP for 16 h caused a marked rightward shift in the concentration-response curves for both isoprenaline-and forskolin-mediated activation of adenylyl cyclase. A role for up-regulated cyclic AMP PDE activity in this reduced potency is supported by our observation that cyclic AMP PDE inhibitors (IBMX, cilostamide or Ro 20-1724) partially normalized the e ects of isoprenaline or forskolin in treated cells to those in untreated cells. 4 We conclude that VSMC cyclic AMP PDE activity is increased following long-term elevation of cyclic AMP and that increases in PDE3 and PDE4 activities account for more than 70% of this e ect. Furthermore, we conclude that increases in cyclic AMP PDE activity contribute to the reduced potency of isoprenaline or forskolin in treated VSMC. These results have implications for long-term use of cyclic AMP PDE inhibitors as therapeutic agents.

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Section: Introductionmentioning

confidence: 99%

Cyclic AMP‐mediated regulation of vascular smooth muscle cell cyclic AMP phosphodiesterase activity

Rose

Liu

Palmer

et al. 1997

British J Pharmacology

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“…In intact cells, type III (or cGI) PDEs are activated by insulin and catecholamines or other agents that increase cellular cAMP concentration [11]. The regulation of cGI PDE in rat white adipose cells and 3T3-L1 adipocytes has been well characterized [12][13][14][15][16][17][18]. In white adipose cells, insulin decreases intracellular cAMP concentration and exerts its anti-lipolytic action, at least in part, via activation of cGI PDE.…”

Section: Introductionmentioning

confidence: 99%

Insulin stimulates hormone‐sensitive cyclic GMP‐inhibited cyclic nucleotide phosphodiesterase in rat brown adipose cells

Omatsu‐Kanbe¹,

Cushman²,

Manganiello³

et al. 1995

FEBS Letters

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The presence and regulation of a hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase (cGl PDE) in rat brown adipose cells was investigated, cDNA clones for two cGl PDE isoforms, cGIPI and cGIP2, have been isolated. Using a rat cGIPI (RcGIPI) cDNA probe, RcGIPI mRN A (~5.3 kb) was detected in Northern blots of both brown and white adipose RNA. cGl PDE was detected in both microsomal and plasma membrane fractions of brown and white adipose cells by Western blotting using anti-RcGIPl peptide antibody. When cells were incubated with insulin before membrane preparation, cGl PDE activity in the microsomal fraction was increased by 2-to 2.5-fold within I0 min. Isoproterenol also stimulated the activity of cGl PDE in the microsomal fraction by 1.5-fold. In cells incubated with both insulin and isoproterenol, microsomal cGl PDE activity was similar to that in microsomal fractions isolated from cells incubated with insulin alone. These results suggest that the hormonal regulation of cGl PDE, presumably a cGIPI isoform, in rat brown adipose cells is similar to that in white adipose cells.

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M

Cited by 2 publications

References 1,093 publications

Cyclic AMP‐mediated regulation of vascular smooth muscle cell cyclic AMP phosphodiesterase activity

Cyclic AMP‐mediated regulation of vascular smooth muscle cell cyclic AMP phosphodiesterase activity

Insulin stimulates hormone‐sensitive cyclic GMP‐inhibited cyclic nucleotide phosphodiesterase in rat brown adipose cells

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