M &amp; B 693 for Gonorrhoea

Gardner, Aleeza N.

doi:10.1136/bmj.1.4085.845

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, the small amount of RNA (Table I) seems to rule out significant contamination by rough-surfaced microsomes in the Golgi fraction . 4 It has been postulated that certain glycosyl transferases are localized in the Golgi apparatus and thus could serve as marker enzymes for this 4 The presence of RNA in different types of "smoothsurfaced membranes" is a matter of discussion, since theoretically it can be due either to ribosomes, subunits of ribosomes or to "membrane-constituents" (47)(48)(49) .…”

Section: Subcellular Fractionsmentioning

confidence: 99%

Evidence for the Participation of the Golgi Apparatus in the Intracellular Transport of Nascent Albumin in the Liver Cell

Glaumann

Ericsson

1970

The Journal of Cell Biology

117

View full text Add to dashboard Cite

A comparative biochemical and radioautographic in vivo study was performed to identify the site of synthesis and route of migration of albumin in the parenchymal liver cell after labeling with leucine-' 4 C or leucine-3 H via the portal vein. Free cytoplasmic ribosomes, membrane-bound ribosomes, rough-and smooth-surfaced microsomes, and Golgi membranes were isolated . The purity of the Golgi fraction was examined morphologically and biochemically. After administration of leucine-' 4C, labeled albumin was extracted, and the sequence of transport was followed from one fraction to the other . Approximately 2 min after the intravenous injection, bound ribosomes displayed a maximal rate of leucine-14C incorporation into albumin . 4 min later, a peak was reached for rough microsomes.Corresponding maximal activities for smooth microsomes were recorded at 15 min, and for the Golgi apparatus at -20 min . The relative amount of albumin, calculated on a membrane protein basis, was higher in the Golgi fraction than in the microsomes . By radioautography the silver grains were preferentially localized over the rough-surfaced endoplasmic reticulum at the 5 min interval . Apparent activity in the Golgi zone was noted 9 min after the injection ; at 15 and 20 min, the majority of the grains were found in this location. Many of the grains associated with the Golgi apparatus were located over Golgi vacuoles containing 300-800 A electron-opaque bodies . It is concluded that albumin is synthesized on bound ribosomes, subsequently is transferred to the cavities of rough-surfaced endoplasmic reticulum, and then undergoes migration to the smooth-surfaced endoplasmic reticulum and the Golgi apparatus . In the latter organelle, albumin can be expected to be segregated together with very low density lipoprotein in vacuoles known to move toward the sinusoidal portion of the cell and release their content to the blood .

show abstract

Section: Subcellular Fractionsmentioning

confidence: 99%

Evidence for the Participation of the Golgi Apparatus in the Intracellular Transport of Nascent Albumin in the Liver Cell

Glaumann

Ericsson

1970

The Journal of Cell Biology

117

View full text Add to dashboard Cite

show abstract

“…Other halogen replacement reactions are demonstrated by substitutions by trialkylsilyloxy (364), phosphine (74,117), and methyl (11) groups. Dimethylboron chloride and methylboron dichloride were said to be unstable, When di-n-butylboron chloride was reacted with sodium or potassium in ether and the solvent evaporated, tri-n-butylboron and a solid product of the empirical formula BN were obtained (11).…”

Section: Chemical Propertiesmentioning

confidence: 99%

Organic Compounds Of Boron

Läppert¹

1956

Chem. Rev.

205

View full text Add to dashboard Cite

“…Immunization of rhesus macaques at the California Primate Research Center with a Formalin-inactivated SRV-1 vaccine elicited neutralizing antibodies and protected the animals from experimental infection by . Subsequent immunization with denatured, nonglycosylated SRV-1 envelope antigens expressed in yeast cells did not elicit neutralizing antibody (17) or protect against experimental infection with this virus (9). At the Washington Primate Research Center, pigtailed macaques (M. nemestrina) immunized with a recombinant vaccinia virus expressing the envelope glycoproteins of SRV-2 were protected against challenge infection with this virus (15).…”

mentioning

confidence: 99%

Protein of macaques against infection with simian type D retrovirus (SRV-1) by immunization with recombinant vaccinia virus expressing the envelope glycoproteins of either SRV-1 or Mason-Pfizer monkey virus (SRV-3)

Brody

Hunter

Kluge

et al. 1992

J Virol

View full text Add to dashboard Cite

Rhesus macaques were immunized with live vaccinia virus recombinants expressing the envelope glycoproteins (gp7O and gp22) of simian type D retrovirus (SRV), serotype 1 or 3. All of the animals immunized with either the SRV-1 env or the SRV-3 env vaccinia virus recombinant developed neutralizing antibodies against the homologous SRV. In addition, both groups developed cross-reactive antibodies and were protected against an intravenous live-virus challenge with SRV-1. The four control animals immunized with a vaccinia virus recombinant expressing the G protein of respiratory syncytial virus were not protected against the same SRV-1 challenge. Although SRV-1 and SRV-3 immune sera showed cross-neutralization, they failed to neutralize a separate, more distantly related serotype, SRV-2, in an in vitro assay. These findings are consistent with the known degree of serologic and genetic relatedness of these three SRV strains.

show abstract

M & B 693 for Gonorrhoea

Cited by 3 publications

References 0 publications

Evidence for the Participation of the Golgi Apparatus in the Intracellular Transport of Nascent Albumin in the Liver Cell

Evidence for the Participation of the Golgi Apparatus in the Intracellular Transport of Nascent Albumin in the Liver Cell

Organic Compounds Of Boron

Protein of macaques against infection with simian type D retrovirus (SRV-1) by immunization with recombinant vaccinia virus expressing the envelope glycoproteins of either SRV-1 or Mason-Pfizer monkey virus (SRV-3)

Contact Info

Product

Resources

About