M gene analysis of canine coronavirus strains detected in Korea

Abstract

The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid sequence) and 87.7% to 97.8% (92.4% to 100% for the amino acid sequence) when compared to FCoV-like CCV strains (type I). Three amino acid variations in the M gene were characteristic for the Korean CCV strains. Phylogenetic analysis demonstrated that the 22 Korean CCV strains belonged to four typical CCV clusters (i.e., a unique Korean CCV cluster, a type II and transmissible gastroenteritis virus cluster, an intermediate cluster between type I and II, and a type I cluster). This study was the first to identify genetic differences of the M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.

Co-Circulation of Canine Coronavirus I and IIa/b with High Prevalence and Genetic Diversity in Heilongjiang Province, Northeast China

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Evolutionary genetics of canine respiratory coronavirus and recent introduction into Swedish dogs

“…Additional surveys have identified antibodies in dogs in the UK, Ireland, Italy, Japan, and the US, with antibody prevalence as high as 87.7% in the state of Kentucky, USA (An et al, 2010b;Decaro et al, 2007;Erles and Brownlie, 2005;Knesl et al, 2009;Priestnall et al, 2006;Priestnall et al, 2007;Schulz et al, 2014). A small number of isolates have also been sequenced and characterized (An et al, 2010a;Erles et al, 2007;Erles et al, 2003;Jeoung et al, 2014;Yachi and Mochizuki, J o u r n a l P r e -p r o o f 4 closely related species or strains such as Sambaar deer coronavirus, Waterbuck coronavirus and human enteric coronavirus (HEC 4408), illustrating the proliferation of these BCoV-like viruses.…”

Molecular characterization of canine parvovirus and canine enteric coronavirus in diarrheic dogs on the island of St. Kitts: First report from the Caribbean region

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