M13-Tailed Primers Improve the Readability and Usability of Microsatellite Analyses Performed with Two Different Allele-Sizing Methods

Boutin-Ganache, Isabelle; Raposo, Marco; Raymond, Martine; Deschepper, Christian F.

doi:10.2144/01311bm02

Cited by 571 publications

(395 citation statements)

References 2 publications

Supporting

Mentioning

392

Contrasting

Unclassified

Order By: Relevance

“…FH2202 is located near the DLA-DRB2 pseudogene in the domestic dog (21) and is located in the 216.8-kb region spanned by CFA12-4 and CFA12-13 (S.D., unpublished data). The forward primers were augmented on the 5Ј end with the addition of an M13 sequence, and a dye-labeled M13 primer was included in the PCR reaction (22). The hybrid forward-M13 primer and the labeled M13 primer were used in limiting concentration in the PCR (2.5 m each).…”

Section: Methodsmentioning

confidence: 99%

High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal

Aguilar

Roemer

Debenham

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

374

353

View full text Add to dashboard Cite

The San Nicolas Island fox (Urocyon littoralis dickeyi) is genetically the most monomorphic sexually reproducing animal population yet reported and has no variation in hypervariable genetic markers. Such low levels of variation imply lower resistance to pathogens, reduced fitness, and problems in distinguishing kin from non-kin. In vertebrates, the MHC contains genes that influence disease resistance and kin recognition and may be under intense balancing selection in some populations. Hence, genetic variation at the MHC might persist despite the extreme monomorphism shown by neutral markers. We examine variation of five loci within the MHC of San Nicolas Island foxes and find remarkably high levels of variation. Further, we show by simulation that genetic monomorphism at neutral loci and high MHC variation could arise only through an extreme population bottleneck of <10 individuals, Ϸ10 -20 generations ago, accompanied by unprecedented selection coefficients of >0.5 on MHC loci. These results support the importance of balancing selection as a mechanism to maintain variation in natural populations and expose the difficulty of using neutral markers as surrogates for variation in fitness-related loci.T he island fox (Urocyon littoralis) is an endemic North American canid that inhabits six of the eight Channel Islands off the coast of southern California (Fig. 1). As suggested by the archeological record and molecular genetic data, foxes colonized the three northern Channel Islands (San Miguel, Santa Rosa, and Santa Cruz) Ϸ16,000 years ago and, subsequently, were transported by Native Americans to the three southern Channel Islands (San Nicolas, Santa Catalina, and San Clemente) 800 to 4,300 years ago (1-4) ( Fig. 1). Effective population size varies with island area and ranges from Ͻ200 to Ϸ1,000 individuals (Table 1). Levels of genetic variation reflect population size and colonization history, with the San Nicolas Island population having the second smallest effective population size and a recent colonization history (4) ( Fig. 1 and Table 1). No variation has been discovered for any of four independent genetic marker classes in the San Nicolas Island population, including supposedly neutral hypervariable microsatellite loci (5) and multilocus fingerprints (2), for which the probability of genetic identity is commonly Ͻ1 in several million (6). Recently, because of dramatic declines, populations on the three Northern Islands and Santa Catalina Island have been proposed for listing under the U.S. Endangered Species Act (7).The MHC contains the most variable set of coding genes in vertebrates, with as many as 349 alleles described for a single locus (8) and heterozygosity values that generally exceed those predicted by neutrality (9). Class I and II MHC molecules are responsible for the presentation to T cells of intracellular (endogenous) and extracellular (exogenous) peptides, respectively (10). High levels of heterozygosity at the MHC may be maintained by balancing selection through pathogen-mediated selection, ...

show abstract

Section: Methodsmentioning

confidence: 99%

High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal

Aguilar

Roemer

Debenham

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

374

353

View full text Add to dashboard Cite

show abstract

“…These primers were selected based on SSR loci isolated by the French Centre de Coopération en Recherche Agronomique pour le Dévelopment (CIRAD) and Malaysian Palm Oil Board (MPOB) ( Table 1). The forward primers were added with M13 sequence 5'-GGA AAC AGC TAT GAC CAT-3' (Oetting et al, 1995), permitting concurrent fluorescence labelling of polymerate chain reaction (PCR) products by a third primer (M13) with an incorporated multiple fluorescent dye label (6-FAM™, VIC ® , NED™ and PET ® ) together with the reverse primer (Boutin-Ganache et al, 2001).…”

Section: Microsatellite Analysismentioning

confidence: 99%

Legitimacy Assessment of Pollen With Simple Sequence Repeats Markers

Kwan¹

2017

JOPR

View full text Add to dashboard Cite

show abstract

“…13 The mapped interval was then compared with the published sequence of rat chromosome 5 (build 3.1, available online at http://www.ncbi.nlm.nih.gov/mapview).…”

Section: Genetic Mappingmentioning

confidence: 99%

A Genetic Locus Accentuates the Effect of Volume Overload on Adverse Left Ventricular Remodeling in Male and Female Rats

et al. 2006

Self Cite

View full text Add to dashboard Cite

Abstract-Although increased left ventricular (LV) mass is highly predictive of cardiovascular morbidity and mortality in humans, it has never been verified in an experimental model that naturally occurring alleles linked to increased LV mass under basal conditions also associate with worsened cardiovascular prognosis. Because we have shown previously that locus Cm24 on chromosome 5 was responsible for differences in LV mass between WKY and WKHA rats, we used WKY.WKHA-(D5Rat45-D5Rat245) congenic rats (where locus Cm24 has been transferred from WKHA into WKY rats) to test how naturally occurring gene variants present in Cm24 would, in addition to their effects under basal conditions, affect LV mass remodeling and/or function in the context of overload. Volume overload was induced in WKY, WKHA, and WKY.WKHA congenic rats by surgical creation of an aorto-caval fistula. In females, the fistula had no effect on the hearts of WKY rats, yet it induced dilated eccentric hypertrophy and isolated diastolic dysfunction in WKHA and WKY.WKHA congenic rats, along with signs of congestive heart failure. In males, the surgical maneuver induced only mild or inconsistent responses in WKY rats but had much more pronounced effects in WKHA and WKY.WKHA congenic rats. Altogether, our data show that a genetic locus that induces, under basal conditions, either mild or no concentric LV remodeling in either male or female rats, respectively, associates with LV dilatation and dysfunction in both sexes when the hearts are additionally challenged. Key Words: hypertrophy Ⅲ myocardium Ⅲ myocytes Ⅲ heart failure Ⅲ genetics Ⅲ gender Ⅲ natriuretic peptides C oncentric left ventricular (LV) hypertrophy (LVH) is a frequent complication of hypertension. In adult humans, concentric LVH is highly predictive of cardiovascular morbidity and mortality, 1,2 the relationship being so strong that cardiovascular risk increases continuously and proportionally with LV mass (LVM). 3 Although demographic and anthropometric variables (including blood pressure) affect 25% to 50% of the variance of LVM, the remaining 50% to 75% of the variance is accounted for by other (presumably genetic) factors. 2 With the goal of finding genetic factors linked to the regulation of LVM, we have previously studied crosses of WKY and WKHA inbred rats, where WKHA rats display LVH in comparison to WKY rats despite identical and normal blood pressures. 4 These efforts have allowed us to detect on rat chromosome 5 a quantitative trait locus (QTL) that was linked to LVM in the male (but not the female) progeny under basal conditions. 5 This QTL is now identified by the symbol Cm24 in the Rat Genome Database (available online at http://rgd.mcw.edu/). In a follow-up study, we generated congenic strains where a small segment of chromosome 5 from WKHA rats (containing QTL Cm24) was introgressed into the genetic background of the WKY strain. 6 In one of these strains [designated as WKY.WKHA-(D5Rat45-D5Rat245)], cardiomyocytes (CMs) isolated from adult male hearts were wider than CMs isolate...

show abstract

M13-Tailed Primers Improve the Readability and Usability of Microsatellite Analyses Performed with Two Different Allele-Sizing Methods

Cited by 571 publications

References 2 publications

High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal

High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal

Legitimacy Assessment of Pollen With Simple Sequence Repeats Markers

A Genetic Locus Accentuates the Effect of Volume Overload on Adverse Left Ventricular Remodeling in Male and Female Rats

Contact Info

Product

Resources

About