M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

Zhou, Yikun; Han, Chun-shan; Zhu, Zilu; Chen, Peng; Wang, Yiming; Lin, Shuai; Chen, Liu-jing; Zhuang, Zi-meng; Yang, Ruili

doi:10.1016/j.bioactmat.2023.08.006

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…It is inconsistent with our present study which proved that the exosomes derived from M2 macrophages promoted the proliferation and osteogenic differentiation of BMMSCs. More recently, a study by Zhou et al indicated that exosomes from M2 macrophages promote the osteogenic differentiation of BMMSCs, which is consistent with our results [ 38 ]. This discrepancy can be attributed to the differences in cell lineage of macrophages and culture conditions.…”

Section: Discussionsupporting

confidence: 93%

Exosomes derived from M2 macrophages prevent steroid-induced osteonecrosis of the femoral head by modulating inflammation, promoting bone formation and inhibiting bone resorption

Yuan,

Zhang,

Yang

et al. 2024

J Orthop Surg Res

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Inflammatory reactions are involved in the development of steroid-induced osteonecrosis of the femoral head(ONFH). Studies have explored the therapeutic efficacy of inhibiting inflammatory reactions in steroid-induced ONFH and revealed that inhibiting inflammation may be a new strategy for preventing the development of steroid-induced ONFH. Exosomes derived from M2 macrophages(M2-Exos) display anti-inflammatory properties. This study aimed to examine the preventive effect of M2-Exos on early-stage steroid-induced ONFH and explore the underlying mechanisms involved. In vitro, we explored the effect of M2-Exos on the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells(BMMSCs). In vivo, we investigated the role of M2-Exos on inflammation, osteoclastogenesis, osteogenesis and angiogenesis in an early-stage rat model of steroid-induced ONFH. We found that M2-Exos promoted the proliferation and osteogenic differentiation of BMMSCs. Additionally, M2-Exos effectively attenuated the osteonecrotic changes, inhibited the expression of proinflammatory mediators, promoted osteogenesis and angiogenesis, reduced osteoclastogenesis, and regulated the polarization of M1/M2 macrophages in steroid-induced ONFH. Taken together, our data suggest that M2-Exos are effective at preventing steroid-induced ONFH. These findings may be helpful for providing a potential strategy to prevent the development of steroid-induced ONFH.

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Section: Discussionsupporting

confidence: 93%

Exosomes derived from M2 macrophages prevent steroid-induced osteonecrosis of the femoral head by modulating inflammation, promoting bone formation and inhibiting bone resorption

Yuan,

Zhang,

Yang

et al. 2024

J Orthop Surg Res

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“…Exosomes were vesicles with a diameter of 30–200 nm released by cells into the extracellular space and an important application in tissue engineering was as a nanocarrier for loading proteins, nucleic acids and drugs. Exosomes entered target cells mainly through membrane fusion, and the signaling molecules they carried acted on a variety of signaling pathways in target cells to participate in intercellular information transfer [ 32 , 33 ]. Therefore, the internalization of exosomes by target cells was the basis and premised for their biological function regulation of target cells.…”

Section: Discussionmentioning

confidence: 99%

Macrophage exosomes modified by miR-365-2-5p promoted osteoblast osteogenic differentiation by targeting OLFML1

Hou,

Zhang,

et al. 2024

Regenerative Biomaterials

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In the bone immune microenvironment, immune cells can regulate osteoblasts through a complex communication network. Macrophages play a central role in mediating immune osteogenesis, exosomes derived from them have osteogenic regulation and can be used as carriers in bone tissue engineering. However, there are problems with exosomal therapy alone, such as poor targeting, and the content of loaded molecules cannot reach the therapeutic concentration. In this study, macrophage-derived exosomes modified with miR-365-2-5p were developed to accelerate bone healing. MC3T3-E1 cells were incubated with the culture supernatants of M0, M1 and M2 macrophages, and it was found that the culture medium of M2 macrophages had the most significant effects in contributing to osteogenesis. High-throughput sequencing identified that miR-365-2-5p was significantly expressed in exosomes derived from M2 macrophages. We incubated MC3T3-E1 with exosomes overexpressing or knocking down miR-365-2-5p to examine the biological function of exosome miR-365-2-5p on MC3T3-E1 differentiation. This findings suggested that miR-365-2-5p secreted by exosomes increased the osteogenesis of MC3T3-E1. Moreover, miR-365-2-5p had a direct influence over osteogenesis for MC3T3-E1. Sequencing analysis combined with dual luciferase detection indicated that miR-365-2-5p binded to the 3'UTR of OLFML1. In summary, exosomes secreted by M2 macrophages targeted OLFML1 through miR-365-2-5p to facilitate osteogenesis.

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“…The observation of 22 overlapping proteins across the two examined time points of 7 and 14 days within the proteomics enrichment analysis provides significant insight into the persistent molecular mechanisms influenced by treatment with SPMs in the context of osteogenesis and osteoclastogenesis. Among these, a consistent set of 12 proteins associated with osteogenesis were identified across all samples, including ALB [ 35 ], ITGB1 [ 36 ], VIM [ 37 ], MSN [ 38 ], MYH10 [ 39 ], HADHA [ 40 ], GSS [ 41 ], COPG1 [ 42 ], SAMHD1 [ 43 ], DDX1 [ 44 ], PSMD9 [ 45 ], and MFGE8 [ 46 ]. Conversely, a subset of 10 proteins consistently implicated in osteoclastogenesis at both time points were identified, namely CDC37 [ 47 ], GNAT3 [ 48 ], PSAP [ 49 ], HMGB1 [ 50 ], CD59 [ 51 ], YBX3 [ 52 ] H3C1 [ 53 ], NLRP9 [ 54 ], CHMP5 [ 55 ], and DYNLL2 [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Resolvin-E1 and Maresin-1 on Bone Marrow Stem Cell Osteogenesis under Inflammatory Stress

AlZahrani,

Shinwari,

Alaiya

et al. 2024

Cells

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Periodontal disease is characterized by inflammation and bone loss. Central to its pathogenesis is the dysregulated inflammatory response, complicating regenerative therapies. Mesenchymal stem cells (MSCs) hold significant promise in tissue repair and regeneration. This study investigated the effects of specialized pro-resolving mediators (SPMs), Resolvin E1 (RvE1) and Maresin 1 (MaR1), on the osteogenic differentiation of human bone marrow-derived MSCs under inflammatory conditions. The stem cells were treated with SPMs in the presence of lipopolysaccharide (LPS) to simulate an inflammatory environment. Osteogenic differentiation was assessed through alkaline phosphatase activity and alizarin red staining. Proteomic analysis was conducted to characterize the protein expression profile changes, focusing on proteins related to osteogenesis and osteoclastogenesis. Treatment with RvE1 and MaR1, both individually and in combination, significantly enhanced calcified deposit formation. Proteomic analysis revealed the differential expression of proteins associated with osteogenesis and osteoclastogenesis, highlighting the modulatory impact of SPMs on bone metabolism. RvE1 and MaR1 promote osteogenic differentiation of hBMMSCs in an inflammatory environment, with their combined application yielding synergistic effects. This study provides insights into the therapeutic potential of SPMs in enhancing bone regeneration, suggesting a promising avenue for developing regenerative therapies for periodontal disease and other conditions characterized by inflammation-induced bone loss.

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M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

Cited by 5 publications

References 45 publications

Exosomes derived from M2 macrophages prevent steroid-induced osteonecrosis of the femoral head by modulating inflammation, promoting bone formation and inhibiting bone resorption

Exosomes derived from M2 macrophages prevent steroid-induced osteonecrosis of the femoral head by modulating inflammation, promoting bone formation and inhibiting bone resorption

Macrophage exosomes modified by miR-365-2-5p promoted osteoblast osteogenic differentiation by targeting OLFML1

Impact of Resolvin-E1 and Maresin-1 on Bone Marrow Stem Cell Osteogenesis under Inflammatory Stress

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