M6: A Diploid Potato Inbred Line for Use in Breeding and Genetics Research

Jansky, Shelley; Chung, Yong Suk; Kittipadukal, P.

doi:10.3198/jpr2013.05.0024crg

Cited by 82 publications

(74 citation statements)

References 37 publications

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“…Previous work focusing on the creation of homozygous inbred lines in diploid potato was limited by self‐incompatibility in diploid germplasm (Hawkes, ; Cipar et al ., ). However, the presence of a dominant allele for the S‐locus inhibitor gene Sli (Hosaka and Hanneman, ) enabled inbreeding and the development of the inbred M6 S. chacoense clone (Jansky et al ., ). Interestingly, although the M6 clone has been inbred for seven generations it still exhibits considerable residual heterozygosity, suggesting the possibility that lethal or deleterious alleles could be maintained in repulsion with beneficial alleles (Jansky et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…However, the presence of a dominant allele for the S‐locus inhibitor gene Sli (Hosaka and Hanneman, ) enabled inbreeding and the development of the inbred M6 S. chacoense clone (Jansky et al ., ). Interestingly, although the M6 clone has been inbred for seven generations it still exhibits considerable residual heterozygosity, suggesting the possibility that lethal or deleterious alleles could be maintained in repulsion with beneficial alleles (Jansky et al ., ). The M6 clone has desirable agronomic traits such as high dry matter, good chip‐processing qualities and disease resistance.…”

Section: Introductionmentioning

confidence: 97%

“…Unlike major cereal and oilseed crops, cultivated potato is a vegetatively propagated heterozygous autotetraploid. Breeding gains in potato are limited by polyploidy, inbreeding depression, low sexual fertility, low recombination and poor adaptation of wild germplasm (The Potato Genome Sequencing Consortium, ; Jansky et al ., ; Hardigan et al ., ). The creation of diploid inbred lines represents a new strategy for overcoming the challenges with historical tetraploid potato breeding approaches (Birhman and Hosaka, ; Phumichai et al ., ; Jansky et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Genome sequence of M6, a diploid inbred clone of the high‐glycoalkaloid‐producing tuber‐bearing potato species Solanum chacoense, reveals residual heterozygosity

et al. 2018

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Cultivated potato (Solanum tuberosum L.) is a highly heterozygous autotetraploid that presents challenges in genome analyses and breeding. Wild potato species serve as a resource for the introgression of important agronomic traits into cultivated potato. One key species is Solanum chacoense and the diploid, inbred clone M6, which is self-compatible and has desirable tuber market quality and disease resistance traits. Sequencing and assembly of the genome of the M6 clone of S. chacoense generated an assembly of 825 767 562 bp in 8260 scaffolds with an N50 scaffold size of 713 602 bp. Pseudomolecule construction anchored 508 Mb of the genome assembly into 12 chromosomes. Genome annotation yielded 49 124 high-confidence gene models representing 37 740 genes. Comparative analyses of the M6 genome with six other Solanaceae species revealed a core set of 158 367 Solanaceae genes and 1897 genes unique to three potato species. Analysis of single nucleotide polymorphisms across the M6 genome revealed enhanced residual heterozygosity on chromosomes 4, 8 and 9 relative to the other chromosomes. Access to the M6 genome provides a resource for identification of key genes for important agronomic traits and aids in genome-enabled development of inbred diploid potatoes with the potential to accelerate potato breeding.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Genome sequence of M6, a diploid inbred clone of the high‐glycoalkaloid‐producing tuber‐bearing potato species Solanum chacoense, reveals residual heterozygosity

et al. 2018

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“…Due to its homozygosity and self-compatibility properties, M6 could be a suitable candidate plant for all molecular works that can be extrapolated to the domesticated cultivars. At the same time, M6 possesses desired qualities in chip processing and resistance against diseases (Jansky et al, 2014;Enciso-Rodriguez et al, 2019). However, the problem of high glycoalkaloid content (Leisner et al, 2018) in M6 tubers can be addressed via breeding programs and/or transgenic/gene editing analyses.…”

Section: Discussionmentioning

confidence: 99%

Manipulating genome of diploid potato inbred line Solanumchacoense M6 using selectable marker gene

Dangol¹,

Yel²,

Çalışkan³

et al. 2020

Turk J Agric For

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The development of transgenic potatoes is imperative to investigate various gene functions as well as to develop robust potato varieties resistant to different biotic and abiotic stresses. Directing a potato breeding program from cultivated tetraploids to selfcompatible diploid lines will be highly helpful for potato breeders. However, diploid potatoes are considered recalcitrant to regeneration. We aimed to develop a protocol for the transformation of diploid Solanum chacoense M6 potatoes using leaf, internodes, and microtubers as the explants. Agrobacterium tumefaciens strain GV2260 harboring pBIN19 expression vector containing gusA gene (interrupted by an intron to induce expression from eukaryotic cells) was used for this purpose. Different inoculation times (10, 20, 30 min) were applied in aforementioned explants for gene transfer. After cocultivation, explants were transferred to the media with various hormone concentrations [6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), trans-zeatin, kinetin, and 2, 4-Dichlorophenoxyacetic acid (2,4-D)]. The calli generated were then transferred to shoot generating medium supplemented with thidiazuron (TDZ) and gibberellic acid (GA 3 ). According to histochemical GUS analysis, we found a 20 min inoculation time to be optimal for gene transfer and the medium containing 2 mg L -1 BAP and 2 mg L -1 NAA was the most suitable medium for callus induction from 20 min inoculated explants (41% callus formation for internodes and 65% for leaf explants). Abundant transcripts levels of gusA gene was found in primary transformants when subjected to RT-qPCR. GUS fluorometric assay further confirmed the primary transformants at protein level. The present study can serve as a gateway to transfer gene(s) of interest in diploid potatoes.

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“…In addition to having potential implications for understanding the GSI mechanism , Sli was soon perceived as a useful "tool" for selfing diploid potato to develop highly homozygous and seedpropagated lines (Phumichai et al, 2005). Thus, Jansky et al (2014) developed M6, a vigorous diploid SC S. chacoense line that displays 90% homozygosity (including Sli). M6 is fully fertile, produces seeds when crossed with cultivated or wild potatoes, and will enable systematic creation of inbred diploid lines.…”

Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

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Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self-and non-self pollen. Since this trait promotes outcrossing and avoids inbreeding it is a widespread mechanism of controlling sexual plant reproduction. Growers and breeders have effectively exploited SI as a tool for manipulating domesticated crops for thousands of years. However, only within the past thirty years have studies begun to elucidate the underlying molecular features of SI. The specific S-determinants and some modifier factors controlling SI have been identified in the sporophytic system exhibited by Brassica species and in the two very distinct gametophytic systems present in Papaveraceae on one side and in Solanaceae, Rosaceae, and Plantaginaceae on the other. Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. Applications of self-(in)compatibility include widely differing objectives such as crop yield and quality improvement, marker-assisted breeding through SI genotyping, and development of hybrids for overcoming intra-and interspecific reproductive barriers. Here, we review scientific progress as well as patented applications of SI, and also highlight future prospects including further elucidation of SI systems, deepening our understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.

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M6: A Diploid Potato Inbred Line for Use in Breeding and Genetics Research

Cited by 82 publications

References 37 publications

Genome sequence of M6, a diploid inbred clone of the high‐glycoalkaloid‐producing tuber‐bearing potato species Solanum chacoense, reveals residual heterozygosity

Genome sequence of M6, a diploid inbred clone of the high‐glycoalkaloid‐producing tuber‐bearing potato species Solanum chacoense, reveals residual heterozygosity

Manipulating genome of diploid potato inbred line Solanumchacoense M6 using selectable marker gene

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

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