M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT

Huang, Guangzhao; Wu, Qimeng; Zheng, Zenan; Shao, Ting-ru; Chen, Yue-chuan; Zeng, Weisen; Lv, Xiaoyang

doi:10.18632/aging.103333

Cited by 67 publications

(56 citation statements)

References 27 publications

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“…Only recently the expression profiles of 13 m6A-related genes in 317 OSCC and 32 normal samples from The Cancer Genome Atlas (TCGA) database had been analyzed and found a significantly higher expression of eight genes, including METTL3 and METTL14, in tumor tissues. Furthermore, an increase of m6A levels in total tumor RNA could be detected using an antibody-based quantification kit [ 175 ]. The upregulation of METTL3 in oral cancer was also seen in two additional studies that also report a correlation of METTL3 expression with poor prognosis of OSCC patients [ 176 , 177 ].…”

Section: Rbps Associated With Migration Invasion and Metastasis mentioning

confidence: 99%

RNA-Binding Proteins as Regulators of Migration, Invasion and Metastasis in Oral Squamous Cell Carcinoma

Weiße

Rosemann

Krauspe

et al. 2020

IJMS

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Nearly 7.5% of all human protein-coding genes have been assigned to the class of RNA-binding proteins (RBPs), and over the past decade, RBPs have been increasingly recognized as important regulators of molecular and cellular homeostasis. RBPs regulate the post-transcriptional processing of their target RNAs, i.e., alternative splicing, polyadenylation, stability and turnover, localization, or translation as well as editing and chemical modification, thereby tuning gene expression programs of diverse cellular processes such as cell survival and malignant spread. Importantly, metastases are the major cause of cancer-associated deaths in general, and particularly in oral cancers, which account for 2% of the global cancer mortality. However, the roles and architecture of RBPs and RBP-controlled expression networks during the diverse steps of the metastatic cascade are only incompletely understood. In this review, we will offer a brief overview about RBPs and their general contribution to post-transcriptional regulation of gene expression. Subsequently, we will highlight selected examples of RBPs that have been shown to play a role in oral cancer cell migration, invasion, and metastasis. Last but not least, we will present targeting strategies that have been developed to interfere with the function of some of these RBPs.

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Section: Rbps Associated With Migration Invasion and Metastasis mentioning

confidence: 99%

RNA-Binding Proteins as Regulators of Migration, Invasion and Metastasis in Oral Squamous Cell Carcinoma

Weiße

Rosemann

Krauspe

et al. 2020

IJMS

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“…In recent years, EMT has been believed to be a key step in the invasion and metastasis of cancer cells [ 39 ]. Previous studies have shown that several hnRNP family members are implicated in the regulation of EMT in several human cancer types and are regarded as cancer metastasis suppressors [ 17 , 18 , 20 , 40 , 41 ]. We investigated the function of HNRNPU in regulating EMT.…”

Section: Discussionmentioning

confidence: 99%

Identification of therapeutic targets and prognostic biomarkers from the hnRNP family in invasive breast carcinoma

Guo

Huo

Xing

et al. 2021

Aging

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Heterogeneous nuclear ribonucleoproteins (hnRNPs) are RNA-binding proteins that are reported to play a crucial role in the pathogenic process of multiple malignancies. However, their expression patterns, clinical application significance and prognostic values in invasive breast carcinoma (BRCA) remain unknown. In this study, we investigated hnRNP family members in BRCA using accumulated data from Oncomine 4.5, UALCAN Web portal and other available databases. We explored the expression and prognostic value level of hnRNPs in BRCA. We further analyzed their association with the clinicopathological features of BRCA patients. Subsequently, we calculated the alteration frequency of hnRNPs, constructed the interaction network of hnRNPs, and examined the potential coexpression genes of hnRNPs, revealing that HNRNPU and SYNCRIP are the core molecular genes requiring further investigation for BRCA. We validated the immunohistochemistry (IHC) pattern to simulate clinical applications based on pathology. Cell function experiments conducted in vitro indicated that HNRNPU can promote epithelial-mesenchymal transition, functionally stimulating the invasion capacity and inhibiting the viability of invasive BRCA cells. In summary, our systematic analysis demonstrated that HNRNPU was the key molecule that played a fundamental role in BRCA metastasis, which may facilitate the development of new diagnostic and prognostic markers for the analysis of BRCA progression.

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“…Among them, HNRNPC can regulate the stability and translation levels of bound mRNA molecules [ 38 ], while M6A can promote the binding of mRNAs and HNRNPC through the "m6A-switch" mechanism, thus regulating mRNA splicing and affecting the expression of the corresponding proteins [ 39 ]. Huang et al found that HNRNPC can promote cell proliferation, migration, and invasion [ 40 ], which was closely related to the occurrence and progression of cancer and was an important M6A regulatory factor that caused poor prognosis of patients. YTHDF1 can recognize and bind the M6A methylation of mRNA, thus inhibiting the translation of mRNA.…”

Section: Discussionmentioning

confidence: 99%

Gene signatures of 6-methyladenine regulators in women with lung adenocarcinoma and development of a risk scoring system: a retrospective study using the cancer genome atlas database

Gao

Zhuang

et al. 2021

Aging

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Although the emergence of new treatments has improved the prognosis of women with lung adenocarcinoma (LUAD), the emergence of drug resistance limits their clinical efficacy. Therefore, there is an urgent need to identify new targets and develop a risk scoring system to evaluate the prognosis of patients. 6-methyladenine (M6A), as the most common methyl modification in RNA modification, its clinicopathological features, diagnosis and prognostic value in lung cancer, especially in LUAD remain to be discussed. We analyzed the clinical and sequencing data of the female LUAD cohort from The Cancer Genome Atlas (TCGA), evaluated the expression profiles of 16 M6A regulation-related genes in the cohort and the relationships between genetic changes and clinical characteristics, developed an M6A-related risk scoring system using Cox analysis. Finally, the copy number variations (CNVs) of the related genes in the samples were analyzed and verified using the cBioPortal platform. Compared with other clinical factors, this risk scoring system showed a higher predictive sensitivity and specificity. The M6A-related risk scoring system developed in this study may help to improve the screening of female patients at high risk of LUAD and provides important theoretical bioinformatics support for evaluating the prognosis of such patients.

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M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT

Cited by 67 publications

References 27 publications

RNA-Binding Proteins as Regulators of Migration, Invasion and Metastasis in Oral Squamous Cell Carcinoma

RNA-Binding Proteins as Regulators of Migration, Invasion and Metastasis in Oral Squamous Cell Carcinoma

Identification of therapeutic targets and prognostic biomarkers from the hnRNP family in invasive breast carcinoma

Gene signatures of 6-methyladenine regulators in women with lung adenocarcinoma and development of a risk scoring system: a retrospective study using the cancer genome atlas database

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