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Okuno, Eiko; Muto, Shizuko

doi:10.5264/eiyogakuzashi.37.117

Cited by 1 publication

(4 citation statements)

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“…Glucagon (22)(23)(24)(25)(26)(27)(28)(29) and angiotensin III were purchased from Bachem Inc. (Torrance, CA), Ser-ne-Gly-Ser-Leu-Ala-Lys was from Sigma Chemical Co. (St. Louis, MO). Synthetic Glu-Glu-Leu-Cys-Thr-Met-Phe-Ile-Arg and Gly-Phe-Leu-Cys-Gly-His-Tyr-Arg (prepared using standard procedures) were kindly provided by Drs.…”

Section: Methodsmentioning

confidence: 99%

“…A product ion spectrum acquired in this manner therefore represents the combined result of high-energy CAD and low-energy metastable processes. The contributions of the fragmentations occurring inside and outside the collision cell can be separated by applying a potential to the gas collision cell [24][25][26]. When the collision cell is at ground potential, the kinetic energy E 2 of a product ion of mass m 1 formed at any point in the second field-free region is given by eq I (assuming negligible kinetic energy losses), (1) where Va is the accelerating voltage of the ion source, m 1 the mass of the precursor ion, z the charge number of the precursor ion, and e the charge of one electron.…”

mentioning

confidence: 99%

“…MIKES analyses with a potential applied to the gas collision cell were initially performed using doublefocusing instruments to study specific CAD processes (see, for example, refs [24][25][26][27][28][29]. Floating the gas collision cell was subsequently shown to improve resolution of product ions [18] and increase the transmission efficiency of low-mass (relative to the precursor) product ions transmitted through the electric sector analyzer [30].…”

mentioning

confidence: 99%

“…The CAD/MIKES analyses of glucagon (22)(23)(24)(25)(26)(27)(28)(29), Phe-Val-Gln-Trp-Leu-Met-Asn-Thr, produced abundant btl ions. Comparison of the spectra obtained with the grounded and floated collision cell showed that the btl ions possessed the same kinetic energy in both cases, consistent with the conclusion from the angiotensin III results that product ions of this type result from low-energy processes.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer

Burlet

Orkiszewski

Gaskell

1993

J. Am. Soc. Mass Spectrom.

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A hybrid tandem instrument of BEqQ geometry was used to determine high-energy decomposition of protonated peptides, such as side-chain fragmentation yielding d n and w n ions. The transmission through both E and Q of such product ions, formed in the second field-free region, permits improved mass resolution and confident mass assignment. The experimental technique may involve synchronous scanning of E and Q, or, for the purpose of identification of specific products, limited-range scanning of either E or Q with the other analyzer fixed. These techniques are not equivalent, with respect to product ion transmission, to the double focusing of product ions achieved with four-sector instruments but nevertheless represent a critical improvement over conventional mass-analyzed ion kinetic energy spectrometry analyses. Fragmentation of protonated peptides occurring in the second field-free region inside and outside the collision cell were distinguished by floating the collision cell above ground potential. Mass filtering using Q confirmed the mass assignments. The data indicate that product ions resulting from spontaneous decomposition are in some instances quantitatively more significant than those resulting from high-energy collisional activation. Furthermore, the differentiation of the products of low- and high-energy processes should facilitate spectral interpretation.

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