Machaerium acutifolium lectin inhibits inflammatory responses through cytokine modulation

Santos, Ana L.E.; Júnior, Cícero P.S.; Neto, Roberval Nascimento Moraes; Santos, Maria H.C.; Santos, Valdenice F.; Rocha, B.A.M.; Sousa, Eduardo Martins de; Carvalho, R. R. de; Menezes, Irwin Rose Alencar de; Oliveira, Maria Rayane Correia de; Dantas, Lindaiane Bezerra Rodrigues; Silva, Tarcísio M.; Teixeira, Claudener S.

doi:10.1016/j.procbio.2020.06.012

Cited by 9 publications

(7 citation statements)

References 56 publications

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“…Paw edema was induced by injecting 0.05 mL of 1% ( w / v ) carrageenan suspended in saline into the subplantar tissue of the right hind paw of each mouse [ 62 ]. A digital caliper (ZAAS Amatools, São Paulo, Brazil) was used to measure the paw thickness (Ct).…”

Section: Methodsmentioning

confidence: 99%

Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense

et al. 2022

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Mycobacterium abscessus subsp. massiliense (Mabs) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate fraction of Bixa orellana leaves (BoEA) and ellagic acid (ElAc). In silico analysis predicted that ElAc had low toxicity, was not mutagenic or carcinogenic, and had antimicrobial and anti-inflammatory activities. Apparently, ElAc can interact with COX2 and Dihydrofolate reductase (DHFR) enzymes, which could explain both activities. In vitro analysis showed that BoEA and ElAc exerted antimicrobial activity against Mabs (minimum inhibitory concentration of 1.56, 1.56 mg/mL and bactericidal concentration of 6.25, 3.12 mg/mL, respectively. Clarithromycin showed MIC and MBC of 1 and 6 µg/mL). Treatment with BoEA or ElAc increased survival of Tenebrio molitor larvae after lethal infection with Mabs and reduced carrageenan-induced paw edema in mice, around 40% of edema volume after the fourth hour, similarly to diclofenac. In conclusion, BoEA and ElAc exert antimicrobial effects against Mabs and have anti-inflammatory effects, making them potential sources of antimycobacterial drugs. The biological activities of ElAc may be due to its high binding affinities predicted for COX2 and DHFR enzymes.

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Section: Methodsmentioning

confidence: 99%

Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense

et al. 2022

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“…It was also suggested that the effect depended on the inhibition of 5-HT, BK, prostaglandin E2, nitric oxide, TNF-α, and leukocyte rolling and adhesion. Santos et al [49] demonstrated that the Machaerium acutifolium seed lectin (MaL) significantly decreased inflammation in the formalin test, inhibited cell migration in carrageenan-induced peritonitis, and blocked the formation of paw edema induced by carrageenan and dextran. In vitro studies in LPS-stimulated macrophages showed that MaL downregulated gene expression of pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS) and TNFα, while upregulated the anti-inflammatory IL-10 gene.…”

Section: Anti-inflammatory and Antinociceptive Activities Of Iectinsmentioning

confidence: 99%

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

Lima

Patriota

Albuquerque

et al. 2020

AIR

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Depression and anxiety disorder are the most common mental disorders worldwide and their treatments are combinations of pharmacological and psychotherapeutic approaches. Depression treatment depends largely on a pharmacotherapy that improves the transmission of monoamines in the brain. However, the drugs available have adverse reactions and do not contemplate positively all patients, which stimulates scientific research that seeks new molecules, including from natural sources. Lectins are proteins capable of binding reversibly and non-covalently to specific sugars. For example, it has been reported the antimicrobial, antitumor, antiparasitic, anti-inflammatory and antinociceptive activities of lectins. The ability of some lectins in modulating nociception and inflammation stimulated studies on the possible effects on processes that share some pathways and molecular agents, like depression and anxiety. Lectins isolated from plants showed antidepressant effects, which were demonstrated to be linked to activation of serotonergic, adrenergic, and dopaminergic systems as well as to inhibition of the glutamatergic system and L-arginine–NO–cGMP pathway. In view of their immunomodulatory properties, it is also suggested that lectins can ameliorate the inflammatory framework associated with depression. Anxiolytic effects were also reported and associated with modulation of GABAergic mechanisms, serotonergic system, and NO pathway. It should be taken in account that some lectins induced depressive-like behavior, associated with an neuroinflammatory action, as well anxiogenic action. Thus, it is important to use combinations of batteries for testing anxiety, depression, despair, and anhedonia behaviors in the studies with lectins. The mechanisms by which lectins exactly modulate depression or anxiety frameworks are still unclear but important windows had already been open by researchers and preclinical studies with lectins have indicated these proteins as candidates for alternative or complementary agents in therapies of depression and anxiety disorder.

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“…Inflammation is a defensive process in animal cells that usually occurs in response to injuries or pathogenic infections [13]. Inflammatory response usually results in tissue repair; however, a prolonged inflammatory process may result in chronic diseases and organ damage [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the adverse effects of synthetic anti-inflammatory drugs caused by prolonged usage, the search for anti-inflammatory agents from natural sources is of paramount interest. Plant lectins are recently being explored in the study of inflammation, and they have been discovered to act either as an anti-inflammatory or pro-inflammatory agent [14].…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive effects of Treculia africana decne (african breadfruit) seed lectin in Wistar rats

Awe

Osukoya

Adewale

et al. 2022

SR: PS

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The aim: The use of synthetic compounds to treat many diseases must be strictly controlled due to their potential health hazards. Hence, there is a need to search for natural products to serve as safe alternatives to synthetic products. This study investigated the antinociceptive effects and anti-inflammatory activities of Treculia africana seed lectin. Materials and methods: Lectins were purified from Treculia africana seeds using ion exchange and size-exclusion chromatography. The antinociceptive activity of the lectin was assessed in Wistar rats using abdominal writhing and paw-licking tests induced by acetic acid and formalin, respectively. Anti-inflammatory activity was assessed using carrageenan-induced paw oedema. Results: Treculia africana seed lectins at 10 mg/kg (p.o.) produced sedation, reduced ambulation, reduced response to touch, analgesia, and decreased defecation in experimental animals. Administration of Treculia africana seed lectin (1 mg/kg and 10 mg/kg) in experimental animals significantly reduced (P < 0.05) acetic acid-induced muscular writhing in a dose-dependent manner with 23.88 and 36.80 per cent inhibition, respectively. Both early and late phases of formalin-induced nociception were significantly inhibited (P < 0.001) by the lectin at all doses (0.1, 1.0 and 10.0 mg/kg), comparably with the standard drug, diclofenac sodium. At 10 mg/kg, T. africana lectin caused a 69.12 % and 65.55 % reduction in both early and late phases of formalin-induced paw licking. Treculia africana lectin also significantly brought about a reduction (P < 0.05) in inflammation induced by sub-plantar injection of carrageenan as measured by a decrease in paw swollenness. Conclusion: The study showed that Treculia africana lectin possesses antinociceptive and anti-inflammatory properties and can potentially be employed therapeutics to ameliorate pain and inflammation

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Machaerium acutifolium lectin inhibits inflammatory responses through cytokine modulation

Cited by 9 publications

References 56 publications

Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense

Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense

Can Plant Lectins be Alternatives to Treat Anxiety and Depressive Disorders?

Antinociceptive effects of Treculia africana decne (african breadfruit) seed lectin in Wistar rats

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