2020
DOI: 10.1101/2020.12.18.423496
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Machine Leaning-based Determination of Sampling Depth for Complex Environmental Systems: Case Study with Single-Cell Raman Spectroscopy Data in EBPR Systems

Abstract: Rapid progress in various advanced analytical methods such as single-cell technologies enable unprecedented and deeper understanding of microbial ecology beyond the resolution of conventional approaches. A major application challenge exists in the determination of sufficient sample size without sufficient prior knowledge of the community complexity and, the need to balance between statistical power and limited time or resources. This hinders the desired standardization and wider application of these technologi… Show more

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Cited by 3 publications
(2 citation statements)
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“…The relative intensity of poly-P for single cell was normalized by the intensity of amide I vibration at 1640-1690 cm -1 . For effective analysis, sufficient (78-137) bacterial Raman spectrum were collected according to Li et al, (2022).…”
Section: Single Cell Raman Spectrum (Scrs) Analysis and Phenotypic Pr...mentioning
confidence: 99%
“…The relative intensity of poly-P for single cell was normalized by the intensity of amide I vibration at 1640-1690 cm -1 . For effective analysis, sufficient (78-137) bacterial Raman spectrum were collected according to Li et al, (2022).…”
Section: Single Cell Raman Spectrum (Scrs) Analysis and Phenotypic Pr...mentioning
confidence: 99%
“…Sampling sizes for this study were considered sufficient based on our statistical evaluation method by Li et al (2020). All Raman spectra were processed with cosmic spike removal, background subtraction, and baseline correction using LabSpec 6 software (Horiba Jobin Yvon, Kyoto, Japan).…”
Section: Scrs Analysis and Data Processingmentioning
confidence: 99%