Machine Learning Algorithms for a Novel Cuproptosis-related Gene Signature of Diagnostic and Immune Infiltration in Endometriosis

Abstract

Endometriosis (EMT) is a chronic hormone-dependent disease where in viable endometrial tissue is transplanted outside the uterus. Interestingly, immune infiltration is significantly involved in EMT pathogenesis. Currently, no studies have shown the involvement of cuproptosis-related genes (CRGs) in regulating immune infiltration in EMT. This study identified three CRGs such as GLS, NFE2L2, and PDHA1, associated with EMT using machine learning algorithms. These three CRGs were upregulated in the endometrium of patients with moderate/severe EMT and downregulated in patients with infertility. Single sample genomic enrichment analysis (ssGSEA) revealed that these CRGs were closely correlated with autoimmune diseases such as systemic lupus erythematosus. Furthermore, these CRGs were correlated with immune cells such as eosinophils, natural killer cells, and macrophages. Therefore, profiling patients based on these genes aid in a more accurate diagnosis of EMT progression. These findings provide a new idea for the pathology and treatment of endometriosis, suggesting that CRGs such as GLS, NFE2L2, and PDHA1 may play a key role in the occurrence and development of endometriosis.

Artificial Intelligence in the Management of Women with Endometriosis and Adenomyosis: Can Machines Ever Be Worse than Humans?

“…ML models showed a high classification performance and were able to consistently reveal a number of pollutants associated with the disease. Genetic studies analyzing the expression of long non-coding RNAs [104] and of genes [105,106] reported an up-regulation of the genes involved in vasculogenesis, cell proliferation, cell-matrix adhesion, cell differentiation, extra-cellular matrix, remodeling muscle contraction, apoptosis, immune response and chemotaxis.…”