Maclura pomifera agglutinin-binding glycoconjugates on converted apatite from synthetic octacalcium phosphate implanted into subperiosteal region of mouse calvaria

Сузукі, Осаму; Nakamura, Masanori; Miyasaka, Yoshinori; Kagayama, Manabu; Sakurai, Minoru

doi:10.1016/s0169-6009(08)80024-4

Cited by 111 publications

(155 citation statements)

References 19 publications

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“…Much attention has been paid to the usability of synthetic OCP and its predicted role in nucleation of bone induction in orthotopic sites, which can be replaced with a significantly higher volume of newly formed bone than the other calcium phosphate phases, such as amorphous carbonated apatite or HA 8) . The osteoconductivity of OCP was first found in the granule form on implantation in the subperiosteal region of murine calvaria 9,10) . The biological responses to OCP have also been investigated from its coating on metallic implants 11,12) , in osteoblastic cell proliferation, or from implantation into subcutaneous tissue or bone defects 8,12) .…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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“…Our previous studies demonstrated that the octacalcium phosphate (OCP) causes new appositional bone formation on it when implanted in the subperiosteal region of murine bone (Kamakura et al, 1996;Sasano et al, 1995;Suzuki et al, 1993), whereas no bone formation is identified on the OCP when the implant is placed on the membrane filter under the periosteum to prevent the periosteum from contacting the bone surface (Sasano et al, 1999). Based on these data we hypothesized that the physical contact of the periosteum with the bone matrix is requisite for osteoblastic differentiation from the osteogenic layer of the periosteum, which leads to the subperiosteal bone formation on the implant.…”

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confidence: 99%

Osteoblastic differentiation of periosteum‐derived cells is promoted by the physical contact with the bone matrix in vivo

et al. 2001

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The periosteum contains osteoprogenitors that differentiate to osteoblasts in bone growth or repair. Our previous studies suggested the hypothesis that the physical contact of the periosteum with the bone matrix is requisite for the differentiation of osteoblasts. To test the hypothesis, the present study was designed to investigate how the contact between the periosteum and the bone matrix influences the osteoblastic differentiation of periosteal cells with establishing a new experimental model in vivo. Differentiation of osteoblasts was assessed by gene expression of type I collagen, osteocalcin and bone sialoprotein using in situ hybridization. A barrier was designed to prevent periosteal cells from contacting the bone matrix using the membrane filter. The membrane filter was inserted surgically between the surface of rat parietal bone and the periosteum after being punched out with pin holes. Periosteal cells were allowed to contact with the bone surface only through the pin holes. The pin hole was filled with cells derived from the periosteum 1 week after inserting the filter. Differentiation of osteoblasts in week 2 and noticeable bone formation in week 3 were identified on the bone surface only under the pin hole but not under the filter. The present study demonstrated that the physical contact with the bone matrix promotes osteoblastic differentiation of periosteumderived cells in vivo. Anat Rec 264:72-81, 2001.

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“…The sieved granules (particle size of 300-500 μm) of OCP, obtained from dried OCP, were sterilized by heating at 120ºC for 2 hours. A previous study showed that such heating does not affect physical properties such as the crystalline structure or specific surface area of OCP granules [26,27]. Bone matrix gelatin (BMG) was prepared using the method previously described by urist et al [28] which was modified by the method of Wei-Qi Yan et al [3].…”

Section: A Preparation Of Materialsmentioning

confidence: 99%

Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

Aval¹,

Saberi²,

Sargolzaei³

et al. 2018

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Objective: Regeneration of bone defects remains a challenge for maxillofacial and reparative surgeons. The purpose of this histological study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination in artificially created mandibular bone defects in rats. The quality of the newly formed bone was also evaluated.Methods: Thirty-six male Sprague Dawley rats (6-8 weeks old with 120-150 g weight) were randomly divided into four groups. Defects (3 mm in diameter and 2 mm in depth) were created in the mandible of rats and filled with 6 mg of OCP, BMG or a combination of both (1/4 ratio), respectively. Defects were left unfilled in the control group. To assess osteoinduction and bone regeneration and determine the quality of the newly formed bone, tissue specimens were harvested at seven, 14, and 21 days post-implantation. The specimens were processed, stained with hematoxylin and eosin (H&E) and histologically analyzed under light microscopy. Results:In the experimental groups, new bone formation was initiated at the margins of defects from seventh day after implantation. At the end of the study period, the amount of the newly formed bone increased and the bone was relatively mature. Osteoinduction and new bone formation were greater in OCP/BMG group. In the control group, slight amount of new bone had been formed at the defect margins (next to host bone) on day 21. Conclusion:Combination of OCP/BMG may serve as an optimal biomaterial for treatment of mandibular bone defects.

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Maclura pomifera agglutinin-binding glycoconjugates on converted apatite from synthetic octacalcium phosphate implanted into subperiosteal region of mouse calvaria

Cited by 111 publications

References 19 publications

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of periosteum‐derived cells is promoted by the physical contact with the bone matrix in vivo

Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

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