The objective of this research was to determine the influence of ripening season on nutrient concentration and nutrient partitioning of peach trees (Prunus persica L. Batsch). We selected peach trees from three different ripening seasons and measured: (1) the concentration of macronutrients in pruned wood, thinned fruitlets, harvested fruit, and leaves fallen in autumn and (2) the total amount of macronutrients at each of these removal events. Our results showed that early‐season cultivars had more K in pruned wood, more P and K in fallen leaves, and more N, P, K, and Mg in mature fruits than mid‐ and late‐season cultivars. Also, early‐season cultivars removed more dry weight from pruned wood and fallen leaves but less from fruit than mid‐ and late‐season cultivars. These results suggest that different ripening season can affect peach tree nutrient concentration and nutrient partitioning and, consequently, peach cultivars harvested at different times of the year may benefit from specific fertilization programs rather than uniform, calendar‐based fertilization programs.