Macro Kinetics of Biocatalysis in Pickering Emulsions

Schumacher, Lynn C.; Plikat, Christoph; Ansorge‐Schumacher, Marion B.; Drews, Anja

doi:10.1002/cite.201450633

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“…We expected phase complexity of the cell‐stabilized PE to reduce upon modification of hydrophobicity, and thus wettability, of the cell surface. According to a method previously developed by our group, we deposited a hydrophobic silicone (derived via Pt‐catalyzed hydrosilylation from commercial siloxanes DVS‐50 and SiH 50/10; for details see the Supporting Information) on the surfaces of lyophilized E. coli SG 13009 cells containing recombinant BAL (see Figure S2). With these surface‐modified cells, considerably fewer multi‐emulsions were observed directly after formation, and after stirring at 220 rpm for up to 24 hours (Figure B; see Figure S3).…”

Section: Methodsmentioning

confidence: 99%

Efficient and Selective Carboligation with Whole‐Cell Biocatalysts in Pickering Emulsion

2019

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Pickering emulsions (PEs) are particle-stabilized multiphase systems with promising features for synthetic applications.D escribed here is an ovel, simplified set-up employing catalytically active whole cells for simultaneous emulsion stabilization and synthetic reaction. In the stereoselective carboligation of benzaldehyde to (R)-benzoin catalyzed by abenzaldehyde lyase in E. coli, the set-up yielded maximum substrate conversion within very short time,while economizing material demand and waste.F ormation and activity of freshly produced PEs were enhanced when the catalytic whole cells were covered with hydrophobic silicone prior to PE formation. Benchmarked against other easy-to-handle whole-cell biocatalysts in pure organic solvent, neat substrate,a na queous emulsion in substrate,and amicro-aquatic system, respectively, the cell-stabilized PE outperformed all other systems by far.

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Section: Methodsmentioning

confidence: 99%