Macroalgal biomass subcritical hydrolysates for the production of polyhydroxyalkanoate (PHA) by Haloferax mediterranei

Ghosh, Supratim; Gnaim, Rima; Greiserman, Semion; Fadeev, Ludmila; Gozin, Michael; Golberg, Alexander

doi:10.1016/j.biortech.2018.09.108

Cited by 113 publications

(46 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectra corresponded to the typical profile of a copolymer 3-hydroxybutyrate (3HB) co 3-hydroxyvalerate (3HV). Indeed, similar observations were previously reported [ 62 , 63 , 64 ]. Compared to the commercial sample, a higher intensity of the branched alkyl fraction (2913–2850 cm −1 ) was observed ( Figure 5 ), thus hypothesizing the higher presence of the poli-3-HV fraction in the copolymer.…”

Section: Resultssupporting

confidence: 91%

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Raho¹,

Carofiglio²,

Montemurro

et al. 2020

Foods

View full text Add to dashboard Cite

In the last decade, the dairy industry underwent a rapid expansion due to the increasing demand of milk-based products, resulting in high quantity of wastewater, i.e., whey and ricotta cheese exhausted whey (RCEW). Although containing high content of nutritional compounds, dairy by-products are still disposed as waste rather being reintroduced in a new production chain, hence leading to environmental and economic issues. This study proposes a new biotechnological approach based on the combination of membrane filtration and fermentation to produce poly-hydroxyalkanoates (PHA), biodegradable bioplastics candidate as an alternative to petroleum-derived plastics. The protocol, exploiting the metabolic capability Haloferax mediterranei to synthesize PHA from RCEW carbon sources, was set up under laboratory and pilot scale conditions. A multi-step fractionation was used to recover a RCEW fraction containing 12.6% (w/v) of lactose, then subjected to an enzymatic treatment aimed at releasing glucose and galactose. Fermentation conditions (culture medium for the microorganism propagation, inoculum size, time, and temperature of incubation) were selected according to the maximization of polymer synthesis, under in-flasks experiments. The PHA production was then tested using a bioreactor system, under stable and monitored pH, temperature, and stirring conditions. The amount of the polymer recovered corresponded to 1.18 g/L. The differential scanning calorimetry (DSC) analysis revealed the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as the polymer synthesized, with a relatively high presence of hydroxyvalerate (HV). Identity and purity of the polymer were confirmed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) and X-ray photoelectron (XPS) spectroscopy analyses. By combining the fractionation of RCEW, one of the most abundant by-products from the agri-food industry, and the use of the halophile Hfx mediterranei, the production of PHBV with high purity and low crystallinity has successfully been optimized. The process, tested up to pilot scale conditions, may be further implemented (e.g., through fed-batch systems) and used for large-scale production of bioplastics, reducing the economical and environmental issues related the RCEW disposal.

show abstract

Section: Resultssupporting

confidence: 91%

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Raho¹,

Carofiglio²,

Montemurro

et al. 2020

Foods

View full text Add to dashboard Cite

show abstract

“…was chosen as the model species as it is very common on the shores of Israel and displayed high biomass productivity in extensive cultivation offshore in Israeli waters . Furthermore, the production of proteins and starch, and biomass fermentation to acetone, ethanol, butanol, and polyhydroxyalkanoates from several Ulva species has already been demonstrated …”

Section: Introductionmentioning

confidence: 99%

Feasibility study of Ulva sp. (Chlorophyta) intensive cultivation in a coastal area of the Eastern Mediterranean Sea

Chemodanov

Robin

Jinjikhashvily

et al. 2019

Biofuels Bioprod Bioref

Self Cite

View full text Add to dashboard Cite

Increasing biomass production yields is a critical challenge for macroalgae biorefineries. The continuous tumbling and mixing of free‐floating algae through water or airflow has been shown to increase the productivity of algae in land‐based cultivation systems. This approach has not been tested thoroughly in offshore cultivation. We report, here, a field feasibility study on the increase in green macroalga Ulva sp. growth rates in offshore cages, achieved by the combined effect of tumbling and mixing of the algae using influxes of water and air. The experimental system was tested in a shallow coastal area in central Israel, in the eastern Mediterranean Sea. A maximum daily growth rate of 19.2%, areal productivity of 33.72 g dry weight (DW) day−1 m−2, and volumetric yields of 37.78 g DW day−1 m−3, together with 38.47 ± 0.01% ash and 5.28% protein content on a dry matter basis were achieved in the cages with intensified cultivation in the first week of May 2017. Our study shows that cultivation with tumbling and mixing of biomass with air, and water exchange with the environment is a feasible method to increase Ulva sp. biomass productivity offshore. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

“…mediterranei was capable to produce 55% (wt) PHBV by utilizing supercritical hydrolysate of Ulva sp. [71]. Thus, this is a double benefitted approach towards value-added polymer synthesis as well as waste remediation.…”

Section: Low-cost Substrate Usage By Haloarchaeamentioning

confidence: 99%

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

et al. 2020

View full text Add to dashboard Cite

Plastic pollution is a severe threat to our environment which necessitates implementation of bioplastics to realize sustainable development for a green world. Polyhydroxyalkanoates (PHA) represent one of the potential candidates for these bioplastics. However, a major challenge faced by PHA is the high production cost which limits its commercial application. Halophiles are considered to be a promising cell factory for PHA synthesis due to its several unique characteristics including high salinity requirement preventing microbial contamination, high intracellular osmotic pressure allowing easy cell lysis for PHA recovery, and capability to utilize wide spectrum of low-cost substrates. Optimization of fermentation parameters has made it plausible to achieve large-scale production at low cost by using halophiles. Further deeper insights into halophiles have revealed the existence of diversified and even novel PHA synthetic pathways within different halophilic species that greatly affects PHA type. Thus, precise metabolic engineering of halophiles with the help of advanced tools and strategies have led to more efficient microbial cell factory for PHA production. This review is an endeavour to summarize the various research achievements in these areas which will help the readers to understand the current developments as well as the future efforts in PHA research.

show abstract

Macroalgal biomass subcritical hydrolysates for the production of polyhydroxyalkanoate (PHA) by Haloferax mediterranei

Cited by 113 publications

References 46 publications

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Feasibility study of Ulva sp. (Chlorophyta) intensive cultivation in a coastal area of the Eastern Mediterranean Sea

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

Contact Info

Product

Resources

About