Macroautophagy involved in testosterone synthesis in Leydig cells of male dairy goat (Capra hircus)

Chen, Hong; Chen, Kexing; Zhao, Fange; Guo, Yihan; Liang, Yue; Wang, Zhengrong; Liu, Tengfei; Chen, Shulin

doi:10.1016/j.theriogenology.2021.12.023

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…2 ). Similarly, previous studies have revealed that the activities of endoplasmic reticulum and lysosome of gonad cells are varied along with sexual maturity [ 8 , 12 ]. The endoplasmic reticulum is the location of protein synthesis and assembly as well as lipid and membrane synthesis, which plays crucial roles in sperm maturation modulation [ 13 , 14 ].…”

Section: Discussionmentioning

confidence: 72%

Identification of postnatal development dependent genes and proteins in porcine epididymis

Fang,

Li,

Pang

et al. 2023

BMC Genomics

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Background The epididymis is a highly regionalized tubular organ possesses vectorial functions of sperm concentration, maturation, transport, and storage. The epididymis-expressed genes and proteins are characterized by regional and developmental dependent pattern. However, a systematic and comprehensive insight into the postnatal development dependent changes in gene and protein expressions of porcine epididymis is still lacking. Here, the RNA and protein of epididymis of Duroc pigs at different postnatal development stages were extracted by using commercial RNeasy Midi kit and extraction buffer (7 M Urea, 2 M thiourea, 3% CHAPS, and 1 mM PMSF) combined with sonication, respectively, which were further subjected to transcriptomic and proteomic profiling. Results Transcriptome analysis indicated that 198 and 163 differentially expressed genes (DEGs) were continuously up-regulated and down-regulated along with postnatal development stage changes, respectively. Most of the up-regulated DEGs linked to functions of endoplasmic reticulum and lysosome, while the down-regulated DEGs mainly related to molecular process of extracellular matrix. Moreover, the following key genes INSIG1, PGRMC1, NPC2, GBA, MMP2, MMP14, SFRP1, ELN, WNT-2, COL3A1, and SPARC were highlighted. A total of 49 differentially expressed proteins (DEPs) corresponding to postnatal development stages changes were uncovered by the proteome analysis. Several key proteins ACSL3 and ACADM, VDAC1 and VDAC2, and KNG1, SERPINB1, C3, and TF implicated in fatty acid metabolism, voltage-gated ion channel assembly, and apoptotic and immune processes were emphasized. In the integrative network, the key genes and proteins formed different clusters and showed strong interactions. Additionally, NPC2, COL3A1, C3, and VDAC1 are located at the hub position in each cluster. Conclusions The identified postnatal development dependent genes and proteins in the present study will pave the way for shedding light on the molecular basis of porcine epididymis functions and are useful for further studies on the specific regulation mechanisms responsible for epididymal sperm maturation.

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Section: Discussionmentioning

confidence: 72%

Identification of postnatal development dependent genes and proteins in porcine epididymis

Fang,

Li,

Pang

et al. 2023

BMC Genomics

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“…Furthermore, in vivo and in vitro findings by Chen et al [196] confirmed that autophagy activity is connected to steroidogenesis in the Leydig cells of dairy goats. The Leydig cells of dairy goats treated with an autophagy inhibitor (3-methyladenine; 3-MA) showed a decrease in testosterone production, while their treatment with an autophagy activator (rapamycin) resulted in the enhancement of testosterone production [196].…”

Section: Autophagy and Leydig Cell Steroidogenesismentioning

confidence: 79%

“…Recently, it has been shown that N6-methyladenosine (m6A) mRNA methylation regulates testosterone synthesis by modulating autophagy in Leydig cells [196]. m6A, the predominant internal modification in mRNA, regulates murine spermatogenesis [197].…”

Section: Autophagy and Leydig Cell Steroidogenesismentioning

confidence: 99%

Role of Macroautophagy in Mammalian Male Reproductive Physiology

Kirat

Alahwany

Arisha

et al. 2023

Cells

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Physiologically, autophagy is an evolutionarily conserved and self-degradative process in cells. Autophagy carries out normal physiological roles throughout mammalian life. Accumulating evidence shows autophagy as a mechanism for cellular growth, development, differentiation, survival, and homeostasis. In male reproductive systems, normal spermatogenesis and steroidogenesis need a balance between degradation and energy supply to preserve cellular metabolic homeostasis. The main process of autophagy includes the formation and maturation of the phagophore, autophagosome, and autolysosome. Autophagy is controlled by a group of autophagy-related genes that form the core machinery of autophagy. Three types of autophagy mechanisms have been discovered in mammalian cells: macroautophagy, microautophagy, and chaperone-mediated autophagy. Autophagy is classified as non-selective or selective. Non-selective macroautophagy randomly engulfs the cytoplasmic components in autophagosomes that are degraded by lysosomal enzymes. While selective macroautophagy precisely identifies and degrades a specific element, current findings have shown the novel functional roles of autophagy in male reproduction. It has been recognized that dysfunction in the autophagy process can be associated with male infertility. Overall, this review provides an overview of the cellular and molecular basics of autophagy and summarizes the latest findings on the key role of autophagy in mammalian male reproductive physiology.

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“…[26].The data revealed that sperm morphology was positively associated with testosterone levels. Low testosterone levels, for example, may result in small and soft testicles, as well as a decrease in the volume and function of the prostate, resulting in decreased reproductive capacity [27,28]. Additionally, there was a bad correlation between sperm DNA fragmentation and testosterone levels [22].…”

Section: Discussionmentioning

confidence: 99%

Testicular Biometry, Spermigram, and Biochemical Parameters In Male Goats

Samir,

Ali,

Mohamed

et al. 2024

Egyptian Journal of Veterinary Sciences

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HE STUDY is designed to monitor how both the age and body weight of clinically healthy male goats would be related to testicular biometry, semen attributes, and biochemical profiles. Thirty male goats (Crossbreed) aged between 2.5 and 4 years with body weight 41.2±0.83 kg (mean±SEM) were included. The morphometry of the testes and scrota of all males were measured and blood plasma samples were harvested for further analysis. The results showed that there was a strong positive correlation between the body weight of the male goats and testicular measurements; while there was a negative correlation between the body weight and scrotal length measurement. Moreover, positive correlations were found between the body weight and most of the semen parameters except semen pH, a negative correlation was found. Strong positive correlations between the age of the male goats and most of the testicular measurements were recorded. The semen volume and sperm motility were positively correlated with age, while the semen pH was negatively correlated. Moreover, semen volume, spermatozoa motility, viability, and concentration were positively correlated to testicular and scrotal measurements. In contrast, semen pH and primary abnormalities of the spermatozoa were negatively correlated with testicular and scrotal measurements. The levels of testosterone and glucose, globin, and total protein were found to be negatively correlated with spermatozoa abnormalities. In conclusion, besides the clinical findings, the scrotal and testicular measurements and biochemical analysis could be a good mirror for monitoring breeding soundness evaluation and early diagnosis of subfertile male goats

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Macroautophagy involved in testosterone synthesis in Leydig cells of male dairy goat (Capra hircus)

Cited by 9 publications

References 33 publications

Identification of postnatal development dependent genes and proteins in porcine epididymis

Identification of postnatal development dependent genes and proteins in porcine epididymis

Role of Macroautophagy in Mammalian Male Reproductive Physiology

Testicular Biometry, Spermigram, and Biochemical Parameters In Male Goats

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