Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment

Zhang, Liying; Jin, Meishu; Shi, Xian; Jin, Liming; Hou, Xiyan; Yu, Yong; Liu, Baoquan; Cao, Jijuan; Quan, Chunshan

doi:10.1007/s12010-022-03841-8

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…Biosynthesis and secretion of all these compounds by Bacillus spp. have shown direct mechanisms to suppress plant pathogens [57,58,59]. Several studies reported that the macrolide compounds macrolactin, bacillaene, and difficidin inhibit protein synthesis, impair cell division, and damage the cell membrane to restrict growth of phytopathogens [33,60].…”

Section: Discussionmentioning

confidence: 99%

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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Plant diseases are among the major factors affecting plant productivity. Biological control of plant diseases is preferred over chemical control as it is environment-friendly, cost-effective, and sustainable. Among many microbes capable of providing biological control of plant diseases, probiotic Bacillus species are most promising as they can survive in adverse conditions, provide plants with a wide range of benefits including protection from phytopathogens. Wheat blast caused by Magnaporthe oryzae Triticum pathotype (MoT) has emerged as a potential threat to global wheat production. Due to unreliability of fungicides and limited cultivar resistance, we aimed to screen and identify potential antagonist bacteria collected from internal tissues of rice and wheat seeds to determine their in vitro and in vivo inhibitory effects against MoT. Dual culture and seedling assays were performed to evaluate the efficacy of probiotic bacteria. Out of 170 bacterial isolates, three bacteria (BTS-3, BTS-4, and BTLK6A) were screened as potential antagonists against MoT in vitro. Artificial inoculation at the seedling stage showed that the isolates BTS-4, BTS–3, and BTLK6A reduced 89, 88, and 85% of wheat blast disease severity, respectively, compared to mock-inoculated control. The bacterial isolates were identified as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) through genome phylogeny. The whole genome sequence of these three bacterial strains decoded a number of orthologs to intrinsic genes of antimicrobial peptides, antioxidant defense enzymes, cell wall degrading enzymes, compounds involved in the induction of systemic resistance (ISR) in host plants, and volatile compounds to make them promising biologicals to control MoT in wheat. Combined data of in vitro and in vivo along with genome analysis suggest that Bacillus spp. suppress the destructive wheat blast disease likely through antibiosis and ISR in the host plants. Further field evaluation and characterization of antimicrobial compounds are needed for a better understanding of the mode of action and practical recommendation of these bacteria for wheat blast control in the farmers’ fields.

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Section: Discussionmentioning

confidence: 99%

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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“…Bacteria of the genus Bacillus and related genera are widely distributed in the environment, e.g., in soil, air, water, animals, plants, or sediments [50][51][52][53]. This group of bacteria plays a particularly important role in the soil, including the decay of matter [54], promotion of plant growth, and protection against phytopathogens.…”

Section: Occurrence Of Spore-forming Bacteria In Arable Soilsmentioning

confidence: 99%

Taxonomy, Ecology, and Cellulolytic Properties of the Genus Bacillus and Related Genera

Dobrzyński,

Wróbel,

Górska

2023

Agriculture

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Bacteria of the genus Bacillus and related genera (e.g., Paenibacillus, Alicyclobacillus or Brevibacillus) belong to the phylum Firmicutes. Taxonomically, it is a diverse group of bacteria that, to date, has not been well described phylogenetically. The group consists of aerobic and relatively anaerobic bacteria, capable of spore-forming. Bacillus spp. and related genera are widely distributed in the environment, with a particular role in soil. Their abundance in the agricultural environment depends mainly on fertilization, but can also depend on soil cultivated methods, meaning whether the plants are grown in monoculture or rotation systems. The highest abundance of the phylum Firmicutes is usually recorded in soil fertilized with manure. Due to the great abundance of cellulose in the environment, one of the most important physiological groups among these spore-forming bacteria are cellulolytic bacteria. Three key cellulases produced by Bacillus spp. and related genera are required for complete cellulose degradation and include endoglucanases, exoglucanases, and β-glucosidases. Due to probable independent evolution, cellulases are encoded by hundreds of genes, which results in a large structural diversity of these enzymes. The microbial degradation of cellulose depends on its type and environmental conditions such as pH, temperature, and various substances including metal ions. In addition, Bacillus spp. are among a few bacteria capable of producing multi-enzymatic protein complexes called cellulosomes. In conclusion, the taxonomy of Bacillus spp. and related bacteria needs to be reorganized based on, among other things, additional genetic markers. Also, the ecology of soil bacteria of the genus Bacillus requires additions, especially in the identification of physical and chemical parameters affecting the occurrence of the group of bacteria. Finally, it is worth adding that despite many spore-forming strains well-studied for cellulolytic activity, still few are used in industry, for instance for biodegradation or bioconversion of lignocellulosic waste into biogas or biofuel. Therefore, research aimed at optimizing the cellulolytic properties of spore-forming bacteria is needed for more efficient commercialization.

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“…The mobile phase was composed of ACN/chloroform eluted in gradient mode at Recent publications using HPLC to isolate substances derivative from marine microorganisms include yet macrolactin metabolite production by bacillus sp. zj318 isolated from marine sediment [103] and new metabolites from the marine sponge scopalina hapalia collected in Mayotte lagoon [104] (Table 2).…”

Section: Marine Microorganismsmentioning

confidence: 99%

Preparative high‐performance liquid chromatography: Isolation of natural chemical compounds for identification and characterization

Niculau

Oliveira

Almeida

2022

Separation Science Plus

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The identification and characterization of natural products generally require the isolation of pure compounds employing chromatographic techniques associated with extensive sample preparation. Preparative high-performance liquid chromatography often allows the isolation of simple and complex natural compounds by using little sample preparation or from crude extracts. Thus, this article discusses the main fundamentals of this powerful technique, as well as discusses several applications that show the isolation of natural products from plants, bacteria, fungi, and marine organisms (algae, ascidian, bacteria, sponges, and fungi).

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Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment

Cited by 10 publications

References 24 publications

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Taxonomy, Ecology, and Cellulolytic Properties of the Genus Bacillus and Related Genera

Preparative high‐performance liquid chromatography: Isolation of natural chemical compounds for identification and characterization

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