Macrolide resistance genes and mobile genetic elements in waterways from pig farms to the sea in Taiwan

Kadoya, Aya; Masuda, Nagi; Sugimoto, Yuta; Takada, Hideshige; Mizukawa, Kaoruko; Takei, Ayaka; Chou, Hsin-Yiu; Wu, Jer Horng

doi:10.1016/j.jgar.2022.04.024

Cited by 14 publications

(1 citation statement)

References 40 publications

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“…1 A, the control group (fed on milk not supplemented with bacteria) was negative for traI throughout the experimental time course. This observation was important: while pAQU1 originally was detected in marine bacteria [ 20 , 25 ], a recent study detected pAQU1 in wastewater obtained from a pig farm in Taiwan [ 26 ], suggesting that this plasmid, while still not abundant, might be spreading in the environment. The present result confirmed that the house flies used in the present study do not carry this plasmid (as assessed by traI ) as part of their native intestinal microbiome.…”

Section: Resultsmentioning

confidence: 99%

Persistence of Marine Bacterial Plasmid in the House Fly (Musca domestica): Marine-Derived Antimicrobial Resistance Genes Have a Chance of Invading the Human Environment

Nawata,

Kadoya,

Suzuki

2024

Microb Ecol

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The house fly is known to be a vector of antibiotic-resistant bacteria (ARB) in animal farms. It is also possible that the house fly contributes to the spread of ARB and antibiotic resistance genes (ARGs) among various environments. We hypothesized that ARB and ARGs present in marine fish and fishery food may gain access to humans via the house fly. We show herein that pAQU1, a marine bacterial ARG-bearing plasmid, persists in the house fly intestine for 5 days after fly ingestion of marine bacteria. In the case of Escherichia coli bearing the same plasmid, the persistence period exceeded 7 days. This interval is sufficient for transmission to human environments, meaning that the house fly is capable of serving as a vector of marine-derived ARGs. Time course monitoring of the house fly intestinal microflora showed that the initial microflora was occupied abundantly with Enterobacteriaceae. Experimentally ingested bacteria dominated the intestinal environment immediately following ingestion; however, after 72 h, the intestinal microflora recovered to resemble that observed at baseline, when diverse genera of Enterobacteriaceae were seen. Given that pAQU1 in marine bacteria and E. coli were detected in fly excrement (defined here as any combination of feces and regurgitated material) at 7 days post-bacterial ingestion, we hypothesize that the house fly may serve as a vector for transmission of ARGs from marine items and fish to humans via contamination with fly excrement.

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Section: Resultsmentioning

confidence: 99%

Persistence of Marine Bacterial Plasmid in the House Fly (Musca domestica): Marine-Derived Antimicrobial Resistance Genes Have a Chance of Invading the Human Environment

Nawata,

Kadoya,

Suzuki

2024

Microb Ecol

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Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater

Raju,

Ansari,

Patil

et al. 2024

Journal of Basic Microbiology

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Antibiotic resistance is one of the major health threat for humans, animals, and the environment, according to the World Health Organization (WHO) and the Global Antibiotic‐Resistance Surveillance System (GLASS). In the last several years, wastewater/sewage has been identified as potential hotspots for the dissemination of antibiotic resistance and transfer of resistance genes. However, systematic approaches for mapping the antibiotic resistance situation in sewage are limited and underdeveloped. The present review has highlighted all possible perspectives by which the dynamics of ARBs/ARGs in the environment may be tracked, quantified and assessed spatio‐temporally through surveillance of wastewater. Moreover, application of advanced methods like wastewater metagenomics for determining the community distribution of resistance at large has appeared to be promising. In addition, monitoring wastewater for antibiotic pollution at various levels, may serve as an early warning system and enable policymakers to take timely measures and build infrastructure to mitigate health crises. Thus, by understanding the alarming presence of antibiotic resistance in wastewater, effective action plans may be developed to address this global health challenge and its associated environmental risks.

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The β-Lactamase Activity at the Community Level Confers β-Lactam Resistance to Bloom-Forming Microcystis aeruginosa Cells

Park,

Kim,

Kim

et al. 2023

J Microbiol.

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Macrolide resistance genes and mobile genetic elements in waterways from pig farms to the sea in Taiwan

Cited by 14 publications

References 40 publications

Persistence of Marine Bacterial Plasmid in the House Fly (Musca domestica): Marine-Derived Antimicrobial Resistance Genes Have a Chance of Invading the Human Environment

Persistence of Marine Bacterial Plasmid in the House Fly (Musca domestica): Marine-Derived Antimicrobial Resistance Genes Have a Chance of Invading the Human Environment

Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater

The β-Lactamase Activity at the Community Level Confers β-Lactam Resistance to Bloom-Forming Microcystis aeruginosa Cells

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