Macromolecular Components of the Luminal Fluid From the Bovine Uterus

Roberts, G P; Parker, Janice

doi:10.1530/jrf.0.0400291

Cited by 67 publications

(41 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Roberts & Parker (1976) Harrison, Heap, Horton & Poyser (1972) showed that surgical separation of the uterus and the ovaries in the ewe caused the animal to become pseudopregnant and fluid to accumulate in the uterus. A preliminary electrophoretic examination of a specimen of this fluid at pH 3-5 showed prominent protein bands migrating as do the uterine proteins reported by Roberts & Parker (1974a). A similar result is obtained with intact animals treated with large amounts of exogenous progesterone, and the uterine fluid of such animals was therefore investigated more extensively.…”

Section: Introductionsupporting

confidence: 75%

“…The exploratory work of Roberts & Parker (1974a) and Laster (1977) has demonstrated that uterine proteins are present in the cow. Initially the uterine washings used by Roberts & Parker (1974a) provided a source of uterine protein but it rapidly became apparent that, with an average yield of 15 mg total protein per uterine washing, of which not more than 300 pg were the materials sought, this source was inadequate.…”

Section: Introductionmentioning

confidence: 99%

“…Initially the uterine washings used by Roberts & Parker (1974a) provided a source of uterine protein but it rapidly became apparent that, with an average yield of 15 mg total protein per uterine washing, of which not more than 300 pg were the materials sought, this source was inadequate. Roberts & Parker (1976) Harrison, Heap, Horton & Poyser (1972) showed that surgical separation of the uterus and the ovaries in the ewe caused the animal to become pseudopregnant and fluid to accumulate in the uterus.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proteins in the uterine secretions of the cow

Dixon¹,

Gibbons²

1979

Reproduction

View full text Add to dashboard Cite

Summary. Cows of normal reproductive history were treated with progesterone for periods of 2\p=n-\3months. The uterine secretions yielded 5 major fractions of macromolecular components. Two of the fractions comprised serum proteins. In the other 3 fractions at least 9 non-serum proteins were observed, 7 in one fraction, but in only small amounts. Of the remaining 2 non-serum proteins one is an acid phosphatase recoverable in small amounts, and the other is lactoferrin and is the major non-serum protein present in the uterine secretions of progesterone-treated cows.

show abstract

Section: Introductionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proteins in the uterine secretions of the cow

Dixon¹,

Gibbons²

1979

Reproduction

View full text Add to dashboard Cite

show abstract

“…Antiserum to bovine serum albumin was produced as described previously (Roberts & Parker, 1974). Antisera to tubulin and actin were generously provided by Dr R. A. Badley, who produced them as described previously (Badley et al, 1978).…”

Section: Methodsmentioning

confidence: 99%

Surface antigens on human melanoma cells studied with heterologous antisera

Roberts¹

1980

Br J Cancer

View full text Add to dashboard Cite

Summary.-Antisera were raised in rabbits to 6 human melanoma cell lines. The cell-surface antigens recognized by these antisera were examined using cell-surface labelling with 1251, followed by immunoprecipitation of soluble extracts of the cells and polyacrylamide-gel electrophoresis of the immunoprecipitates in the presence of sodium dodecyl sulphate (SDS). Up to 16 cell-surface antigens were recognized by these antisera, and 5 of the melanoma cell lines had a similar profile of cell-surface antigens. Digestion of labelled melanoma cells with neuraminidase before immunoprecipitation revealed that 8 of the larger antigens were sialoglycoproteins. The melanoma antisera produced haemagglutination of human erythrocytes at high dilutions, but the antigens involved could not be detected by iodination. In contrast, absorption of the melanoma sera with lymphocytes and fibroblasts revealed that these cells did contain some cell-surface glycoproteins in common with melanoma cells. The melanoma antisera contained antibodies to foetal calf serum proteins, but the amounts of these proteins on the surface of melanoma-cells were very low. Immunoprecipitation of labelled melanoma cell extracts with monospecific antiserum to 32-microglobulin produced 2 bands with mol. wts corresponding to 32-microglobulin and the HLA-determinant polypeptide chain. After absorption of melanoma antisera with cross-linked foetal calf serum, erythrocytes, lymphocytes and fibroblasts, antibodies against 10 labelled antigens remained in the absorbed antisera. However, antibodies against 8 of these antigens were still detected after absorption of the melanoma antisera with melanoma cells.

show abstract

“…This organism has high iron requirements for in vitro cultivation (50-100 μM) [Tachezy et al, 1996], surpassing those of eukaryotic cells, although comparable to other anaerobic amitochondriate protists. T. foetus inhabits the vagina, cervix, and the lumen of the bovine uterus, with the last one being characterized as rich in TF [Roberts and Parker, 1974]. Therefore, TF could be an important source of iron for T. foetus.…”

Section: Tritrichomonas Foetusmentioning

confidence: 99%